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Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts

  • Authors:
    • Xian‑Yang Qin
    • Ting Su
    • Soichi Kojima
  • View Affiliations / Copyright

    Affiliations: Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, Wako, Saitama 351‑0198, Japan
    Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1522-1527
    |
    Published online on: December 27, 2019
       https://doi.org/10.3892/etm.2019.8384
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Abstract

Garlic and its sulfur constituents have numerous biological functions, such as antioxidant, anti‑inflammatory, anti‑microbial, anticancer, antidiabetic and cardioprotective effects. Fatty liver diseases, such as non‑alcoholic steatohepatitis, which is characterized by the accumulation of lipids and oxidative stress in hepatocytes and continual liver damage, has attracted much attention, and it is believed that it will become the leading etiology of liver cancer. We have previously reported that the growth‑suppressive effects of arachidonic acid (AA), an unsaturated fatty acid known to be a pro‑inflammatory precursor, is accompanied by the production of reactive oxygen species followed by the nuclear accumulation and activation of the protein crosslinking enzyme, transglutaminase (TG)2. In this study, we examined the potential role of garlic extracts in preventing the growth‑suppressive effects of AA on human hepatic cells. We also aimed to provide a mechanistic insight regarding the association between the hepatoprotective effects of garlic extract and the inhibition of the TG‑related crosslinking of nuclear proteins, which is not associated with hepatic lipid partitioning mediated by stearoyl‑CoA desaturase‑1. Given the critical roles of unsaturated fatty acids in the regulation of cancer cell stemness and immune surveillance in the context of chronic injury, we propose that garlic extracts may serve as a therapeutic option for the prevention of chronic liver injury and inflammation, as well as for the prevention of the carcinogenesis of fatty livers.
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1 

Banerjee SK, Mukherjee PK and Maulik SK: Garlic as an antioxidant: The good, the bad and the ugly. Phytotherapy research. PTR. 17:97–106. 2003.PubMed/NCBI

2 

Kyo E, Uda N, Kasuga S and Itakura Y: Immunomodulatory effects of aged garlic extract. J Nutr. 131 (Suppl 3):1075S–1079S. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Fratianni F, Riccardi R, Spigno P, Ombra MN, Cozzolino A, Tremonte P, Coppola R and Nazzaro F: Biochemical Characterization and Antimicrobial and Antifungal Activity of Two Endemic Varieties of Garlic (Allium sativum L.) of the Campania Region, Southern Italy. J Med Food. 19:686–691. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Xiao D, Pinto JT, Soh JW, Deguchi A, Gundersen GG, Palazzo AF, Yoon JT, Shirin H and Weinstein IB: Induction of apoptosis by the garlic-derived compound S-allylmercaptocysteine (SAMC) is associated with microtubule depolymerization and c-Jun NH(2)-terminal kinase 1 activation. Cancer Res. 63:6825–6837. 2003.PubMed/NCBI

5 

Ghyasi R, Mohaddes G and Naderi R: Combination effect of voluntary exercise and garlic (Allium sativum) on oxidative stress, cholesterol level and histopathology of heart tissue in type 1 diabetic rats. J Cardiovasc Thorac Res. 11:61–67. 2019. View Article : Google Scholar : PubMed/NCBI

6 

García-Villalón AL, Amor S, Monge L, Fernández N, Prodanov M, Muñoz M, Inarejos-García AM and Granado M: In vitro studies of an aged black garlic extract enriched in S-allylcysteine and polyphenols with cardioprotective effects. J Funct Foods. 27:189–200. 2016. View Article : Google Scholar

7 

Tsai JC, Chen YA, Wu JT, Cheng KC, Lai PS, Liu KF, Lin YK, Huang YT and Hsieh CW: Extracts from Fermented Black Garlic Exhibit a Hepatoprotective Effect on Acute Hepatic Injury. Molecules. 24(pii): E11122019. View Article : Google Scholar : PubMed/NCBI

