Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2019 Volume 18 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2019 Volume 18 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1

  • Authors:
    • Xu Cai
    • Na Yu
    • Jiangwei Ma
    • Wen‑Yang Li
    • Mingtao Xu
    • Erran Li
    • Min Zhang
    • Wei Wang
    • Yu Chen
    • Jian Kang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Respiratory Medicine, Heilongjiang Provincial Hospital, Harbin, Heilongjiang 150036, P.R. China, Department of Respiratory Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4368-4378
    |
    Published online on: October 14, 2019
       https://doi.org/10.3892/etm.2019.8102
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In immunosuppressed hosts, Legionella pneumophila (Lp) infection usually develops into severe pneumonia, which is pathologically characterized by increased vascular permeability and pulmonary edema. At present, mechanisms associated with changes in pulmonary capillary permeability (PCP) and the pathogenesis of pulmonary edema in immunosuppressed hosts with Lp infection are unclear. Therefore, in the present study an animal model of normal and immunosuppressed guinea pigs infected with Lp was established. An isolated perfused lung system was used to investigate the extent of changes in PCP. Pathological and immunofluorescence examinations were performed to explore the mechanism underlying these changes. The results indicated that PCP increased with the highest magnitude in immunosuppressed guinea pigs infected with Lp, with repeated ANOVA indicating synergism between infection and immunosuppression (P=0.0444). Hematoxylin and eosin staining and electron microscopy revealed more severe morphological damages in the lung tissues and pulmonary capillaries of the immunosuppressed animals infected with Lp compared with normal animals infected with Lp. Immunofluorescence analysis showed that immunosuppression reduced the expression of the vascular endothelial cell junction protein VE‑cadherin (P=0.027). Following Lp infection, VE‑cadherin expression was significantly lower in the immunosuppressed guinea pigs compared with their immunocompetent counterparts (P=0.001). These results suggest that immunosuppression combined with Lp infection induces more significant damage to pulmonary capillaries compared with Lp infection alone, resulting in a significantly increased PCP.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Stout JE and Yu VL: Legionellosis. New Eng J Med. 337:682–687. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Helms CM, Viner JP, Weisenburger DD, Chiu LC, Renner ED and Johnson W: Sporadic legionnaires' disease: Clinical observations on 87 nosocomial and community-acquired cases. Am J Med Sci. 288:2–12. 1984. View Article : Google Scholar : PubMed/NCBI

3 

Pedro-Botet ML, Sabria-Leal M, Sopena N, Manterola JM, Morera J, Blavia R, Padilla E, Matas L and Gimeno JM: Role of immunosuppression in the evolution of Legionnaires' disease. Clin Infect Dis. 26:14–19. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Beaute J, Zucs P and de Jong B; European Legionnaires' Disease Surveillance Network, : Legionnaires disease in Europe, 2009–2010. Euro Surveill. 18:204172013. View Article : Google Scholar : PubMed/NCBI

5 

Clark SB and Soos MP: Noncardiogenic Pulmonary Edema. StatPearls. StatPearls Publishing LLC. (Treasure Island (FL)). 2019.https://www.ncbi.nlm.nih.gov/books/NBK542230/

6 

Htwe TH and Khardori NM: Legionnaire's disease and immunosuppressive drugs. Infect Dis Clin North Am. 31:29–42. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Lo SK, Everitt J, Gu J and Malik AB: Tumor necrosis factor mediates experimental pulmonary edema by ICAM-1 and CD18-dependent mechanisms. J Clin Invest. 89:981–988. 1992. View Article : Google Scholar : PubMed/NCBI

8 

Donners MM, Wolfs IM, Olieslagers S, Mohammadi-Motahhari Z, Tchaikovski V, Heeneman S, van Buul JD, Caolo V, Molin DG, Post MJ and Waltenberger J: A disintegrin and metalloprotease 10 is a novel mediator of vascular endothelial growth factor-induced endothelial cell function in angiogenesis and is associated with atherosclerosis. Arterioscler Thromb Vasc Biol. 30:2188–2195. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Edelstein PH: Legionella jamestowniensis fatal pneumonia in an immunosuppressed man. J Infect Chemother. 23:59–61. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Nanovic Z: Legionnaires' disease and use of tumor necrosis factor-αlpha inhibitors: A forthcoming problem? Macedonian. J Med Sci. 6:465–472. 2013.

