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Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37

  • Authors:
    • Teodor Ștefanache
    • Norina Forna
    • Magda Bădescu
    • Daniela Jitaru
    • Mihaiela Loredana Dragos
    • Ciprian Rezuș
    • Bogdan Mihail Diaconescu
    • Oana Bădulescu
    • Elena Rezuș
    • Manuela Ciocoiu
    • Codruta Bădescu
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, University of Medicine and Pharmacy ‘Grigore T. Popa’, 700115 Iaşi, Romania, Department of Implantology, Dental Medicine, University of Medicine and Pharmacy ‘Grigore T. Popa’, 700115 Iaşi, Romania, Regional Institute of Oncology Iasi, 700115 Iaşi, Romania, Department of Internal Medicine, University of Medicine and Pharmacy ‘Grigore T. Popa’, 700115 Iaşi, Romania, Rehabilitation Hospital of Iasi, Rheumatology Clinic, University of Medicine and Pharmacy ‘Grigore T. Popa’, 700115 Iaşi, Romania
    Copyright: © Ștefanache et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5033-5040
    |
    Published online on: October 21, 2019
       https://doi.org/10.3892/etm.2019.8117
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Abstract

It is common knowledge that some natural antimicrobial peptides also have a tumoricidal effect. We have shown that the peptides defensin and cathelicidin LL37 have cytostatic effects on human tumor cell lines HT29 (colorectal carcinoma) and A549 (alveolar carcinoma). In order to determine the modulating mechanism of these peptides we assessed the gene expression of the AKT, HIF‑1α, XBP, NRF2, PERK, CHOP, BCL2, IRE1α and PI3K molecular targets involved in the survival, growth, proliferation and apoptosis pathways of tumor cells in the presence or absence of the studied peptides. Thus, this research enabled us to determine molecular markers and methods of assessment and monitoring of tumor cell cytotoxicity by high‑performance molecular biology techniques. Defensin and cathelicidin LL37 activated tumor cell apoptosis, especially for the HT29, but also for A549 line, by increasing gene expression of CHOP and by lowering BCL2 gene expression. Oxidative stress determined the increase in gene expression of XBP, which directly influenced CHOP. The decrease in NRF2 gene expression highlighted the inhibition of cell proliferation, while the decrease in HIF1α gene expression evidenced the decrease in cell survival.
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1 

Hilchie AL, Vale R, Zemlak TS and Hoskin DW: Generation of a hematologic malignancy-selective membranolytic peptide from the antimicrobial core (RRWQWR) of bovine lactoferricin. Exp Mol Pathol. 95:192–198. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Sato H and Feix JB: Peptide-membrane interactions and mechanisms of membrane destruction by amphipathic α-helical antimicrobial peptides. Biochim Biophys Acta. 1758:1245–1256. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Koczulla AR and Bals R: Antimicrobial peptides: Current status and therapeutic potential. Drugs. 63:389–406. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Homayouni-Tabrizi M, Asoodeh A, Soltani M and Forghanifard MM: Antimicrobial peptide Brevinin-2R induces the secretion of a pro-inflamatory cytokine in HepG2 cells. J Bas Res Med Sci. 2:23–29. 2015.

5 

Karadag R, Bayram N, Oguztuzun S, Bayramlar H, Bozer B, Simsek G and Rapuano CJ: An investigation of human beta-defensins and cathelicidin expression in patients with pterygium. Arq Bras Oftalmol. 80:277–280. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Zhuravel E, Shestakova T, Efanova O, Yusefovich Y, Lytvin D, Soldatkina M and Pogrebnoy P: Human beta-defensin-2 controls cell cycle in malignant epithelial cells: In vitro study. Exp Oncol. 33:114–120. 2011.PubMed/NCBI

7 

He M, Zhang H, Li Y, Wang G, Tang B, Zhao J, Huang Y and Zheng J: Cathelicidin-derived antimicrobial peptides inhibit zika virus through direct inactivation and interferon pathway. Front Immunol. 9:7222018. View Article : Google Scholar : PubMed/NCBI

8 

Vermes I, Haanen C and Reutelingsperger C: Flow cytometry of apoptotic cell death. J Immunol Methods. 243:167–190. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Schleicher SM, Moretti L, Varki V and Lu B: Progress in the unraveling of the endoplasmic reticulum stress/autophagy pathway and cancer: Implications for future therapeutic approaches. Drug Resist Updat. 13:79–86. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Voiculescu VM, Caruntu C, Solomon I, Lupu M, Ilie MA, Boda D, Constantin C and Neagu M: Squamous cell carcinoma: Biomarkers and potential therapeutic targetsHuman Skin Cancers-Pathways, Mechanisms, Targets and Treatments. Blumenberg M: IntechOpen; London: pp. 135–159. 2018

11 

Neagu M, Caruntu C, Constantin C, Boda D, Zurac S, Spandidos DA and Tsatsakis AM: Chemically induced skin carcinogenesis: Updates in experimental models (Review). Oncol Rep. 35:2516–2528. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Boda D: Cellomics as integrative omics for cancer. Curr Proteomics. 10:237–245. 2013. View Article : Google Scholar

13 

Neagu M, Constantin C, Tanase C and Boda D: Patented biomarker panels in early detection of cancer. Recent Pat Biomark. 1:10–24. 2011. View Article : Google Scholar

