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MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9

  • Authors:
    • Hongqi Zhang
    • Yunjia Wang
    • Guanteng Yang
    • Honggui Yu
    • Zhenhai Zhou
    • Mingxing Tang
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4689-4697
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    Published online on: October 30, 2019
       https://doi.org/10.3892/etm.2019.8148
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Abstract

Cartilage injury is difficult to repair since the cartilage tissue lacks self‑restoration ability. Improved formation of chondrocytes differentiated from the mesenchymal stem cells (MSC) by genetic regulation is a potentially promising therapeutic option. SOX9 is a critical transcription factor for mesenchymal condensation prior to chondrogenesis. Previous studies demonstrated that several microRNAs (miRNAs or miRs) play a critical role in the chondrogenic differentiation of MSCs. However, the interactional relations between miR‑30a and SOX9 during chondrogenic differentiation of MSCs need to be further elucidated. In the present study, human bone marrow‑derived mesenchymal stem cells have been isolated and induced into chondrogenic differentiation to imitate the cartilage formation in vitro. Additionally, the expression levels of several miRNAs that were reported to interact with the SOX9 3'untranslated region (UTR) were examined by using reverse transcription‑quantitative PCR. The interactional relations between candidate miRNAs and SOX9 were verified with the transfection of a miRNA mimic or inhibitor and a luciferase reporter gene assay. The results indicate that miR‑30a and miR‑195 were consistently increased during MSC chondrogenic differentiation. Additionally, the binding of miR‑30a to the SOX9 3'UTR was verified. Then, the authors upregulated the expression of miR‑30a and found that MSC chondrogenic differentiation was inhibited. Taken together, the results of the present study demonstrate that miR‑30a has a negative regulatory effect on MSC chondrogenic differentiation by targeting SOX9. Advances in epigenetic regulating methods will likely be the future of systemic treatment of cartilage injury.
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View References

1 

Loeser RF, Collins JA and Diekman BO: Ageing and the pathogenesis of osteoarthritis. Nat Rev Rheumatol. 12:412–420. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Makris EA, Gomoll AH, Malizos KN, Hu JC and Athanasiou KA: Repair and tissue engineering techniques for articular cartilage. Nat Rev Rheumatol. 11:21–34. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Roos EM and Arden NK: Strategies for the prevention of knee osteoarthritis. Nat Rev Rheumatol. 12:92–101. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Bhattacharjee M, Coburn J, Centola M, Murab S, Barbero A, Kaplan DL, Martin I and Ghosh S: Tissue engineering strategies to study cartilage development, degeneration and regeneration. Adv Drug Deliv Rev. 84:107–122. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Evans CH and Huard J: Gene therapy approaches to regenerating the musculoskeletal system. Nat Rev Rheumatol. 11:234–242. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Craft AM, Rockel JS, Nartiss Y, Kandel RA, Alman BA and Keller GM: Generation of articular chondrocytes from human pluripotent stem cells. Nat Biotechnol. 33:638–645. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Xia H, Liang C, Luo P, Huang J, He J, Wang Z, Cao X, Peng C and Wu S: Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection. Stem Cell Res Ther. 9:1742018. View Article : Google Scholar : PubMed/NCBI

8 

Grayson WL, Bunnell BA, Martin E, Frazier T, Hung BP and Gimble JM: Stromal cells and stem cells in clinical bone regeneration. Nat Rev Endocrinol. 11:140–150. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Visweswaran M, Pohl S, Arfuso F, Newsholme P, Dilley R, Pervaiz S and Dharmarajan A: Multi-lineage differentiation of mesenchymal stem cells-To Wnt, or not Wnt. Int J Biochem Cell Biol. 68:139–147. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Jiang X, Huang X, Jiang T, Zheng L, Zhao J and Zhang X: The role of Sox9 in collagen hydrogel-mediated chondrogenic differentiation of adult mesenchymal stem cells (MSCs). Biomater Sci. 6:1556–1568. 2018. View Article : Google Scholar : PubMed/NCBI