8 

Wu ZR, Peng-Chen, Yang-Li, Li JY, Xin-Wang, Yong-Wang, Guo DD, Lei-Cui, Guan QG and Li HY: Two cinnamoyloctopamine antioxidants from garlic skin attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis. Phytomedicine. 22:178–182. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Xiao J, Xing F, Liu Y, Lv Y, Wang X, Ling M-T, Gao H, Ouyang S, Yang M, Zhu J, et al: Garlic-derived compound S-allylmercaptocysteine inhibits hepatocarcinogenesis through targeting LRP6/Wnt pathway. Acta Pharm Sin B. 8:575–586. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Panyod S, Wu WK, Ho CT, Lu KH, Liu CT, Chu YL, Lai YS, Chen WC, Lin YE, Lin SH and Sheen LY: Diet Supplementation with Allicin Protects against Alcoholic Fatty Liver Disease in Mice by Improving Anti-inflammation and Antioxidative Functions. J Agric Food Chem. 64:7104–7113. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Starley BQ, Calcagno CJ and Harrison SA: Nonalcoholic fatty liver disease and hepatocellular carcinoma: A weighty connection. Hepatology. 51:1820–1832. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Wong SW, Ting YW and Chan WK: Epidemiology of non-alcoholic fatty liver disease-related hepatocellular carcinoma and its implications. JGH Open. 2:235–241. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Qin XY, Tatsukawa H, Hitomi K, Shirakami Y, Ishibashi N, Shimizu M, Moriwaki H and Kojima S: Metabolome Analyses Uncovered a Novel Inhibitory Effect of Acyclic Retinoid on Aberrant Lipogenesis in a Mouse Diethylnitrosamine-Induced Hepatic Tumorigenesis Model. Cancer Prev Res (Phila). 9:205–214. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Qin XY, Suzuki H, Honda M, Okada H, Kaneko S, Inoue I, Ebisui E, Hashimoto K, Carninci P, Kanki K, et al: Prevention of hepatocellular carcinoma by targeting MYCN-positive liver cancer stem cells with acyclic retinoid. Proc Natl Acad Sci USA. 115:4969–4974. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Tatsukawa H, Fukaya Y, Frampton G, Martinez-Fuentes A, Suzuki K, Kuo TF, Nagatsuma K, Shimokado K, Okuno M, Wu J, et al: Role of transglutaminase 2 in liver injury via cross-linking and silencing of transcription factor Sp1. Gastroenterology. 136:1783–1795.e10. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Shrestha R, Shrestha R, Qin XY, Kuo TF, Oshima Y, Iwatani S, Teraoka R, Fujii K, Hara M, Li M, et al: Fungus-derived hydroxyl radicals kill hepatic cells by enhancing nuclear transglutaminase. Sci Rep. 7:47462017. View Article : Google Scholar : PubMed/NCBI

17 

Qin XY, Lu J, Cai M and Kojima S: Arachidonic acid suppresses hepatic cell growth through ROS-mediated activation of transglutaminase. FEBS Open Bio. 8:1703–1710. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Fujise K, Nagamori S, Hasumura S, Homma S, Sujino H, Matsuura T, Shimizu K, Niiya M, Kameda H, Fujita K, et al: Integration of hepatitis B virus DNA into cells of six established human hepatocellular carcinoma cell lines. Hepatogastroenterology. 37:457–460. 1990.PubMed/NCBI

19 

Qin XY, Wei F, Tanokura M, Ishibashi N, Shimizu M, Moriwaki H and Kojima S: The effect of acyclic retinoid on the metabolomic profiles of hepatocytes and hepatocellular carcinoma cells. PLoS One. 8:e828602013. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Rose P, Moore PK and Zhu YZ: Garlic and Gaseous Mediators. Trends Pharmacol Sci. 39:624–634. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Colín-González AL, Santana RA, Silva-Islas CA, Chánez-Cárdenas ME, Santamaria A and Maldonado PD: The antioxidant mechanisms underlying the aged garlic extract- and S-allylcysteine-induced protection. Oxid Med Cell Longev. 2012:9071622012. View Article : Google Scholar : PubMed/NCBI

23 

Kodai S, Takemura S, Minamiyama Y, Hai S, Yamamoto S, Kubo S, Yoshida Y, Niki E, Okada S, Hirohashi K, et al: S-allyl cysteine prevents CCl(4)-induced acute liver injury in rats. Free Radic Res. 41:489–497. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Eyster KM: The membrane and lipids as integral participants in signal transduction: Lipid signal transduction for the non-lipid biochemist. Adv Physiol Educ. 31:5–16. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Qin XY, Dohmae N and Kojima S: Reply to Yoshida: Liver cancer stem cells: Identification and lipid metabolic reprogramming. Proc Natl Acad Sci USA. 115:E6390–E6391. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Chen L, Ren J, Yang L, Li Y, Fu J, Li Y, Tian Y, Qiu F, Liu Z and Qiu Y: Stearoyl-CoA desaturase-1 mediated cell apoptosis in colorectal cancer by promoting ceramide synthesis. Sci Rep. 6:196652016. View Article : Google Scholar : PubMed/NCBI

