Open Access

Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action

  • Authors:
    • Donglan Yuan
    • Xiaofang Zhang
    • Yinling Zhao
    • Hua Qian
    • Hezhu Wang
    • Cuiqin He
    • Xia Liu
    • Ting Guo
    • Mei Lin
    • Hong Yu
    • Jun Ye
  • View Affiliations

  • Published online on: December 4, 2019     https://doi.org/10.3892/etm.2019.8282
  • Pages: 965-971
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

This study aimed to elucidate the role of long non‑coding RNA activated by transforming growth factor‑β (lncRNA‑ATB) in ovarian cancer and its underlying mechanisms of action. Expression levels of lncRNA‑ATB in ovarian cancer cell line SKOV3 and in a healthy human ovarian cell line were compared using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The results indicated that lncRNA‑ATB was expressed at significantly higher levels in SKOV3 cells compared with the healthy cell line. After downregulation of lncRNA‑ATB expression in SKOV3 cells using lncRNA‑ATB‑short hairpin RNA, cell proliferation, apoptosis, invasion and migration were assessed using Cell counting kit‑8, Live Dead staining, Transwell assay and wound healing assay, respectively. RT‑qPCR and western blotting were used to quantify the expression of signal transducer and activator of transcription 3 (STAT3), phosphorylated (p)‑STAT3, and the additional epithelial to mesenchymal transition (EMT)‑related proteins E‑cadherin and vimentin in SKOV3 cells. LncRNA‑ATB downregulation significantly reduced SKOV3 cell proliferation, invasion and migration, promoted apoptosis, decreased the expression of p‑STAT3 and vimentin, and increased E‑cadherin expression. Taken together, these results suggest that lncRNA‑ATB downregulation can inhibit ovarian cancer cell proliferation, invasion and migration, and promote cell apoptosis. Lnc‑RNA‑ATB may therefore be a new target for ovarian cancer treatment.
View Figures
View References

Related Articles

Journal Cover

February-2020
Volume 19 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yuan D, Zhang X, Zhao Y, Qian H, Wang H, He C, Liu X, Guo T, Lin M, Yu H, Yu H, et al: Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action. Exp Ther Med 19: 965-971, 2020
APA
Yuan, D., Zhang, X., Zhao, Y., Qian, H., Wang, H., He, C. ... Ye, J. (2020). Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action. Experimental and Therapeutic Medicine, 19, 965-971. https://doi.org/10.3892/etm.2019.8282
MLA
Yuan, D., Zhang, X., Zhao, Y., Qian, H., Wang, H., He, C., Liu, X., Guo, T., Lin, M., Yu, H., Ye, J."Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action". Experimental and Therapeutic Medicine 19.2 (2020): 965-971.
Chicago
Yuan, D., Zhang, X., Zhao, Y., Qian, H., Wang, H., He, C., Liu, X., Guo, T., Lin, M., Yu, H., Ye, J."Role of lncRNA‑ATB in ovarian cancer and its mechanisms of action". Experimental and Therapeutic Medicine 19, no. 2 (2020): 965-971. https://doi.org/10.3892/etm.2019.8282