search button
Open Access

Differential expression profile study and gene function analysis of maternal foetal‑derived circRNA for screening for Down's syndrome

  • Authors:
    • Weiguo Sui
    • Qing Gan
    • Yan Chang
    • Minglin Ou
    • Jiejing Chen
    • Hua Lin
    • Wen Xue
    • Yan Wu
    • Huiyan He
    • Donge Tang
    • Yong Dai

  • View Affiliations

  • Published online on: December 5, 2019     https://doi.org/10.3892/etm.2019.8288
  • Pages: 1006-1016

  • Copyright: © Sui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recent studies have shown that circular RNAs (circRNAs) exhibit differential expression in certain diseases. However, to the best of our knowledge, maternal fetal‑derived circRNAs and mRNAs associated with Down's syndrome (DS) have not yet been investigated. A total of 12 umbilical cord blood samples were collected from pregnant women, including six women carrying fetuses with DS (diagnosed by G‑banding karyotype analysis), and six women carrying fetuses without DS. In addition, 12 peripheral blood samples were obtained from children, including six children with DS and six children without DS. Gene chip technology was used to screen for differentially expressed circRNAs and mRNAs in the cord blood samples, and were subsequently verified by reverse transcription‑quantitative polymerase chain reaction in peripheral blood from the children to identify potential biomarkers. Furthermore, circRNA/microRNA (miRNA) interactions were predicted using Arraystar miRNA target prediction software. There was a significant difference in the expression of hsa_circRNA_103127, hsa_circRNA_103112 and hsa_circRNA_104907 between cord blood obtained from the women carrying fetuses with and without DS, and between peripheral blood obtained from children with and without DS (P<0.01). As hsa_circRNA_103112 exhibited significant differences in expression between cord blood obtained from the women carrying fetuses with and without DS and between peripheral blood obtained from children with and without DS, its corresponding gene, ubiquitin specific peptidase 25, may be involved in the pathogenesis of the condition. These results suggested that hsa_circRNA_103112 may be upregulated in individuals with DS, resulting in an expression imbalance of diploid genes through interactions among circRNA, miRNA and mRNA. Therefore, the level of hsa_circRNA_103112 in the peripheral blood of a pregnant woman may serve as potential biomarker of fetal DS during non‑invasive prenatal screening.
View Figures
View References

Related Articles

Journal Cover

February-2020
Volume 19 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.7
  • Ranked Medicine Research and Experimental
    (total number of cites)
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Sui W, Gan Q, Chang Y, Ou M, Chen J, Lin H, Xue W, Wu Y, He H, Tang D, Tang D, et al: Differential expression profile study and gene function analysis of maternal foetal‑derived circRNA for screening for Down's syndrome. Exp Ther Med 19: 1006-1016, 2020
APA
Sui, W., Gan, Q., Chang, Y., Ou, M., Chen, J., Lin, H. ... Dai, Y. (2020). Differential expression profile study and gene function analysis of maternal foetal‑derived circRNA for screening for Down's syndrome. Experimental and Therapeutic Medicine, 19, 1006-1016. https://doi.org/10.3892/etm.2019.8288
MLA
Sui, W., Gan, Q., Chang, Y., Ou, M., Chen, J., Lin, H., Xue, W., Wu, Y., He, H., Tang, D., Dai, Y."Differential expression profile study and gene function analysis of maternal foetal‑derived circRNA for screening for Down's syndrome". Experimental and Therapeutic Medicine 19.2 (2020): 1006-1016.
Chicago
Sui, W., Gan, Q., Chang, Y., Ou, M., Chen, J., Lin, H., Xue, W., Wu, Y., He, H., Tang, D., Dai, Y."Differential expression profile study and gene function analysis of maternal foetal‑derived circRNA for screening for Down's syndrome". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1006-1016. https://doi.org/10.3892/etm.2019.8288