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Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture

  • Authors:
    • Wen‑Lin Xiao
    • Guo Yu
    • Ning Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, P.R. China, School of Stomatology, Qingdao University, Qingdao, Shandong 266071, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1235-1242
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    Published online on: December 19, 2019
       https://doi.org/10.3892/etm.2019.8354
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Abstract

The aim of the present study was to improve methods for the suspension culture of mouse palatal shelves by comparing the expression of platelet‑derived growth factor receptor (PDGFR)‑α in palatal shelves in vivo, to that in vitro. The palatal shelves of C57BL/6 mouse embryos were obtained on gestation days (GDs) 13.5, 14.5, 15.0 and 15.5 for in vivo experiments. The palatal shelves were removed and observed under a stereomicroscope to investigate palatal development. For in vitro experiments, the palatal shelves were dissected under a stereomicroscope on GD 13.5 and then subjected to rotary culture for 0, 24, 36 or 48 h. The expression of PDGFR‑α at different time points was detected by immunohistochemical staining and western blot analysis. Both methods of analysis displayed PDGFR‑α expression in mesenchymal and epithelial cells at GD 13.5, 14.5, 15.0 and 15.5, in vivo and in vitro. The level of PDGFR‑α expression peaked on GD 14.5. The expression of PDGFR‑α in palatal shelves in in vitro rotary culture was consistent with that in vivo. Therefore, the novel technique of palatal rotary organ culture presented in the current study could provide a good model for studying the mechanism of pathological palatal fusion in vitro. Additionally, the present study further confirmed that PDGFR‑α gene expression was associated with the development of palatal shelves.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao WL, Yu G and Zhao N: Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture. Exp Ther Med 19: 1235-1242, 2020.
APA
Xiao, W., Yu, G., & Zhao, N. (2020). Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture. Experimental and Therapeutic Medicine, 19, 1235-1242. https://doi.org/10.3892/etm.2019.8354
MLA
Xiao, W., Yu, G., Zhao, N."Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture". Experimental and Therapeutic Medicine 19.2 (2020): 1235-1242.
Chicago
Xiao, W., Yu, G., Zhao, N."Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1235-1242. https://doi.org/10.3892/etm.2019.8354
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao WL, Yu G and Zhao N: Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture. Exp Ther Med 19: 1235-1242, 2020.
APA
Xiao, W., Yu, G., & Zhao, N. (2020). Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture. Experimental and Therapeutic Medicine, 19, 1235-1242. https://doi.org/10.3892/etm.2019.8354
MLA
Xiao, W., Yu, G., Zhao, N."Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture". Experimental and Therapeutic Medicine 19.2 (2020): 1235-1242.
Chicago
Xiao, W., Yu, G., Zhao, N."Development and gene expression of C57BL/6 mouse embryo palate shelves in rotary organ culture". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1235-1242. https://doi.org/10.3892/etm.2019.8354
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