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Article

miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC

  • Authors:
    • Xiaowei Shen
    • Jiaqi Lei
    • Lei Du
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    Affiliations: Department of General Surgery, Zhongshan Hospital Qingpu Branch, Fudan University, Shanghai 200092, P.R. China, Department of General Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China
  • Pages: 375-383
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    Published online on: November 12, 2019
       https://doi.org/10.3892/etm.2019.8191
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Abstract

The limited efficacy of chemotherapy with Taxol (TAX) and cisplatin (DDP) in triple‑negative breast cancer (TNBC) has prompted the investigation of combined therapies. Previous studies demonstrated that microRNA (miR)‑31‑5p is involved in various biological processes. In the present study, it was hypothesized that the overexpression of miR‑31‑5p may enhance the efficacy of chemotherapy. The expression levels of miR‑31‑5p in the TNBC cell lines MDA‑MB‑231 and MDA‑MB‑468 were measured using reverse transcription‑quantitative PCR following transfection with miR‑31‑5p mimic or inhibitor. A Cell Counting Kit‑8 and flow cytometry assays suggested that the overexpression of miR‑31‑5p inhibited cell proliferation and promoted apoptosis, and these effects were reversed by transfecting a miR‑31‑5p inhibitor into MDA‑MB‑231 and MDA‑MB‑468 cells. Furthermore, the overexpression of miR‑31‑5p increased the sensitivity of cells to chemotherapy, which exhibited an increase in apoptosis and in the expression level of Bax, and a decrease in the expression level of Bcl‑2. Chemotherapy resistance induced by miR‑31‑5p inhibitor could be reversed by inhibiting the AKT signaling pathway in MDA‑MB‑231 and MDA‑MB‑468 cells. In conclusion, the present preclinical results indicated that targeting miR‑31‑5p may enhance the efficacy of TAX‑ and DDP‑mediated chemotherapy in TNBC.
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Copy and paste a formatted citation
Spandidos Publications style
Shen X, Lei J and Du L: miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC. Exp Ther Med 19: 375-383, 2020.
APA
Shen, X., Lei, J., & Du, L. (2020). miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC. Experimental and Therapeutic Medicine, 19, 375-383. https://doi.org/10.3892/etm.2019.8191
MLA
Shen, X., Lei, J., Du, L."miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC". Experimental and Therapeutic Medicine 19.1 (2020): 375-383.
Chicago
Shen, X., Lei, J., Du, L."miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC". Experimental and Therapeutic Medicine 19, no. 1 (2020): 375-383. https://doi.org/10.3892/etm.2019.8191
Copy and paste a formatted citation
x
Spandidos Publications style
Shen X, Lei J and Du L: miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC. Exp Ther Med 19: 375-383, 2020.
APA
Shen, X., Lei, J., & Du, L. (2020). miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC. Experimental and Therapeutic Medicine, 19, 375-383. https://doi.org/10.3892/etm.2019.8191
MLA
Shen, X., Lei, J., Du, L."miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC". Experimental and Therapeutic Medicine 19.1 (2020): 375-383.
Chicago
Shen, X., Lei, J., Du, L."miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC". Experimental and Therapeutic Medicine 19, no. 1 (2020): 375-383. https://doi.org/10.3892/etm.2019.8191
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