Poly(lactic‑co‑glycolic acid)‑bioactive glass composites as nanoporous scaffolds for bone tissue engineering: In vitro and in vivo studies

Yang,Liuqing; Liu,Shuying; Fang,Wei; Chen,Jun; Chen,Yu

doi:10.3892/etm.2019.8121

Poly(lactic‑co‑glycolic acid)‑bioactive glass composites as nanoporous scaffolds for bone tissue engineering: In vitro and in vivo studies

Authors:
- Liuqing Yang
- Shuying Liu
- Wei Fang
- Jun Chen
- Yu Chen
View Affiliations

Affiliations: Department of Stomatology, Guangzhou Women and Children’s Medical Center, Guangzhou, Guangdong 510623, P.R. China, Department of Periodontics, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China
Published online on: October 23, 2019 https://doi.org/10.3892/etm.2019.8121
Pages: 4874-4880
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to investigate the feasibility of using composite scaffolds of poly(lactic‑co‑glycolic acid) (PLGA) and bioactive glass (BG) to repair bone defects. PLGA/BG composite scaffolds were prepared by thermally‑induced phase separation. Scanning electron microscopy (SEM) was used to study the morphology, and liquid (absolute ethanol) replacement was used to calculate the porosity of the scaffold. The biocompatibility and degradation of the scaffold were determined using human osteosarcoma cell line MG‑63 and animal experiments. SEM showed that the scaffold had a nanofibrous three‑dimensional network structure with a fiber diameter of 160‑320 nm, a pore size of 1‑7 µm, and a porosity of 93.048±0.121%. The scaffold structure was conducive to cell adhesion and proliferation. It promoted cell osteogenesis and could be stably degraded in vivo.

