Changes of fractional anisotropy and RGMa in crossed cerebellar diaschisis induced by middle cerebral artery occlusion

  • Authors:
    • Yong Zhang
    • Xiao Wang
    • Jingliang Cheng
    • Yanan Lin
    • Lu Yang
    • Zhenghao Cao
    • Yunjun Yang
  • View Affiliations

  • Published online on: September 9, 2019     https://doi.org/10.3892/etm.2019.7986
  • Pages: 3595-3602
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Abstract

Crossed cerebellar diaschisis (CCD) is the phenomenon of hypoperfusion and hypometabolism of the contralateral cerebellar hemisphere caused by dysfunction of the associated supratentorial region. The aim of the present study was to analyze the changes in fractional anisotropy (FA) in CCD induced by middle cerebral artery occlusion (MCAO) using magnetic resonance‑diffusion tensor imaging (MR‑DTI). Furthermore, the role of repulsive guidance molecule a (RGMa) in CCD was assessed by measuring RGMa expression using histochemical analysis. In the present study, the cerebellar hemisphere was serially scanned with T2‑weighted, serial diffusion‑weighted and diffusion tensor (DT) imaging using a 3.0T GE Signa HDxt Scanner to analyze the changes in FA over 72 h. Subsequently, immunohistochemistry analyses of the corresponding cerebellar hemisphere sections were performed to assess the expression of RGMa. Results indicated that FA of both sides of the cerebellar hemisphere, particularly that of the contralateral cerebellar hemisphere (right side) derived from DTI, was reduced during the 72‑h time period following MCAO, and the decrease was maximal and statistically significant at 12 h (P<0.05). Immunohistochemistry analysis revealed a significant increase in the expression of RGMa protein in the affected region of the contralateral cerebellar hemisphere (right side) at 24 h following MCAO injury (P<0.05). Furthermore, the expression of RGMa and FA was negatively correlated in MCAO (P<0.05). The results suggest that MR‑DTI is an important assessment to evaluate changes of FA in CCD induced by MCAO. Furthermore, the present results suggest that RGMa, which was negatively correlated with FA in MCAO rats, may serve an important role in CCD.
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November-2019
Volume 18 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang Y, Wang X, Cheng J, Lin Y, Yang L, Cao Z and Yang Y: Changes of fractional anisotropy and RGMa in crossed cerebellar diaschisis induced by middle cerebral artery occlusion. Exp Ther Med 18: 3595-3602, 2019.
APA
Zhang, Y., Wang, X., Cheng, J., Lin, Y., Yang, L., Cao, Z., & Yang, Y. (2019). Changes of fractional anisotropy and RGMa in crossed cerebellar diaschisis induced by middle cerebral artery occlusion. Experimental and Therapeutic Medicine, 18, 3595-3602. https://doi.org/10.3892/etm.2019.7986
MLA
Zhang, Y., Wang, X., Cheng, J., Lin, Y., Yang, L., Cao, Z., Yang, Y."Changes of fractional anisotropy and RGMa in crossed cerebellar diaschisis induced by middle cerebral artery occlusion". Experimental and Therapeutic Medicine 18.5 (2019): 3595-3602.
Chicago
Zhang, Y., Wang, X., Cheng, J., Lin, Y., Yang, L., Cao, Z., Yang, Y."Changes of fractional anisotropy and RGMa in crossed cerebellar diaschisis induced by middle cerebral artery occlusion". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3595-3602. https://doi.org/10.3892/etm.2019.7986