11 

Kirkpatrick WR, McAtee RK, Fothergill AW, Rinaldi MG and Patterson TF: Efficacy of voriconazole in a guinea pig model of disseminated invasive aspergillosis. Antimicrob Agents Chemother. 44:2865–2868. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Gamradt P, Xu Y, Gratz N, Duncan K, Kobzik L, Högler S, Kovarik P, Decker T and Jamieson AM: The influence of programmed cell death in myeloid cells on host resilience to infection with legionella pneumophila or streptococcus pyogenes. PLoS Pathog. 12:e10060322016. View Article : Google Scholar : PubMed/NCBI

13 

Ismael-Badarneh R, Guetta J, Klorin G, Berger G, Abu-Saleh N, Abassi Z and Azzam ZS: The role of angiotensin II and cyclic AMP in alveolar active sodium transport. PLoS One. 10:e01341752015. View Article : Google Scholar : PubMed/NCBI

14 

Ong HX, Benaouda F, Traini D, Cipolla D, Gonda I, Bebawy M, Forbes B and Young PM: In vitro and ex vivo methods predict the enhanced lung residence time of liposomal ciprofloxacin formulations for nebulisation. Eur J Phar Biopharm. 86:83–89. 2014. View Article : Google Scholar

15 

Kadlecek S, Shaghaghi H, Siddiqui S, Profka H, Pourfathi M and Rizi R: The effect of exogenous substrate concentrations on true and apparent metabolism of hyperpolarized pyruvate in the isolated perfused lung. NMR Biomed. 27:1557–1570. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Dominguez-Fandos D, Valdes C, Ferrer E, Puig-Pey R, Blanco I, Tura-Ceide O, Paul T, Peinado VI and Barberà JA: Sildenafil in a cigarette smoke-induced model of COPD in the guinea-pig. Eur Respir J. 46:346–354. 2015. View Article : Google Scholar : PubMed/NCBI

17 

He X, Liang Y, LaValley MP, Lai J and Ingalls RR: Comparative analysis of the growth and biological activity of a respiratory and atheroma isolate of chlamydia pneumoniae reveals strain-dependent differences in inflammatory activity and innate immune evasion. BMC Microbiol. 15:2282015. View Article : Google Scholar : PubMed/NCBI

18 

Wang E, Simard M, Ouellet N, Bergeron Y, Beauchamp D and Bergeron MG: Pathogenesis of pneumococcal pneumonia in cyclophosphamide-induced leukopenia in mice. Infect Immun. 70:4226–4238. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Machireddy K, Myint Z, Dein E, Mathai SC, Seo P, Haque U, Manno R and Timlin H: Mycophenolate mofetil in a lupus patient with pulmonary hypertension. Cureus. 10:e21212018.PubMed/NCBI

20 

Jais X, Launay D, Yaici A, Le Pavec J, Tchérakian C, Sitbon O, Simonneau G and Humbert M: Immunosuppressive therapy in lupus- and mixed connective tissue disease-associated pulmonary arterial hypertension: A retrospective analysis of twenty-three cases. Arthritis Rheum. 58:521–531. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Bates DO: Vascular endothelial growth factors and vascular permeability. Cardiovas Res. 87:262–271. 2010. View Article : Google Scholar

22 

Dejana E and Giampietro C: Vascular endothelial-cadherin and vascular stability. Curr Opin Hematol. 19:218–223. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Komarova Y and Malik AB: Regulation of endothelial permeability via paracellular and transcellular transport pathways. Ann Rev Physiol. 72:463–493. 2010. View Article : Google Scholar

24 

Komarova YA, Mehta D and Malik AB: Dual regulation of endothelial junctional permeability. Sci STKE. 2007:re82007. View Article : Google Scholar : PubMed/NCBI