View Figures

View References

1	Furia JP, Rompe JD, Cacchio A and Maffulli N: Shock wave therapy as a treatment of nonunions, avascular necrosis, and delayed healing of stress fractures. Foot Ankle Clin. 15:651–662. 2010. View Article : Google Scholar : PubMed/NCBI
2	Lichte P, Pape HC, Pufe T, Kobbe P and Fischer H: Scaffolds for bone healing: Concepts, materials and evidence. Injury. 42:569–573. 2011. View Article : Google Scholar : PubMed/NCBI
3	Palecek SP, Loftus JC, Ginsberg MH, Lauffenburger DA and Horwitz AF: Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness. Nature. 385:537–540. 1997. View Article : Google Scholar : PubMed/NCBI
4	Félix Lanao RP, Leeuwenburgh SC, Wolke JG and Jansen JA: Bone response to fast-degrading, injectable calcium phosphate cements containing PLGA microparticles. Biomaterials. 32:8839–8847. 2011. View Article : Google Scholar : PubMed/NCBI
5	Chereddy KK, Vandermeulen G and Préat V: PLGA based drug delivery systems: Promising carriers for wound healing activity. Wound Repair Regen. 24:223–236. 2016. View Article : Google Scholar : PubMed/NCBI
6	Gao S, Tang G, Hua D, Xiong R, Han J, Jiang S, Zhang Q and Huang C: Stimuli-responsive bio-based polymeric systems and their applications. J Mater Chem B Mater Biol Med. 7:709–729. 2019. View Article : Google Scholar
7	Kido HW, Brassolatti P, Tim CR, Gabbai-Armelin PR, Magri AM, Fernandes KR, Bossini PS, Parizotto NA, Crovace MC, Malavazi I, et al: Porous poly (D,L-lactide-co-glycolide) acid/biosilicate^® composite scaffolds for bone tissue engineering. J Biomed Mater Res B Appl Biomater. 105:63–71. 2017. View Article : Google Scholar : PubMed/NCBI
8	Peitl O, Zanotto ED, Serbena FC and Hench LL: Compositional and microstructural design of highly bioactive P₂O₅-Na₂O-CaO-SiO₂ glass-ceramics. Acta Biomater. 8:321–332. 2012. View Article : Google Scholar : PubMed/NCBI
9	Granito RN, Rennó AC, Ravagnani C, Bossini PS, Mochiuti D, Jorgetti V, Driusso P, Peitl O, Zanotto ED, Parizotto NA, et al: In vivo biological performance of a novel highly bioactive glass-ceramic (Biosilicate^®): A biomechanical and histomorphometric study in rat tibial defects. J Biomed Mater Res B Appl Biomater. 97:139–147. 2011. View Article : Google Scholar : PubMed/NCBI
10	Habraken WJ, Wolke JG, Mikos AG and Jansen JA: PLGA microsphere/calcium phosphate cement composites for tissue engineering: In vitro release and degradation characteristics. J Biomater Sci Polym Ed. 19:1171–1188. 2008. View Article : Google Scholar : PubMed/NCBI
11	Ruhé PQ, Boerman OC, Russel FG, Mikos AG, Spauwen PH and Jansen JA: In vivo release of rhBMP-2 loaded porous calcium phosphate cement pretreated with albumin. J Mater Sci Mater Med. 17:919–927. 2006. View Article : Google Scholar : PubMed/NCBI
12	Ding J, Zhang J, Li J, Li D, Xiao C, Xiao H, Yang H, Zhuang X and Chen X: Electrospun polymer biomaterials. J.Prog Polym Sci. 90:1–34. 2019. View Article : Google Scholar
13	Plachokova A, Link D, van den Dolder J, van den Beucken J and Jansen J: Bone regenerative properties of injectable PGLA-CaP composite with TGF-beta1 in a rat augmentation model. J Tissue Eng Regen Med. 1:457–464. 2007. View Article : Google Scholar : PubMed/NCBI
14	Gu BK, Choi DJ, Park SJ, Kim YJ and Kim CH: 3D bioprinting technologies for tissue engineering applications. Adv Exp Med Biol. 1078:15–28. 2018. View Article : Google Scholar : PubMed/NCBI
15	Mastrogiacomo M, Scaglione S, Martinetti R, Dolcini L, Beltrame F, Cancedda R and Quarto R: Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics. Biomaterials. 27:3230–3237. 2006. View Article : Google Scholar : PubMed/NCBI
16	Deing A, Luthringer B, Laipple D, Ebel T and Willumeit R: A porous TiAl6V4 implant material for medical application. Int J Biomater. 2014:9042302014. View Article : Google Scholar : PubMed/NCBI
17	Chen G, Liu B, Liu H, Zhang H, Yang K, Wang Q, Ding J and Chang F: Calcium phosphate cement loaded with 10% vancomycin delivering high early and late local antibiotic concentration in vitro. Orthop Traumatol Surg Res. 104:1271–1275. 2018. View Article : Google Scholar : PubMed/NCBI
18	Jun HW, Paramonov SE, Dong H, Forraz N, McGuckin C and Hartgerink JD: Tuning the mechanical and bioresponsive properties of peptide-amphiphile nanofiber networks. J Biomater Sci Polym Ed. 19:665–676. 2008. View Article : Google Scholar : PubMed/NCBI
19	Murphy WL and Mooney DJ: Molecular-scale biomimicry. Nat Biotechnol. 20:30–31. 2002. View Article : Google Scholar : PubMed/NCBI
20	Proff P and Römer P: The molecular mechanism behind bone remodelling: A review. Clin Oral Investig. 13:355–362. 2009. View Article : Google Scholar : PubMed/NCBI
21	Zhu B, Xu W, Liu J, Ding J and Chen X: Osteoinductive agents-incorporated three-dimensional biphasic polymer scaffold for synergistic bone pegeneration. J. ACS Biomater Sci Eng. 5:986–995. 2019. View Article : Google Scholar
22	Fávaro-Pípi E, Bossini P, de Oliveira P, Ribeiro JU, Tim C, Parizotto NA, Alves JM, Ribeiro DA, Selistre de Araújo HS and Renno AC: Low-intensity pulsed ultrasound produced an increase of osteogenic genes expression during the process of bone healing in rats. Ultrasound Med Biol. 36:2057–2064. 2010. View Article : Google Scholar : PubMed/NCBI
23	Renno AC, van de Watering FC, Nejadnik MR, Crovace MC, Zanotto ED, Wolke JG, Jansen JA and van den Beucken JJ: Incorporation of bioactive glass in calcium phosphate cement: An evaluation. Acta Biomater. 9:5728–5739. 2013. View Article : Google Scholar : PubMed/NCBI
24	Xynos ID, Edgar AJ, Buttery LD, Hench LL and Polak JM: Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution. J Biomed Mater Res. 55:151–157. 2001. View Article : Google Scholar : PubMed/NCBI
25	Bellows CG, Aubin JE, Heersche JN and Antosz ME: Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif Tissue Int. 38:143–154. 1986. View Article : Google Scholar : PubMed/NCBI
26	Liu K, Cai J, Li H, Feng J, Feng C and Lu F: The disturbed function of neutrophils at the early stage of fat grafting impairs long-term fat graft retention. Plast Reconstr Surg. 142:1229–1238. 2018. View Article : Google Scholar : PubMed/NCBI
27	Mazio C, Casale C, Imparato G, Urciuolo F, Attanasio C, De Gregorio M, Rescigno F and Netti PA: Pre-vascularized dermis model for fast and functional anastomosis with host vasculature. Biomaterials. 192:159–170. 2019. View Article : Google Scholar : PubMed/NCBI
28	Barabaschi GD, Manoharan V, Li Q and Bertassoni LE: Engineering pre-vascularized scaffolds for bone regeneration. Adv Exp Med Biol. 881:79–94. 2015. View Article : Google Scholar : PubMed/NCBI
29	Turkevych M, Turkevych A, Kadjaya A, Gold MH, Lotti T and Sulamanidze G: Pathomorphological criteria of use efficiency of resorbable and permanent implants in aesthetic medicine and cosmetic dermatology. J Cosmet Dermatol. 17:731–735. 2018. View Article : Google Scholar : PubMed/NCBI