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Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling

  • Authors:
    • Zengtao Wang
    • Cui Wu
    • Min Zhang
    • Aiping Dong
    • Ruibin Niu
    • Jie Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Huashan Hospital‑North Fudan University, Shanghai 201907, P.R. China, Department of Anesthesiology, Central Hospital of Shanghai Yangpu District Affiliated to Tongji University, Shanghai 201907, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1336-1342
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    Published online on: December 12, 2019
       https://doi.org/10.3892/etm.2019.8319
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Abstract

The vascular endothelium plays an essential role in vascular disease and cardiovascular diseases. The effects and underlying mechanisms of sevoflurane on vascular endothelial growth factor (VEGF) in human endothelial cells have not been elucidated. The MTT colorimetric assay was used to determine HUVEC activity at different concentrations (1 and 3%, respectively) of sevoflurane for different time‑points (12, 24 and 48 h, respectively). The regulation of sevoflurane on the mRNA levels of VEGFa, VEGFb, VEGFc and VEGFR1, 2, 3 was analyzed by real‑time PCR. When VEGFR2 was inhibited by axitinib, VEGFR2 protein expression was determined by western blotting, and the cell viability was assessed by MTT analysis. The results revealed that sevoflurane increased cell viability in a dose‑ and time‑dependent manner. Sevoflurane significantly upregulated VEGFA mRNA expression only. In addition, sevoflurane increased the expression of VEGFR2 at the mRNA and protein levels, whereas sevoflurane did not modulate the mRNA expression of VEGFR1 and VEGFR3. Furthermore, sevoflurane failed to increase the mRNA and protein expression of VEGFR2 when VEGFR2 was inhibited by axitinib, an inhibitor of VEGF receptors. In conclusion, sevoflurane may be a promising agent against endothelium dysfunction‑caused vascular disease by activating the VEGF‑A/VEGFR2 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Wu C, Zhang M, Dong A, Niu R and Zhang J: Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling. Exp Ther Med 19: 1336-1342, 2020.
APA
Wang, Z., Wu, C., Zhang, M., Dong, A., Niu, R., & Zhang, J. (2020). Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling. Experimental and Therapeutic Medicine, 19, 1336-1342. https://doi.org/10.3892/etm.2019.8319
MLA
Wang, Z., Wu, C., Zhang, M., Dong, A., Niu, R., Zhang, J."Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling". Experimental and Therapeutic Medicine 19.2 (2020): 1336-1342.
Chicago
Wang, Z., Wu, C., Zhang, M., Dong, A., Niu, R., Zhang, J."Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1336-1342. https://doi.org/10.3892/etm.2019.8319
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Wu C, Zhang M, Dong A, Niu R and Zhang J: Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling. Exp Ther Med 19: 1336-1342, 2020.
APA
Wang, Z., Wu, C., Zhang, M., Dong, A., Niu, R., & Zhang, J. (2020). Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling. Experimental and Therapeutic Medicine, 19, 1336-1342. https://doi.org/10.3892/etm.2019.8319
MLA
Wang, Z., Wu, C., Zhang, M., Dong, A., Niu, R., Zhang, J."Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling". Experimental and Therapeutic Medicine 19.2 (2020): 1336-1342.
Chicago
Wang, Z., Wu, C., Zhang, M., Dong, A., Niu, R., Zhang, J."Sevoflurane promotes the proliferation of HUVECs by activating VEGF signaling". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1336-1342. https://doi.org/10.3892/etm.2019.8319
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