11 

Loebel C, Czekanska EM, Bruderer M, Salzmann G, Alini M and Stoddart MJ: In vitro osteogenic potential of human mesenchymal stem cells is predicted by Runx2/Sox9 ratio. Tissue Eng Part A. 21:115–123. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Ono N, Ono W, Nagasawa T and Kronenberg HM: A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones. Nat Cell Biol. 16:1157–1167. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Shivdasani RA: MicroRNAs: Regulators of gene expression and cell differentiation. Blood. 108:3646–3653. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Treiber T, Treiber N and Meister G: Regulation of microRNA biogenesis and its crosstalk with other cellular pathways. Nat Rev Mol Cell Biol. 20:5–20. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Wa Q, He P, Huang S, Zuo J, Li X, Zhu J, Hong S, Lv G, Cai D, Xu D, et al: miR-30b regulates chondrogenic differentiation of mouse embryo-derived stem cells by targeting SOX9. Exp Ther Med. 14:6131–6137. 2017.PubMed/NCBI

16 

Anderson BA and McAlinden A: miR-483 targets SMAD4 to suppress chondrogenic differentiation of human mesenchymal stem cells. J Orthop Res. 35:2369–2377. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Lee S, Yoon DS, Paik S, Lee KM, Jang Y and Lee JW: microRNA-495 inhibits chondrogenic differentiation in human mesenchymal stem cells by targeting Sox9. Stem Cells Dev. 23:1798–1808. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Moutinho C and Esteller M: MicroRNAs and epigenetics. Adv Cancer Res. 135:189–220. 2017. View Article : Google Scholar : PubMed/NCBI

19 

van Meurs JB, Boer CG, Lopez-Delgado L and Riancho JA: Role of epigenomics in bone and cartilage disease. J Bone Miner Res. 34:215–230. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Chang T, Xie J, Li H, Li D, Liu P and Hu Y: MicroRNA-30a promotes extracellular matrix degradation in articular cartilage via downregulation of Sox9. Cell Prolif. 49:207–218. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Almeida MI, Silva AM, Vasconcelos DM, Almeida CR, Caires H, Pinto MT, Calin GA, Santos SG and Barbosa MA: miR-195 in human primary mesenchymal stromal/stem cells regulates proliferation, osteogenesis and paracrine effect on angiogenesis. Oncotarget. 7:7–22. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Liu S, Dong H, Dai H, Liu D and Wang Z: MicroRNA-216b regulated proliferation and invasion of non-small cell lung cancer by targeting SOX9. Oncol Lett. 15:10077–10083. 2018.PubMed/NCBI

23 

Liu XJ, Bai XG, Teng YL, Song L, Lu N and Yang RQ: miRNA-15a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis. Eur Rev Med Pharmacol Sci. 20:3319–3326. 2016.PubMed/NCBI

24 

Wang YJ, Zhang HQ, Han HL, Zou YY, Gao QL and Yang GT: Taxifolin enhances osteogenic differentiation of human bone marrow mesenchymal stem cells partially via NF-κB pathway. Biochem Biophys Res Commun. 490:36–43. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Uder C, Bruckner S, Winkler S, Tautenhahn HM and Christ B: Mammalian MSC from selected species: Features and applications. Cytometry A. 93:32–49. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Zhao C, Jiang W, Zhou N, Liao J, Yang M, Hu N, Liang X, Xu W, Chen H, Liu W, et al: Sox9 augments BMP2-induced chondrogenic differentiation by downregulating Smad7 in mesenchymal stem cells (MSCs). Genes Dis. 4:229–239. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Liao J, Hu N, Zhou N, Lin L, Zhao C, Yi S, Fan T, Bao W, Liang X, Chen H, et al: Sox9 potentiates BMP2-induced chondrogenic differentiation and inhibits BMP2-induced osteogenic differentiation. PLoS One. 9:e890252014. View Article : Google Scholar : PubMed/NCBI

