Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
March-2020 Volume 19 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2020 Volume 19 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells

  • Authors:
    • Lei Dou
    • Qifang Yan
    • Deqin Yang
  • View Affiliations / Copyright

    Affiliations: Department of Conservative Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, P.R. China, Department of Endodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
  • Pages: 2377-2383
    |
    Published online on: January 10, 2020
       https://doi.org/10.3892/etm.2020.8444
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the effect of calcium hydroxide [Ca(OH)2], mineral trioxide aggregate (MTA), iRoot BP, platelet‑rich fibrin (PRF) and concentrated growth factors (CGF) on the proliferation, viability, apoptosis and mineralization of human dental pulp cells (HDPCs). HDPCs were treated with Ca(OH)2, MTA, iRoot BP, PRF and CGF exudates. Cell viability, apoptosis, proliferation, cell cycle and alkaline phosphatase (ALP) activity were evaluated in vitro. PRF significantly increased the cell proliferation as compared with that in the MTA and iRoot BP groups on day 3. The CGF group displayed higher proliferation rates as compared with that in the MTA group on days 3 and 7. The MTA group displayed the highest ALP activity on days 1 and 3, and the CGF group on day 7. Ca(OH)2 inhibited cell proliferation and the percentages of dead and apoptotic cells were relatively higher in the Ca(OH)2 group on days 1, 3 and 7 compared with those in the other groups. In conclusion, PRF and CGF may be potential pulp‑capping materials for vital pulp therapy. Future in vivo studies are required to confirm this.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Mente J, Hufnagel S, Leo M, Michel A, Gehrig H, Panagidis D, Saure D and Pfefferle T: Treatment outcome of mineral trioxide aggregate or calcium hydroxide direct pulp capping: Long-term results. J Endod. 40:1746–1751. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Parirokh M and Torabinejad M: Mineral trioxide aggregate: A comprehensive literature review-part I: Chemical, physical, and antibacterial properties. J Endod. 36:16–27. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Shi S, Bao ZF, Liu Y, Zhang DD, Chen X, Jiang LM and Zhong M: Comparison of in vivo dental pulp responses to capping with iRoot BP Plus and mineral trioxide aggregate. Int Endod J. 49:154–160. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Liu S, Wang S and Dong Y: Evaluation of a bioceramic as a pulp capping agent in vitro and in vivo. J Endod. 41:652–657. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Huang FM, Yang SF, Zhao JH and Chang YC: Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells. J Endod. 36:1628–1632. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Durmuşlar MC, Balli U, Dede FÖ, Misir AF, Bariş E, Kürkçü M and Kahraman SA: Histological evaluation of the effect of concentrated growth factor on bone healing. J Craniofac Surg. 27:1494–716. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Dou L, Yan Q, Liang P, Zhou P, Zhang Y and Ji P: iTRAQ-based proteomic analysis explore the influence of hypoxia on the proteome of dental pulp stem cells under 3D culture. Proteomics. 182018.

8 

Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, Nakata K, Uematsu K, Su CY and Kawase T: Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent. 2:192016. View Article : Google Scholar : PubMed/NCBI

9 

Takeda Y, Katsutoshi K, Matsuzaka K and Inoue T: The effect of concentrated growth factor on rat bone marrow cells in vitro and on calvarial bone healing in vivo. Int J Oral Maxillofac Implants. 30:1187–1196. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Isobe K, Watanebe T, Kawabata H, Kitamura Y, Okudera T, Okudera H, Uematsu K, Okuda K, Nakata K, Tanaka T and Kawase T: Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF). Int J Implant Dent. 3:172017. View Article : Google Scholar : PubMed/NCBI

