Open Access

Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways

  • Authors:
    • Wei Pan
    • Xiaoheng Xu
    • Yan Wang
    • Xingyu Song
  • View Affiliations

  • Published online on: January 2, 2020     https://doi.org/10.3892/etm.2020.8407
  • Pages: 1695-1700
  • Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute lung injury (ALI) in children is a complex disease that is accompanied by an inflammatory response. The pathogenesis of ALI in children is not yet well understood. Mice with ALI exhibit inflammation of the lungs and decreased expression of interleukin (IL)‑35. To investigate whether the function of IL‑35 affects lipopolysaccharide (LPS)‑induced ALI, IL‑35 was overexpressed in cells. Enzyme‑linked immunosorbent assays indicated decreased levels of IL‑6 and tumor necrosis factor‑α in LPS‑induced and agomir‑IL‑35‑treated murine RAW264.7 macrophages. Finally, toll‑like receptor 4 (TLR4)/NF‑κB signaling pathways were analyzed. The expression of TLR4, NF‑κB p65 and NF‑κB p50 were decreased, as was the degradation of NF‑κB inhibitor‑α, in LPS‑induced and agomir‑IL‑35‑treated murine RAW264.7 macrophages. The results of the present study demonstrated that IL‑35 may exhibit a protective role in ALI by modulating the TLR4/NF‑κB signaling pathways.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Pan, W., Xu, X., Wang, Y., & Song, X. (2020). Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways. Experimental and Therapeutic Medicine, 19, 1695-1700. https://doi.org/10.3892/etm.2020.8407
MLA
Pan, W., Xu, X., Wang, Y., Song, X."Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways". Experimental and Therapeutic Medicine 19.3 (2020): 1695-1700.
Chicago
Pan, W., Xu, X., Wang, Y., Song, X."Interleukin‑35 reduces inflammation in acute lung injury through inhibiting TLR4/NF‑κB signaling pathways". Experimental and Therapeutic Medicine 19, no. 3 (2020): 1695-1700. https://doi.org/10.3892/etm.2020.8407