miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC

  • Authors:
    • Xiaowei Shen
    • Jiaqi Lei
    • Lei Du
  • View Affiliations

  • Published online on: November 12, 2019     https://doi.org/10.3892/etm.2019.8191
  • Pages: 375-383
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Abstract

The limited efficacy of chemotherapy with Taxol (TAX) and cisplatin (DDP) in triple‑negative breast cancer (TNBC) has prompted the investigation of combined therapies. Previous studies demonstrated that microRNA (miR)‑31‑5p is involved in various biological processes. In the present study, it was hypothesized that the overexpression of miR‑31‑5p may enhance the efficacy of chemotherapy. The expression levels of miR‑31‑5p in the TNBC cell lines MDA‑MB‑231 and MDA‑MB‑468 were measured using reverse transcription‑quantitative PCR following transfection with miR‑31‑5p mimic or inhibitor. A Cell Counting Kit‑8 and flow cytometry assays suggested that the overexpression of miR‑31‑5p inhibited cell proliferation and promoted apoptosis, and these effects were reversed by transfecting a miR‑31‑5p inhibitor into MDA‑MB‑231 and MDA‑MB‑468 cells. Furthermore, the overexpression of miR‑31‑5p increased the sensitivity of cells to chemotherapy, which exhibited an increase in apoptosis and in the expression level of Bax, and a decrease in the expression level of Bcl‑2. Chemotherapy resistance induced by miR‑31‑5p inhibitor could be reversed by inhibiting the AKT signaling pathway in MDA‑MB‑231 and MDA‑MB‑468 cells. In conclusion, the present preclinical results indicated that targeting miR‑31‑5p may enhance the efficacy of TAX‑ and DDP‑mediated chemotherapy in TNBC.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Shen X, Lei J and Du L: miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC. Exp Ther Med 19: 375-383, 2020
APA
Shen, X., Lei, J., & Du, L. (2020). miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC. Experimental and Therapeutic Medicine, 19, 375-383. https://doi.org/10.3892/etm.2019.8191
MLA
Shen, X., Lei, J., Du, L."miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC". Experimental and Therapeutic Medicine 19.1 (2020): 375-383.
Chicago
Shen, X., Lei, J., Du, L."miR‑31‑5p may enhance the efficacy of chemotherapy with Taxol and cisplatin in TNBC". Experimental and Therapeutic Medicine 19, no. 1 (2020): 375-383. https://doi.org/10.3892/etm.2019.8191