27 

Lounis MA, Escoula Q, Veillette C, Bergeron KF, Ntambi JM and Mounier C: SCD1 deficiency protects mice against ethanol-induced liver injury. Biochim Biophys Acta. 1861:1662–1670. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Kurikawa N, Takagi T, Wakimoto S, Uto Y, Terashima H, Kono K, Ogata T and Ohsumi J: A novel inhibitor of stearoyl-CoA desaturase-1 attenuates hepatic lipid accumulation, liver injury and inflammation in model of nonalcoholic steatohepatitis. Biol Pharm Bull. 36:259–267. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Nardacci R, Lo Iacono O, Ciccosanti F, Falasca L, Addesso M, Amendola A, Antonucci G, Craxì A, Fimia GM, Iadevaia V, et al: Transglutaminase type II plays a protective role in hepatic injury. Am J Pathol. 162:1293–1303. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Piacentini M, Baiocchini A, Del Nonno F, Melino G, Barlev NA, Rossin F, D'Eletto M and Falasca L: Non-alcoholic fatty liver disease severity is modulated by transglutaminase type 2. Cell Death Dis. 9:2572018. View Article : Google Scholar : PubMed/NCBI

31 

Tatsukawa H, Furutani Y, Hitomi K and Kojima S: Transglutaminase 2 has opposing roles in the regulation of cellular functions as well as cell growth and death. Cell Death Dis. 7:e22442016. View Article : Google Scholar : PubMed/NCBI

32 

Shrestha R, Tatsukawa H, Shrestha R, Ishibashi N, Matsuura T, Kagechika H, Kose S, Hitomi K, Imamoto N and Kojima S: Molecular mechanism by which acyclic retinoid induces nuclear localization of transglutaminase 2 in human hepatocellular carcinoma cells. Cell Death Dis. 6:e20022015. View Article : Google Scholar : PubMed/NCBI

33 

Li J, Condello S, Thomes-Pepin J, Ma X, Xia Y, Hurley TD, Matei D and Cheng JX: Lipid Desaturation Is a Metabolic Marker and Therapeutic Target of Ovarian Cancer Stem Cells. Cell Stem Cell. 20:303–314.e5. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Ma C, Kesarwala AH, Eggert T, Medina-Echeverz J, Kleiner DE, Jin P, Stroncek DF, Terabe M, Kapoor V, ElGindi M, et al: NAFLD causes selective CD4(+) T lymphocyte loss and promotes hepatocarcinogenesis. Nature. 531:253–257. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Qin XY, Su T and Kojima S: Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts. Exp Ther Med 19: 1522-1527, 2020.
APA
Qin, X., Su, T., & Kojima, S. (2020). Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts. Experimental and Therapeutic Medicine, 19, 1522-1527. https://doi.org/10.3892/etm.2019.8384
MLA
Qin, X., Su, T., Kojima, S."Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts". Experimental and Therapeutic Medicine 19.2 (2020): 1522-1527.
Chicago
Qin, X., Su, T., Kojima, S."Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1522-1527. https://doi.org/10.3892/etm.2019.8384
Copy and paste a formatted citation
x
Spandidos Publications style
Qin XY, Su T and Kojima S: Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts. Exp Ther Med 19: 1522-1527, 2020.
APA
Qin, X., Su, T., & Kojima, S. (2020). Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts. Experimental and Therapeutic Medicine, 19, 1522-1527. https://doi.org/10.3892/etm.2019.8384
MLA
Qin, X., Su, T., Kojima, S."Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts". Experimental and Therapeutic Medicine 19.2 (2020): 1522-1527.
Chicago
Qin, X., Su, T., Kojima, S."Prevention of arachidonic acid‑induced liver injury by controlling oxidative stress‑mediated transglutaminase activation with garlic extracts". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1522-1527. https://doi.org/10.3892/etm.2019.8384
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