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Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus

  • Authors:
    • Ruixian Zhang
    • Ya Li
    • Bangpin Pan
    • Yi Li
    • Aimin Liu
    • Xiaolan Li
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China, Basic Nursing Teaching Department of Kunming Medical University, Kunming, Yunnan 650500, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4067-4075
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    Published online on: September 17, 2019
       https://doi.org/10.3892/etm.2019.8013
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Abstract

Systemic lupus erythematosus (SLE) is a prototypical autoimmune disease, characterized by overactive inflammation and aberrant activation of lymphocytes. Chemokine C‑X‑C motif ligand 10 (CXCL10) has an important role in the initiation and deterioration of SLE. However, the expression levels of CXCL10 mRNA in T‑helper (Th) cells and B lymphocytes from SLE patients have remained elusive. In the present study, a Bioinformatics analysis of differentially expressed gene (DEG) profiles obtained from RNA sequencing data for three matched samples was performed to explore the hub genes, mainly through Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes and protein‑protein interaction analysis. Furthermore, the expression of CXCL10 in peripheral blood mononuclear cells (PBMCs), CD4+ Th cells and CD19+ B cells of 108 subjects, including 66 SLE patients and 42 healthy controls, was confirmed by reverse transcription‑quantitative PCR. In addition, 4 single‑nucleotide polymorphism (SNP) loci in the 3'‑untranslated region of CXCL10 were assessed using the Snapshot SNP genotyping assay. A total of 152 clustered DEGs mainly accumulated in immune‑associated GO terms and interferon‑associated pathways were identified. The expression of CXCL10, one of the central genes in the interaction network cluster (the degree of interaction, MCODE score=28.414), was 6.27‑fold higher in SLE patients compared with control patients. Furthermore, CXCL10 mRNA was confirmed to be elevated in PBMCs and CD19+ B cells of patients with SLE (P<0.001 for the two cell types). However, no significant difference in CD4+ T lymphocytes was present (P=0.881). In addition, no polymorphism was identified in four selected loci from the samples. Taken together, the present results demonstrated that CXCL10, one of the hub genes in the pathogenesis of SLE, is upregulated in PBMCs and B lymphocytes of patients with SLE, although none of the SNPs selected for analysis in the present study were identified to have any potential associations with SLE.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Li Y, Pan B, Li Y, Liu A and Li X: Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. Exp Ther Med 18: 4067-4075, 2019.
APA
Zhang, R., Li, Y., Pan, B., Li, Y., Liu, A., & Li, X. (2019). Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. Experimental and Therapeutic Medicine, 18, 4067-4075. https://doi.org/10.3892/etm.2019.8013
MLA
Zhang, R., Li, Y., Pan, B., Li, Y., Liu, A., Li, X."Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 18.5 (2019): 4067-4075.
Chicago
Zhang, R., Li, Y., Pan, B., Li, Y., Liu, A., Li, X."Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 18, no. 5 (2019): 4067-4075. https://doi.org/10.3892/etm.2019.8013
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Li Y, Pan B, Li Y, Liu A and Li X: Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. Exp Ther Med 18: 4067-4075, 2019.
APA
Zhang, R., Li, Y., Pan, B., Li, Y., Liu, A., & Li, X. (2019). Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. Experimental and Therapeutic Medicine, 18, 4067-4075. https://doi.org/10.3892/etm.2019.8013
MLA
Zhang, R., Li, Y., Pan, B., Li, Y., Liu, A., Li, X."Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 18.5 (2019): 4067-4075.
Chicago
Zhang, R., Li, Y., Pan, B., Li, Y., Liu, A., Li, X."Increased expression of hub gene CXCL10 in peripheral blood mononuclear cells of patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 18, no. 5 (2019): 4067-4075. https://doi.org/10.3892/etm.2019.8013
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