Effect of microRNA‑144‑5p on the proliferation, invasion and migration of human umbilical vein endothelial cells by targeting SMAD1

Fu,Wei; Liu,Zidong; Zhang,Jing; Shi,Yuxue; Zhao,Ruiyao; Zhao,Heng

doi:10.3892/etm.2019.8194

Effect of microRNA‑144‑5p on the proliferation, invasion and migration of human umbilical vein endothelial cells by targeting SMAD1

Authors:
- Wei Fu
- Zidong Liu
- Jing Zhang
- Yuxue Shi
- Ruiyao Zhao
- Heng Zhao
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Affiliations: Department of Cardiology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
Published online on: November 13, 2019 https://doi.org/10.3892/etm.2019.8194
Pages: 165-171
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Atherosclerosis is a multifactorial chronic disease that is a major cause of death and injury worldwide. Apoptosis of endothelial cells (ECs) serves an important role in the occurrence and development of atherosclerosis. MicroRNAs (miRNAs) serve a key role in atherosclerosis though regulating the function of ECs. At present, the role of miRNA‑144‑5p (miR‑144‑5p) in atherosclerosis is unclear. The aim of this study was to investigate the effect of miR‑144‑5p on atherosclerosis in oxidized low‑density lipoprotein (ox‑LDL)‑stimulated human umbilical vein endothelial cells (HUVECs).Results from the present study demonstrated that miR‑144‑5p overexpression could inhibit proliferation and induce apoptosis in HUVECs. To further study the biological function of miR‑144‑5p, the effects of modulating miR‑144‑5p expression on the invasion and migration of HUVECs were also examined. The results demonstrated that miR‑144‑5p upregulation suppressed HUVEC migration and invasion. TargetScan and dual luciferase reporter assay results demonstrated that SMAD1 was a direct target gene of miR‑144‑5p. miR‑144‑5p upregulation inhibited the expression of phosphorylated‑SMAD1/5/8 in the SMAD pathway. In conclusion, the data indicated that miR‑144‑5p serves an important role in the development of atherosclerosis through regulating the function of HUVECs by targeting SMAD1.

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