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Novel near‑infrared fluorescent probe for live cell imaging

  • Authors:
    • Meng Wan
    • Yubing Zhu
    • Jianjun Zou
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
    Copyright: © Wan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1213-1218
    |
    Published online on: December 13, 2019
       https://doi.org/10.3892/etm.2019.8323
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Abstract

Near infrared (NIR) fluorescent probes play a crucial role in biological system imaging. A novel NIR fluorescent probe, IR787, was designed in the present study. Compared with indocyanine green (ICG), IR787 showed lower background fluorescent interference and higher fluorescence enhancement. Fluorescence intensities were detected by a Cary Eclipse fluorescence spectrophotometer. The interference of intracellular ions (Cu2+, Ca2+, Mg2+ and Zn2+) on the measurement was negligible, which indicated a good photostability of IR787. MTT assay demonstrated that cell viability of human lung adenocarcinoma epithelial cell line A549 was not significantly affected by the use of the IR787 probe compared with the ICG probe. This result suggested that the IR787 probe was safe for in vitro cell imaging. In vitro NIR optical imaging experiments further revealed cellular uptake and strong intracellular NIR fluorescence of the IR787 probe in A549 cells. The excitation wavelength was 787 nm for IR787. Compared with the previously reported NIR fluorescent probe ICG, the IR787 NIR fluorescent probe had improved prospects for intracellular imaging. IR787 may play a pivotal role in the understanding cell biology, pharmacology and disease diagnosis.
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1 

Pansare VJ, Hejazi S, Faenza WJ and Prud'Homme RK: Review of long-wavelength optical and NIR imaging materials: Contrast agents, fluorophores and multifunctional nano carriers. Chem Mater. 24:812–827. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Chan M and Almutairi A: Nanogels as imaging agents for modalities spanning the electromagnetic spectrum. Mater Horiz. 3:21–40. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Galanzha EI, Shashkov EV, Tuchin VV and Zharov VP: In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes. Cytometry A. 73A:884–894. 2008. View Article : Google Scholar

4 

Dip F, Roy M, Lo Menzo E, Simpfendorfer C, Szomstein S and Rosenthal RJ: Routine use of fluorescent incisionless cholangiography as a new imaging modality during laparoscopic cholecystectomy. Surg Endosc. 29:1621–1626. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Kiviharju K, Salonen K, Moilanen U, Meskanen E, Leisola M and Eerikäinen T: On-line biomass measurements in bioreactor cultivations: Comparison study of two on-line probes. J Ind Microbiol Biotechnol. 34:561–566. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Zhang X, Gu YQ and Chen HY: Synthesis of biocompatible near infrared fluorescence Ag2S quantum dot and application in bioimaging. J Innov Optic Health Sci. 7:13500592014. View Article : Google Scholar

7 

Sampaio PN, Sales KC, Rosa FO, Lopes MB and Calado CR: High-throughput FTIR-based bioprocess analysis of recombinant cyprosin production. J Ind Microbiol Biotechnol. 44:49–61. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Yuan L, Lin W, Zhao S, Gao W, Chen B, He L and Zhu S: A unique approach to development of near-infrared fluorescent sensors for in vivo imaging. J Am Chem Soc. 134:13510–13523. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Jumarie C, Séïde M, Marcocci L, Pietrangeli P and Mateescu MA: Diamine oxidase from white pea (Lathyrus sativus) combined with catalase protects the human intestinal caco-2 cell line from histamine damage. Appl Biochem Biotechnol. 182:1171–1181. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Wang R and Yu C, Yu F, Chen L and Yu C: Molecular fluorescent probes for monitoring pH changes in living cells. TrAC Trends Anal Chem. 29:1004–1013. 2010. View Article : Google Scholar

11 

Luan LQ, Fang WJ, Liu W, Tian MG, Ni YX, Chen X, Yu XQ, He J, Yang Y and Li XG: 4-tert-butylphenoxy substituted phthalocyanine with RGD motif as highly selective one-photon and two-photon imaging probe for mitochondria and cancer cell. J Porphyrins Phthalocyanines. 20:1–10. 2016. View Article : Google Scholar

12 

Xing J, Zhou G, Sun C, Zhang H, Chen B, Zong X, Cai J and Ji M: Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging a549 cells. Biotechnol Lett. 38:1–6. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Kiyose K, Kojima H, Urano Y and Nagano T: Development of a ratiometric fluorescent zinc ion probe in near-infrared region, based on tricarbocyanine chromophore. J Am Chem Soc. 128:6548–6549. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Sun C, Cai J, Chen J, Wu Y, Wang P, Zhou G, Zong X, Chen B, Lv Y and Ji M: The synthesis of a novel near-infrared fluorescent probe and its application in imaging of living cells. Appl Biochem Biotechnol. 175:1644–1650. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Sun C, Wu Y, Cai J, Wang P, Zong X, Zhou G, Li L and Ji M: Synthesis of a near-infrared fluorescent probe and its application in imaging of MCF-7 cells. Biotechnol Lett. 36:1203–1207. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Liu K, Shang H, Meng F, Liu Y and Lin W: A novel near-infrared fluorescent platform with good photostability and the application for a reaction-based Cu (2+) probe in living cells. Talanta. 147:193–198. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Li C, Greenwood TR, Glunde K and Bhujwalla ZM: Synthesis and characterization of glucosamine-bound near-infrared probes for optical imaging. Org Lett. 8:3623–3626. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Luo S, Zhang E, Su Y, Cheng T and Shi C: A review of NIR dyes in cancer targeting and imaging. Biomaterials. 32:7127–7138. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Lim SY, Hong KH, Kim DI, Kwon H and Kim HJ: Tunable heptamethine-azo dye conjugate as an NIR fluorescent probe for the selective detection of mitochondrial glutathione over cysteine and homocysteine. J Am Chem Soc. 136:7018–7025. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Ziemiński K and Kowalskawentel M: Effect of different sugar beet pulp pretreatments on biogas production efficiency. Appl Biochem Biotechno. 181:1211–1227. 2017. View Article : Google Scholar