11 

Chen YJ, Zhao YH, Zhao YJ, Liu NX, Lv X, Li Q, Chen FM and Zhang M: Potential dental pulp revascularization and odonto-/osteogenic capacity of a novel transplant combined with dental pulp stem cells and platelet-rich fibrin. Cell Tissue Res. 361:439–455. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Keswani D, Pandey RK, Ansari A and Gupta S: Comparative evaluation of platelet-rich fibrin and mineral trioxide aggregate as pulpotomy agents in permanent teeth with incomplete root development: A randomized controlled trial. J Endod. 40:599–605. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Hiremath H, Saikalyan S, Kulkarni SS and Hiremath V: Second-generation platelet concentrate (PRF) as a pulpotomy medicament in a permanent molar with pulpitis: A case report. Int Endod J. 45:105–112. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Zhang S, Yang X and Fan M: BioAggregate and iRoot BP Plus optimize the proliferation and mineralization ability of human dental pulp cells. Int Endod J. 46:923–929. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Öncel Torun Z, Torun D, Demirkaya K, Yavuz ST, Elçi MP, Sarper M and Avcu F: Effects of iRoot BP and white mineral trioxide aggregate on cell viability and the expression of genes associated with mineralization. Int Endod J. 48:986–993. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Qiu W, Sun B, He F and Zhang Y: MTA-induced Notch activation enhances the proliferation of human dental pulp cells by inhibiting autophagic flux. Int Endod J. 50 (Suppl 2):e52–e62. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Saeed MA, El-Rahman MA, Helal ME, Zaher AR and Grawish ME: Efficacy of human platelet rich fibrin exudate vs. fetal bovine serum on proliferation and differentiation of dental pulp stem cells. Int J Stem Cells. 10:38–47. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Qiao J, An N and Ouyang X: Quantification of growth factors in different platelet concentrates. Platelets. 28:774–778. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Luczaj-Cepowicz E, Marczuk-Kolada G, Pawinska M, Obidzinska M and Holownia A: Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials-an in vitro study. Folia Histochem Cytobiol. 55:86–93. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Li Z, Cao L, Fan M and Xu Q: Direct Pulp capping with calcium hydroxide or mineral trioxide aggregate: A meta-analysis. J Endod. 41:1412–1417. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Kundzina R, Stangvaltaite L, Eriksen HM and Kerosuo E: Capping carious exposures in adults: A randomized controlled trial investigating mineral trioxide aggregate versus calcium hydroxide. Int Endod J. 50:924–932. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Azimi S, Fazlyab M, Sadri D, Saghiri MA, Khosravanifard B and Asgary S: Comparison of pulp response to mineral trioxide aggregate and a bioceramic paste in partial pulpotomy of sound human premolars: A randomized controlled trial. Int Endod J. 47:873–881. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Wu T, Shi Z, Song H, Li Y and Li JH: Cytotoxicity of local anesthetics on rabbit adipose-derived mesenchymal stem cells during early chondrogenic differentiation. Exp Ther Med. 16:2843–2850. 2018.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Dou L, Yan Q and Yang D: Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells. Exp Ther Med 19: 2377-2383, 2020.
APA
Dou, L., Yan, Q., & Yang, D. (2020). Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells. Experimental and Therapeutic Medicine, 19, 2377-2383. https://doi.org/10.3892/etm.2020.8444
MLA
Dou, L., Yan, Q., Yang, D."Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells". Experimental and Therapeutic Medicine 19.3 (2020): 2377-2383.
Chicago
Dou, L., Yan, Q., Yang, D."Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2377-2383. https://doi.org/10.3892/etm.2020.8444
Copy and paste a formatted citation
x
Spandidos Publications style
Dou L, Yan Q and Yang D: Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells. Exp Ther Med 19: 2377-2383, 2020.
APA
Dou, L., Yan, Q., & Yang, D. (2020). Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells. Experimental and Therapeutic Medicine, 19, 2377-2383. https://doi.org/10.3892/etm.2020.8444
MLA
Dou, L., Yan, Q., Yang, D."Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells". Experimental and Therapeutic Medicine 19.3 (2020): 2377-2383.
Chicago
Dou, L., Yan, Q., Yang, D."Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2377-2383. https://doi.org/10.3892/etm.2020.8444
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team