29 

Almalki SG and Agrawal DK: Key transcription factors in the differentiation of mesenchymal stem cells. Differentiation. 92:41–51. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Chang M, Lin H, Fu H, Wang B, Han G and Fan M: MicroRNA-195-5p regulates osteogenic differentiation of periodontal ligament cells under mechanical loading. J Cell Physiol. 232:3762–3774. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Eguchi T, Watanabe K, Hara ES, Ono M, Kuboki T and Calderwood SK: OstemiR: A novel panel of microRNA biomarkers in osteoblastic and osteocytic differentiation from mesencymal stem cells. PLoS One. 8:e587962013. View Article : Google Scholar : PubMed/NCBI

32 

Luo Y, Sinkeviciute D, He Y, Karsdal M, Henrotin Y, Mobasheri A, Önnerfjord P and Bay-Jensen A: The minor collagens in articular cartilage. Protein Cell. 8:560–572. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Duan P and Bonewald LF: The role of the wnt/β-catenin signaling pathway in formation and maintenance of bone and teeth. Int J Biochem Cell Biol. 77:23–29. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Deng A, Zhang H, Hu M, Liu S, Wang Y, Gao Q and Guo C: The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation. Exp Ther Med. 15:789–794. 2018.PubMed/NCBI

35 

Deng ZH, Li YS, Gao X, Lei GH and Huard J: Bone morphogenetic proteins for articular cartilage regeneration. Osteoarthritis Cartilage. 26:1153–1161. 2018. View Article : Google Scholar : PubMed/NCBI

36 

Viswanathan C, Kulkarni R, Bopardikar A and Ramdasi S: Significance of CD34 negative hematopoietic stem cells and CD34 positive mesenchymal stem cells-A valuable dimension to the current understanding. Curr Stem Cell Res Ther. 12:476–483. 2017. View Article : Google Scholar : PubMed/NCBI

37 

Szade K, Zuba-Surma E, Rutkowski AJ, Jozkowicz A and Dulak J: CD45-CD14+CD34+ murine bone marrow low-adherent mesenchymal primitive cells preserve multilineage differentiation potential in long-term in vitro culture. Mol Cells. 31:497–507. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Baptista LS, do Amaral RJ, Carias RB, Aniceto M, Claudio-da-Silva C and Borojevic R: An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples. Cytotherapy. 11:706–715. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Healy C, Uwanogho D and Sharpe PT: Regulation and role of Sox9 in cartilage formation. Dev Dyn. 215:69–78. 1999. View Article : Google Scholar : PubMed/NCBI

40 

Somoza RA, Welter JF, Correa D and Caplan AI: Chondrogenic differentiation of mesenchymal stem cells: Challenges and unfulfilled expectations. Tissue Eng Part B Rev. 20:596–608. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Barry F and Murphy M: Mesenchymal stem cells in joint disease and repair. Nat Rev Rheumatol. 9:584–594. 2013. View Article : Google Scholar : PubMed/NCBI

42 

Jo A, Denduluri S, Zhang B, Wang Z, Yin L, Yan Z, Kang R, Shi LL, Mok J, Lee MJ and Haydon RC: The versatile functions of Sox9 in development, stem cells, and human diseases. Genes Dis. 1:149–161. 2014. View Article : Google Scholar : PubMed/NCBI

43 

Shim J and Nam JW: The expression and functional roles of microRNAs in stem cell differentiation. BMB Rep. 49:3–10. 2016. View Article : Google Scholar : PubMed/NCBI

44 

Martin EC, Qureshi AT, Llamas CB, Burow ME, King AG, Lee OC, Dasa V, Freitas MA, Forsberg JA, Elster EA, et al: Mirna biogenesis pathway is differentially regulated during adipose derived stromal/stem cell differentiation. Adipocyte. 7:96–105. 2018.PubMed/NCBI

45 

Zeng ZL, Lin XL, Tan LL, Liu YM, Qu K and Wang Z: MicroRNAs: Important regulators of induced pluripotent stem cell generation and differentiation. Stem Cell Rev. 14:71–81. 2018. View Article : Google Scholar

46 

Ran X, Xiao CH, Xiang GM and Ran XZ: Regulation of embryonic stem cell self-renewal and differentiation by MicroRNAs. Cell Reprogramm. 19:150–158. 2017. View Article : Google Scholar