21 

Walther CG, Whitfield R and James DC: Importance of interaction between integrin and actin cytoskeleton in suspension adaptation of CHO cells. Appl Biochem Biotechnol. 178:1286–1302. 2016. View Article : Google Scholar : PubMed/NCBI

22 

El-Daly SM, Gamal-Eldeen AM, Abo-Zeid MA, Borai IH, Wafay HA and Abdel-Ghaffar AR: Photodynamic therapeutic activity of indocyanine green entrapped in polymeric nanoparticles. Photodiagnosis Photodyn Ther. 10:173–185. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Funayama T, Sakane M, Abe T, Hara I, Ozeki E and Ochiai N: Intraoperative near-infrared fluorescence imaging with novel indocyanine green-loaded nanocarrier for spinal metastasis: A preliminary animal study. Open Biomed Eng J. 6:80–84. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Shafirstein G, Bäumler W, Hennings LJ, Siegel ER, Ran F, Moreno MA, Webber J, Jackson C and Griffin RJ: Indocyanine green enhanced n ear infrared laser treatment of murine mammary carcinoma. Int J Cancer. 130:1208–1215. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Schubert GA, Barth M and Thomé C: The use of indocyanine green videography for intraoperative localization of intradural spinal tumors. Spine. 35:E212–E217. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Zhou LC, Zhao GJ, Liu JF, Han KL, Wu YK, Peng XJ and Sun MT: The charge transfer mechanism and spectral properties of a near-infrared heptamethine cyanine dye in alcoholic and aprotic solvents. J Photochem Photobiol Chem. 187:305–310. 2007. View Article : Google Scholar

27 

Tang B, Liu X, Xu K, Huang H, Yang G and An L: A dual near-infrared pH fluorescent probe and its application in imaging of HepG2 cells. Chem Commun (Camb). 41:3726–3728. 2007. View Article : Google Scholar

28 

Seidl K and Zinkernagel AS: The MTT assay is a rapid and reliable quantitative method to assess staphylococcus aureus induced endothelial cell damage. J Microbiol Methods. 92:307–309. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S and Boyd MR: New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 82:1107–1112. 1990. View Article : Google Scholar : PubMed/NCBI

30 

Funayama T, Sakane M, Abe T and Ochiai N: Photodynamic therapy with indocyanine green injection and near-infrared light irradiation has phototoxic effects and delays paralysis in spinal metastasis. Photomed Laser Surg. 30:47–53. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Liang Y, Gao W, Peng X, Deng X, Sun C and He B: Near infrared light responsive hybrid nanoparticles for synergistic therapy. Biomaterials. 100:76–90. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Nakayama A, Bianco AC, Zhang CY, Lowell BB and Frangioni JV: Quantitation of brown adipose tissue perfusion in transgenic mice using near-infrared fluorescence imaging. Mol Imaging. 2:37–49. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wan M, Zhu Y and Zou J: Novel near‑infrared fluorescent probe for live cell imaging. Exp Ther Med 19: 1213-1218, 2020.
APA
Wan, M., Zhu, Y., & Zou, J. (2020). Novel near‑infrared fluorescent probe for live cell imaging. Experimental and Therapeutic Medicine, 19, 1213-1218. https://doi.org/10.3892/etm.2019.8323
MLA
Wan, M., Zhu, Y., Zou, J."Novel near‑infrared fluorescent probe for live cell imaging". Experimental and Therapeutic Medicine 19.2 (2020): 1213-1218.
Chicago
Wan, M., Zhu, Y., Zou, J."Novel near‑infrared fluorescent probe for live cell imaging". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1213-1218. https://doi.org/10.3892/etm.2019.8323
Copy and paste a formatted citation
x
Spandidos Publications style
Wan M, Zhu Y and Zou J: Novel near‑infrared fluorescent probe for live cell imaging. Exp Ther Med 19: 1213-1218, 2020.
APA
Wan, M., Zhu, Y., & Zou, J. (2020). Novel near‑infrared fluorescent probe for live cell imaging. Experimental and Therapeutic Medicine, 19, 1213-1218. https://doi.org/10.3892/etm.2019.8323
MLA
Wan, M., Zhu, Y., Zou, J."Novel near‑infrared fluorescent probe for live cell imaging". Experimental and Therapeutic Medicine 19.2 (2020): 1213-1218.
Chicago
Wan, M., Zhu, Y., Zou, J."Novel near‑infrared fluorescent probe for live cell imaging". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1213-1218. https://doi.org/10.3892/etm.2019.8323
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