miR‑940 inhibits cell proliferation and promotes apoptosis in esophageal squamous cell carcinoma cells and is associated with post‑operative prognosis

Wang,Haiwen; Song,Tao; Qiao,Yanping; Sun,Jiangtao

doi:10.3892/etm.2019.8279

miR‑940 inhibits cell proliferation and promotes apoptosis in esophageal squamous cell carcinoma cells and is associated with post‑operative prognosis

Authors:
- Haiwen Wang
- Tao Song
- Yanping Qiao
- Jiangtao Sun
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Affiliations: Department of Cardio‑Thoracic Surgery, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China, Department of Endocrinology, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China, Department of Hematology, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China, Department of Oncology, Weihai Central Hospital, Weihai, Shandong 264400, P.R. China
Published online on: December 4, 2019 https://doi.org/10.3892/etm.2019.8279
Pages: 833-840
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to examine microRNA (miR)‑940 expression in esophageal squamous cell carcinoma (ESCC) tissues and cells, analyze its association with the clinicopathological features and prognosis of patients, and explore the potential underlying mechanisms. miR‑940 expression in ESCC cell lines and a normal esophageal cell line was detected using reverse transcription‑quantitative (RT‑q)PCR. Furthermore, 210 resected ESCC tissue and para‑carcinoma tissue specimens were collected, and miR‑940 expression in those tissues was detected by RT‑qPCR. In addition, the association of miR‑940 with the clinicopathological features and prognosis of patients was analyzed. In an in vitro experiment, miR‑940 mimics were transduced into ESCC cells by the liposome method. An MTT assay was used to detect the effect of miR‑940 on the viability of ESCC cells. The influence of miR‑940 on the cell cycle and apoptotic rate of ESCC cells was detected by flow cytometry. The present results indicated that the expression levels of miR‑940 in human ESCC tissues and cell lines were markedly downregulated, and that low expression of miR‑940 in ESCC tissues was significantly associated with a poor degree of differentiation, positive lymph node metastasis and advanced clinical stage. Kaplan‑Meier survival analysis suggested that low miR‑940 expression was associated with poor prognosis. Cox regression analysis revealed that lymph node metastasis, clinical stage and miR‑940 expression were independent risk factors affecting the prognosis of patients. Overexpression of miR‑940 in ESCC cells markedly reduced the cell viability, blocked the cell cycle at G0/G1 phase and promoted cell apoptosis. These results suggest that miR‑940 is downregulated in ESCC, which is linked to the occurrence and progression of ESCC. Conversely, overexpression of miR‑940 reduced the cell viability and promoted apoptosis of ESCC cells. Therefore, miR‑940 may be a promising novel prognostic marker and anti‑cancer target in ESCC.

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