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Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways

  • Authors:
    • Wei Pan
    • Xiaoheng Xu
    • Yan Wang
    • Xingyu Song
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Experimental Center, Jilin Police College, Changchun, Jilin 130117, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1695-1700
    |
    Published online on: January 2, 2020
       https://doi.org/10.3892/etm.2020.8407
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Abstract

Acute lung injury (ALI) in children is a complex disease that is accompanied by an inflammatory response. The pathogenesis of ALI in children is not yet well understood. Mice with ALI exhibit inflammation of the lungs and decreased expression of interleukin (IL)‑35. To investigate whether the function of IL‑35 affects lipopolysaccharide (LPS)‑induced ALI, IL‑35 was overexpressed in cells. Enzyme‑linked immunosorbent assays indicated decreased levels of IL‑6 and tumor necrosis factor‑α in LPS‑induced and agomir‑IL‑35‑treated murine RAW264.7 macrophages. Finally, toll‑like receptor 4 (TLR4)/NF‑κB signaling pathways were analyzed. The expression of TLR4, NF‑κB p65 and NF‑κB p50 were decreased, as was the degradation of NF‑κB inhibitor‑α, in LPS‑induced and agomir‑IL‑35‑treated murine RAW264.7 macrophages. The results of the present study demonstrated that IL‑35 may exhibit a protective role in ALI by modulating the TLR4/NF‑κB signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Pan W, Xu X, Wang Y and Song X: Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways. Exp Ther Med 19: 1695-1700, 2020.
APA
Pan, W., Xu, X., Wang, Y., & Song, X. (2020). Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways. Experimental and Therapeutic Medicine, 19, 1695-1700. https://doi.org/10.3892/etm.2020.8407
MLA
Pan, W., Xu, X., Wang, Y., Song, X."Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways". Experimental and Therapeutic Medicine 19.3 (2020): 1695-1700.
Chicago
Pan, W., Xu, X., Wang, Y., Song, X."Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways". Experimental and Therapeutic Medicine 19, no. 3 (2020): 1695-1700. https://doi.org/10.3892/etm.2020.8407
Copy and paste a formatted citation
x
Spandidos Publications style
Pan W, Xu X, Wang Y and Song X: Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways. Exp Ther Med 19: 1695-1700, 2020.
APA
Pan, W., Xu, X., Wang, Y., & Song, X. (2020). Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways. Experimental and Therapeutic Medicine, 19, 1695-1700. https://doi.org/10.3892/etm.2020.8407
MLA
Pan, W., Xu, X., Wang, Y., Song, X."Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways". Experimental and Therapeutic Medicine 19.3 (2020): 1695-1700.
Chicago
Pan, W., Xu, X., Wang, Y., Song, X."Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways". Experimental and Therapeutic Medicine 19, no. 3 (2020): 1695-1700. https://doi.org/10.3892/etm.2020.8407
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