miR‑155 promotes fibroblast‑like synoviocyte proliferation and inflammatory cytokine secretion in rheumatoid arthritis by targeting FOXO3a

Wang,Yaxi; Feng,Tianying; Duan,Shasha; Shi,Yilu; Li,Shuling; Zhang,Xiaoshan; Zhang,Lei

doi:10.3892/etm.2019.8330

miR‑155 promotes fibroblast‑like synoviocyte proliferation and inflammatory cytokine secretion in rheumatoid arthritis by targeting FOXO3a

Authors:
- Yaxi Wang
- Tianying Feng
- Shasha Duan
- Yilu Shi
- Shuling Li
- Xiaoshan Zhang
- Lei Zhang
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Affiliations: Department of Ultrasound, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, P.R. China, Department of Medical Ultrasound, Bao'an Central Hospital of Shenzhen, Shenzhen, Guangdong 518102, P.R. China
Published online on: December 16, 2019 https://doi.org/10.3892/etm.2019.8330
Pages: 1288-1296
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to explore the expression and effects of microRNA (miR)‑155 in synovial fibroblasts of patients with rheumatoid arthritis (RA). A total of 89 synovial tissues from RA patients and 49 control synovial tissues were collected, and the levels of miR‑155 were measured by reverse transcription quantitative‑PCR and western blotting. Fibroblast‑like synoviocytes (FLS) were isolated from synovial tissues from the control group and were used to evaluate the roles of miR‑155 and forkhead box protein O3a (FOXO3a). MTT assay was used to measure the proliferation of FLS. The expression of miR‑155 in RA synovial tissues was significantly higher than that in the control group, but the expression of FOXO3a was significantly lower. In RA synovial tissues, miR‑155 expression was negatively correlated with FOXO3a expression, but was positively correlated with the release of inflammatory cytokines interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α (TNF‑α). A dual‑luciferase reporter system showed that miR‑155 inhibited the expression of FOXO3a in FLS cells. miR‑155 also promoted secretion of the inflammatory cytokines IL‑1β, IL‑6 and TNF‑α by FLS and proliferation of these cells by targeting FOXO3a.

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