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Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism

  • Authors:
    • Liya Zhang
    • Kunpeng Wu
    • Tao Bo
    • Lingyan Zhou
    • Ling Gao
    • Xiaoming Zhou
    • Wenbin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan, Shandong 250021, P.R. China, Scientific Center, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism, Shandong Academy of Clinical Medicine, Jinan, Shandong 250021, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 897-906
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    Published online on: December 4, 2019
       https://doi.org/10.3892/etm.2019.8281
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Abstract

Subclinical hypothyroidism (SCH) is becoming a global health problem due to its increasing prevalence and potential adverse effects, including cardiovascular diseases and nonalcoholic fatty liver disease (NAFLD). However, the association between SCH and NAFLD remains controversial. MicroRNAs (miRNAs/miRs) have been reported to be implicated in lipid metabolism disorders; however, how miRNAs regulate hepatic lipid metabolism in SCH mice remains unknown. The present study investigated miRNA alterations and proteome profiles in an SCH mouse model, which was generated by methimazole administration in mice for 16 weeks. Next, the profiles of 17 miRNAs that are critical to hepatic lipid metabolism and the proteome were investigated using reverse transcription‑quantitative polymerase chain reaction and iTRAQ labeling in the liver specimens of SCH (n=9) and control (n=7) mice. Putative target prediction of miRNAs was also conducted using TargetScan and miRanda. Compared with the control mice, SCH mice had 8 miRNAs and 36 proteins with significantly different expression in the liver tissues. Furthermore, a regulatory module containing 3 miRNAs (miR‑34a‑5p, miR‑24‑3p and miR‑130a‑3p) and 4 proteins (thioredoxin, selenium‑binding protein 2, elongation factor 1β and prosaposin) was identified. Overall, integrated analysis of miRNAs and the proteome highlighted a regulatory module between miRNAs and proteins, which, to a certain extent, may contribute to a better understanding of hepatic lipid metabolism disorders in SCH mice.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Wu K, Bo T, Zhou L, Gao L, Zhou X and Chen W: Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism. Exp Ther Med 19: 897-906, 2020.
APA
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., & Chen, W. (2020). Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism. Experimental and Therapeutic Medicine, 19, 897-906. https://doi.org/10.3892/etm.2019.8281
MLA
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., Chen, W."Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism". Experimental and Therapeutic Medicine 19.2 (2020): 897-906.
Chicago
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., Chen, W."Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism". Experimental and Therapeutic Medicine 19, no. 2 (2020): 897-906. https://doi.org/10.3892/etm.2019.8281
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Wu K, Bo T, Zhou L, Gao L, Zhou X and Chen W: Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism. Exp Ther Med 19: 897-906, 2020.
APA
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., & Chen, W. (2020). Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism. Experimental and Therapeutic Medicine, 19, 897-906. https://doi.org/10.3892/etm.2019.8281
MLA
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., Chen, W."Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism". Experimental and Therapeutic Medicine 19.2 (2020): 897-906.
Chicago
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., Chen, W."Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism". Experimental and Therapeutic Medicine 19, no. 2 (2020): 897-906. https://doi.org/10.3892/etm.2019.8281
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