Open Access

Low‑frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic rats

  • Authors:
    • Lang Chen
    • Qian Zheng
    • Xianzhuo Chen
    • Jun Wang
    • Lan Wang
  • View Affiliations

  • Published online on: September 25, 2019     https://doi.org/10.3892/etm.2019.8051
  • Pages: 4040-4048
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetes is a chronic metabolic disease with a high prevalence worldwide, which typically delays or impairs wound healing, potentially causing death. Low‑frequency ultrasound treatment promotes the repair of various injuries and may promote wound healing. The aim of the present study was to determine whether low‑frequency ultrasound can accelerate wound healing, as well as investigate its effects on the expression of vascular endothelial growth factor (VEGF), transforming growth factor (TGF)‑β1, interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α in diabetic rats. A total of 45 Wistar rats were intraperitoneally injected with 1% streptozocin following intraperitoneal injection of pentobarbital sodium anesthesia. Subsequently an incision wound was created in the skin of back. The area of the wound was recorded to calculate the rate of wound healing. The expression of VEGF and TGF‑β1 was determined via immunohistochemical analysis and their mRNA and protein levels were measured via reverse transcription‑quantitative PCR analysis. The results revealed that when compared with the control group, low‑frequency ultrasound treatment significantly increased wound healing rate in diabetic rats and markedly increased the mRNA and protein levels of VEGF and TGF‑β1. US treatment also reduced the mRNA and protein levels of TNF‑α and IL‑6. In conclusion, the results of the present study indicated that low‑frequency ultrasound promotes the expression of VEGF and TGF‑β1, and inhibits the expression of IL‑6 and TNF‑α, thereby promoting wound healing in diabetic rats.
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November-2019
Volume 18 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Chen L, Zheng Q, Chen X, Wang J and Wang L: Low‑frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic rats. Exp Ther Med 18: 4040-4048, 2019.
APA
Chen, L., Zheng, Q., Chen, X., Wang, J., & Wang, L. (2019). Low‑frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic rats. Experimental and Therapeutic Medicine, 18, 4040-4048. https://doi.org/10.3892/etm.2019.8051
MLA
Chen, L., Zheng, Q., Chen, X., Wang, J., Wang, L."Low‑frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic rats". Experimental and Therapeutic Medicine 18.5 (2019): 4040-4048.
Chicago
Chen, L., Zheng, Q., Chen, X., Wang, J., Wang, L."Low‑frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic rats". Experimental and Therapeutic Medicine 18, no. 5 (2019): 4040-4048. https://doi.org/10.3892/etm.2019.8051