25 

Weiss SJ: Tissue destruction by neutrophils. N Engl J Med. 320:365–376. 1989. View Article : Google Scholar : PubMed/NCBI

26 

Lampugnani MG, Resnati M, Raiteri M, Pigott R, Pisacane A, Houen G, Ruco LP and Dejana E: A novel endothelial-specific membrane protein is a marker of cell-cell contacts. J Cell Biol. 118:1511–1522. 1992. View Article : Google Scholar : PubMed/NCBI

27 

Lan CC, Peng CK, Tang SE, Wu SY, Huang KL and Wu CP: Anti-vascular endothelial growth factor antibody suppresses ERK and NF-κB activation in ischemia-reperfusion lung injury. PLoS One. 11:e01599222016. View Article : Google Scholar : PubMed/NCBI

28 

Rieg AD, Suleiman S, Perez-Bouza A, Braunschweig T, Spillner JW, Schröder T, Verjans E, Schälte G, Rossaint R, Uhlig S and Martin C: Milrinone relaxes pulmonary veins in guinea pigs and humans. PLoS One. 9:e876852014. View Article : Google Scholar : PubMed/NCBI

29 

Viswanathan VK, Edelstein PH, Pope CD and Cianciotto NP: The legionella pneumophila iraAB locus is required for iron assimilation, intracellular infection, and virulence. Infect Immun. 68:1069–1079. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Edelstein PH: The guinea pig model of legionnaires' disease. Methods Mol Biol. 954:521–540. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Wang X, Ma S, Liu Y, Xu W and Li Z: Effects and mechanism analysis of combined infusion by levosimendan and vasopressin on acute lung injury in rats septic shock. Cell Biochem Biophys. 70:1639–1645. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Daudel F, Tuller D, Krahenbuhl S, Jakob SM and Takala J: Pulse pressure variation and volume responsiveness during acutely increased pulmonary artery pressure: An experimental study. Crit Care. 14:R1222010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Cai X, Yu N, Ma J, Li WY, Xu M, Li E, Zhang M, Wang W, Chen Y, Kang J, Kang J, et al: Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1. Exp Ther Med 18: 4368-4378, 2019.
APA
Cai, X., Yu, N., Ma, J., Li, W., Xu, M., Li, E. ... Kang, J. (2019). Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1. Experimental and Therapeutic Medicine, 18, 4368-4378. https://doi.org/10.3892/etm.2019.8102
MLA
Cai, X., Yu, N., Ma, J., Li, W., Xu, M., Li, E., Zhang, M., Wang, W., Chen, Y., Kang, J."Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1". Experimental and Therapeutic Medicine 18.6 (2019): 4368-4378.
Chicago
Cai, X., Yu, N., Ma, J., Li, W., Xu, M., Li, E., Zhang, M., Wang, W., Chen, Y., Kang, J."Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4368-4378. https://doi.org/10.3892/etm.2019.8102
Copy and paste a formatted citation
x
Spandidos Publications style
Cai X, Yu N, Ma J, Li WY, Xu M, Li E, Zhang M, Wang W, Chen Y, Kang J, Kang J, et al: Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1. Exp Ther Med 18: 4368-4378, 2019.
APA
Cai, X., Yu, N., Ma, J., Li, W., Xu, M., Li, E. ... Kang, J. (2019). Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1. Experimental and Therapeutic Medicine, 18, 4368-4378. https://doi.org/10.3892/etm.2019.8102
MLA
Cai, X., Yu, N., Ma, J., Li, W., Xu, M., Li, E., Zhang, M., Wang, W., Chen, Y., Kang, J."Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1". Experimental and Therapeutic Medicine 18.6 (2019): 4368-4378.
Chicago
Cai, X., Yu, N., Ma, J., Li, W., Xu, M., Li, E., Zhang, M., Wang, W., Chen, Y., Kang, J."Altered pulmonary capillary permeability in immunosuppressed guinea pigs infected with Legionella pneumophila serogroup 1". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4368-4378. https://doi.org/10.3892/etm.2019.8102
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team