47 

Papaioannou G, Mirzamohammadi F, Lisse TS, Nishimori S, Wein MN and Kobayashi T: MicroRNA-140 provides robustness to the regulation of hypertrophic chondrocyte differentiation by the PTHrP-HDAC4 pathway. J Bone Miner Res. 30:1044–1052. 2015. View Article : Google Scholar : PubMed/NCBI

48 

Zhang Y, Huang X and Yuan Y: MicroRNA-410 promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells through down-regulating Wnt3a. Am J Transl Res. 9:136–145. 2017.PubMed/NCBI

49 

Suchorska WM, Augustyniak E, Richter M and Trzeciak T: Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part A. Mol Med Rep. 15:2387–2401. 2017. View Article : Google Scholar : PubMed/NCBI

50 

Oh CD, Yasuda H, Zhao W, Henry SP, Zhang Z, Xue M, de Crombrugghe B and Chen D: SOX9 directly regulates CTGF/CCN2 transcription in growth plate chondrocytes and in nucleus pulposus cells of intervertebral disc. Sci Rep. 6:299162016. View Article : Google Scholar : PubMed/NCBI

51 

Maciejak A, Kostarska-Srokosz E, Gierlak W, Dluzniewski M, Kuch M, Marchel M, Opolski G, Kiliszek M, Matlak K, Dobrzycki S, et al: Circulating miR-30a-5p as a prognostic biomarker of left ventricular dysfunction after acute myocardial infarction. Sci Rep. 8:98832018. View Article : Google Scholar : PubMed/NCBI

52 

Zhang C, Ma X, Du J, Yao Z, Shi T, Ai Q, Chen X, Zhang Z, Zhang X and Yao X: MicroRNA-30a as a prognostic factor in urothelial carcinoma of bladder inhibits cellular malignancy by antagonising Notch1. BJU Int. 118:578–589. 2016. View Article : Google Scholar : PubMed/NCBI

53 

Perugorria MJ, Olaizola P, Labiano I, Esparza-Baquer A, Marzioni M, Marin JJG, Bujanda L and Banales JM: Wnt-β-catenin signalling in liver development, health and disease. Nat Rev Gastroenterol Hepatol. 16:121–136. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Wang Y, Yang G, Yu H, Zhou Z and Tang M: MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9. Exp Ther Med 18: 4689-4697, 2019.
APA
Zhang, H., Wang, Y., Yang, G., Yu, H., Zhou, Z., & Tang, M. (2019). MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9. Experimental and Therapeutic Medicine, 18, 4689-4697. https://doi.org/10.3892/etm.2019.8148
MLA
Zhang, H., Wang, Y., Yang, G., Yu, H., Zhou, Z., Tang, M."MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9". Experimental and Therapeutic Medicine 18.6 (2019): 4689-4697.
Chicago
Zhang, H., Wang, Y., Yang, G., Yu, H., Zhou, Z., Tang, M."MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4689-4697. https://doi.org/10.3892/etm.2019.8148
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Wang Y, Yang G, Yu H, Zhou Z and Tang M: MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9. Exp Ther Med 18: 4689-4697, 2019.
APA
Zhang, H., Wang, Y., Yang, G., Yu, H., Zhou, Z., & Tang, M. (2019). MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9. Experimental and Therapeutic Medicine, 18, 4689-4697. https://doi.org/10.3892/etm.2019.8148
MLA
Zhang, H., Wang, Y., Yang, G., Yu, H., Zhou, Z., Tang, M."MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9". Experimental and Therapeutic Medicine 18.6 (2019): 4689-4697.
Chicago
Zhang, H., Wang, Y., Yang, G., Yu, H., Zhou, Z., Tang, M."MicroRNA‑30a regulates chondrogenic differentiation of human bone marrow‑derived mesenchymal stem cells through targeting Sox9". Experimental and Therapeutic Medicine 18, no. 6 (2019): 4689-4697. https://doi.org/10.3892/etm.2019.8148
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