Open Access

MicroRNA‑138 inhibits tumor growth and enhances chemosensitivity in human cervical cancer by targeting H2AX

  • Authors:
    • Min Yuan
    • Shuting Zhao
    • Rui Chen
    • Guozeng Wang
    • Yachun Bie
    • Qianyu Wu
    • Jingxin Cheng
  • View Affiliations

  • Published online on: November 22, 2019     https://doi.org/10.3892/etm.2019.8238
  • Pages: 630-638
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNA‑138 (miR‑138) acts as a key regulator in the modulation of carcinogenesis in numerous tumor types. Chemoresistance is common and relevant to the failure of multiple treatment strategies for cervical cancer. However, the biological role of miR‑138 in the progression and chemosensitivity of cervical cancer is still unclear. The present study aimed to investigate the expression, function and mechanism of miR‑138 in cervical cancer. An miR‑138 mimic, inhibitor and negative control were transfected into SiHa and C33A cells. The expression of miR‑138 and its target were assessed by reverse transcription‑PCR, western blotting and immunohistochemistry. The functional significance of miR‑138 in tumor progression and chemosensitivity to cisplatin in vitro was examined by Cell Counting Kit‑8, flow cytometry, wound healing and Transwell assays. A tumor xenograft model was used to validate the effects in vivo. These results demonstrated that miR‑138 was significantly downregulated in cervical cancer cells. Overexpression of miR‑138 suppressed cervical cancer cell proliferation, invasion, increased apoptosis and enhanced chemotherapy sensitivity in vivo and in vitro. Furthermore, bioinformatics analysis and dual luciferase reporter assays demonstrated that H2AX served as a target for miR‑138, and the rescue experiment revealed that H2AX was a functional target of miR‑138. The protective effects of miR‑138 overexpression were dependent on H2AX. This study provides evidence that miR‑138/H2AX may be a novel therapeutic target in cervical cancer.
View Figures
View References

Related Articles

Journal Cover

January-2020
Volume 19 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yuan M, Zhao S, Chen R, Wang G, Bie Y, Wu Q and Cheng J: MicroRNA‑138 inhibits tumor growth and enhances chemosensitivity in human cervical cancer by targeting H2AX. Exp Ther Med 19: 630-638, 2020
APA
Yuan, M., Zhao, S., Chen, R., Wang, G., Bie, Y., Wu, Q., & Cheng, J. (2020). MicroRNA‑138 inhibits tumor growth and enhances chemosensitivity in human cervical cancer by targeting H2AX. Experimental and Therapeutic Medicine, 19, 630-638. https://doi.org/10.3892/etm.2019.8238
MLA
Yuan, M., Zhao, S., Chen, R., Wang, G., Bie, Y., Wu, Q., Cheng, J."MicroRNA‑138 inhibits tumor growth and enhances chemosensitivity in human cervical cancer by targeting H2AX". Experimental and Therapeutic Medicine 19.1 (2020): 630-638.
Chicago
Yuan, M., Zhao, S., Chen, R., Wang, G., Bie, Y., Wu, Q., Cheng, J."MicroRNA‑138 inhibits tumor growth and enhances chemosensitivity in human cervical cancer by targeting H2AX". Experimental and Therapeutic Medicine 19, no. 1 (2020): 630-638. https://doi.org/10.3892/etm.2019.8238