14 

Kraskiewicz H and FitzGerald U: InterfERing with endoplasmic reticulum stress. Trends Pharmacol Sci. 33:53–63. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Zinszner H, Kuroda M, Wang X, Batchvarova N, Lightfoot RT, Remotti H, Stevens JL and Ron D: CHOP is implicated in programmed cell death in response to impaired function of the endoplasmic reticulum. Genes Dev. 12:982–995. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Park SH, Choi HJ, Yang H, Do KH, Kim J, Lee DW and Moon Y: Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma. J Biol Chem. 285:35330–35339. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Lupu M, Caruntu A, Caruntu C, Papagheorghe LML, Ilie MA, Voiculescu V, Boda D, Constantin C, Tanase C, Sifaki M, Drakoulis N, Mamoulakis C, Tzanakakis G, Neagu M, Spandidos DA, Izotov B and Tsatsakis A: Neuroendocrine factors: The missing link in non melanoma skin cancer. Oncol Rep. 38:1327–13401. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Boda D, Docea AO, Calina D, Ilie MA, Caruntu C, Zurac S, Neagu M, Constantin C, Branisteanu DE, Voiculescu V, et al: Human papilloma virus: Apprehending the link with carcinogenesis and unveiling new research avenues (Review). Int J Oncol. 52:637–655. 2018.PubMed/NCBI

19 

Solomon I, Voiculescu VM, Caruntu C, Lupu M, Popa A, Ilie MA, Albulescu R, Caruntu A, Tanase C, Constantin C, et al: Neuroendocrine factors and head and neck squamous cell carcinoma: An affair to remember. Dis Markers. 2018:97878312018. View Article : Google Scholar : PubMed/NCBI

20 

Healy SJ, Gorman AM, Mousavi-Shafaei P, Gupta S and Samali A: Targeting the endoplasmic reticulum-stress response as an anticancer strategy. Eur J Pharmacol. 625:234–246. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Cioplea M, Caruntu C, Zurac S, Bastian A, Sticlaru L, Cioroianu A, Boda D, Jugulete G, Nichita L and Popp C: Dendritic cell distribution in mycosis fungoides vs. inflammatory dermatosis and other T-cell skin lymphoma. Oncol Lett. 17:4055–4059. 2019.PubMed/NCBI

22 

Del Poeta G, Venditti A, Del Principe MI, Maurillo L, Buccisano F, Tamburini A, Cox MC, Franchi A, Bruno A, Mazzone C, et al: Amount of spontaneous apoptosis detected by Bax/Bcl-2 ratio predicts outcome in acute myeloid leukemia (AML). Blood. 101:2125–2131. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Mitsuishi Y, Taguchi K, Kawatani Y, Shibata T, Nukiwa T, Aburatani H, Yamamoto M and Motohashi H: Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Cancer Cell. 22:66–79. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Ren D, Villeneuve NF, Jiang T, Wu T, Lau A, Toppin HA and Zhang DD: Brusatol enhances the efficacy of chemotherapy by inhibiting the Nrf2-mediated defense mechanism. Proc Natl Acad Sci USA. 108:1433–1438. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Magesh S, Chen Y and Hu L: Small molecule modulators of Keap1-Nrf2-ARE pathway as potential preventive and therapeutic agents. Med Res Rev. 32:687–726. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Kong D, Park EJ, Stephen AG, Calvani M, Cardellina JH, Monks A, Fisher RJ, Shoemaker RH and Melillo G: Echinomycin, a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity. Cancer Res. 65:9047–9055. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ștefanache T, Forna N, Bădescu M, Jitaru D, Dragos ML, Rezuș C, Diaconescu BM, Bădulescu O, Rezuș E, Ciocoiu M, Ciocoiu M, et al: Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37. Exp Ther Med 18: 5033-5040, 2019.
APA
Ștefanache, T., Forna, N., Bădescu, M., Jitaru, D., Dragos, M.L., Rezuș, C. ... Bădescu, C. (2019). Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37. Experimental and Therapeutic Medicine, 18, 5033-5040. https://doi.org/10.3892/etm.2019.8117
MLA
Ștefanache, T., Forna, N., Bădescu, M., Jitaru, D., Dragos, M. L., Rezuș, C., Diaconescu, B. M., Bădulescu, O., Rezuș, E., Ciocoiu, M., Bădescu, C."Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37". Experimental and Therapeutic Medicine 18.6 (2019): 5033-5040.
Chicago
Ștefanache, T., Forna, N., Bădescu, M., Jitaru, D., Dragos, M. L., Rezuș, C., Diaconescu, B. M., Bădulescu, O., Rezuș, E., Ciocoiu, M., Bădescu, C."Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37". Experimental and Therapeutic Medicine 18, no. 6 (2019): 5033-5040. https://doi.org/10.3892/etm.2019.8117
Copy and paste a formatted citation
x
Spandidos Publications style
Ștefanache T, Forna N, Bădescu M, Jitaru D, Dragos ML, Rezuș C, Diaconescu BM, Bădulescu O, Rezuș E, Ciocoiu M, Ciocoiu M, et al: Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37. Exp Ther Med 18: 5033-5040, 2019.
APA
Ștefanache, T., Forna, N., Bădescu, M., Jitaru, D., Dragos, M.L., Rezuș, C. ... Bădescu, C. (2019). Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37. Experimental and Therapeutic Medicine, 18, 5033-5040. https://doi.org/10.3892/etm.2019.8117
MLA
Ștefanache, T., Forna, N., Bădescu, M., Jitaru, D., Dragos, M. L., Rezuș, C., Diaconescu, B. M., Bădulescu, O., Rezuș, E., Ciocoiu, M., Bădescu, C."Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37". Experimental and Therapeutic Medicine 18.6 (2019): 5033-5040.
Chicago
Ștefanache, T., Forna, N., Bădescu, M., Jitaru, D., Dragos, M. L., Rezuș, C., Diaconescu, B. M., Bădulescu, O., Rezuș, E., Ciocoiu, M., Bădescu, C."Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37". Experimental and Therapeutic Medicine 18, no. 6 (2019): 5033-5040. https://doi.org/10.3892/etm.2019.8117
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