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Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway

  • Authors:
    • Jun Wang
    • Yupei Ke
    • Tao Shu
  • View Affiliations / Copyright

    Affiliations: Graduate School, Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Anorectal Surgery, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1297-1303
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    Published online on: December 16, 2019
       https://doi.org/10.3892/etm.2019.8329
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Abstract

The aim of the present study was to investigate changes in proliferation, apoptosis, inflammation and chemokine release of colon cancer cells after treatment with crocin, as well as to investigate the signaling pathway that is regulated by crocin. The inhibition rates of different doses of crocin on the proliferation of HCT116 cells were measured by MTT assay. The IC50 was calculated from the inhibition rates at 48 h. Proliferation curves of HCT116 cells were plotted after treatment with 271.18 µM (high‑dose group) or 135.6 µM (low‑dose group) crocin. Flow cytometry and Hoechst 33342/propidium iodide double staining were used for detecting apoptosis. ELISA was used to measure the levels of macrophage inflammatory protein 2, interleukin (IL)‑8, monocyte chemoattractant protein 1, tumor necrosis factor‑α, IL‑6 and IL‑1β in the supernatant from cultured HCT116 cells following both high‑ and low‑dose crocin treatment. Phosphorylated (P)‑STAT3/STAT3 in HCT116 cells were measured by western blotting. Crocin inhibited the proliferation of HCT116 cells in a dose‑dependent manner and the high‑dose treatment with crocin resulted in a lower rate of proliferation. Additionally, crocin increased the apoptosis of HCT116 cells and the high‑dose treatment with crocin led to a higher level of apoptosis. Notably, crocin decreased the secretion of chemokines and inflammatory factors from HCT116 cells and the high‑dose treatment with crocin caused the greatest reduction in secretion of the factors. Crocin reduced the ratio of P‑STAT3/STAT3, and thereby reduced the release of cytokines. The present study demonstrated that crocin may have pharmacological effects against the pathological behavior of colon cancer cells, and its mechanism of action may be related to the STAT3 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Ke Y and Shu T: Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway. Exp Ther Med 19: 1297-1303, 2020.
APA
Wang, J., Ke, Y., & Shu, T. (2020). Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway. Experimental and Therapeutic Medicine, 19, 1297-1303. https://doi.org/10.3892/etm.2019.8329
MLA
Wang, J., Ke, Y., Shu, T."Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway". Experimental and Therapeutic Medicine 19.2 (2020): 1297-1303.
Chicago
Wang, J., Ke, Y., Shu, T."Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1297-1303. https://doi.org/10.3892/etm.2019.8329
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Ke Y and Shu T: Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway. Exp Ther Med 19: 1297-1303, 2020.
APA
Wang, J., Ke, Y., & Shu, T. (2020). Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway. Experimental and Therapeutic Medicine, 19, 1297-1303. https://doi.org/10.3892/etm.2019.8329
MLA
Wang, J., Ke, Y., Shu, T."Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway". Experimental and Therapeutic Medicine 19.2 (2020): 1297-1303.
Chicago
Wang, J., Ke, Y., Shu, T."Crocin has pharmacological effects against the pathological behavior of colon cancer cells by interacting with the STAT3 signaling pathway". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1297-1303. https://doi.org/10.3892/etm.2019.8329
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