Open Access

Luteolin induces mitochondrial apoptosis in HT29 cells by inhibiting the Nrf2/ARE signaling pathway

  • Authors:
    • Huan Yang
    • Bing‑Fang Liu
    • Fu‑Jia Xie
    • Wei‑Lin Yang
    • Nong Cao
  • View Affiliations

  • Published online on: January 22, 2020     https://doi.org/10.3892/etm.2020.8464
  • Pages: 2179-2187
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the current study was to investigate luteolin‑induced apoptosis and the molecular mechanisms underlying it in HT29 cells. A 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay was used to assess the cytotoxicity of luteolin on HT29 cells, and a dichloro‑dihydro‑fluorescein diacetate assay was used to measure cellular levels of reactive oxygen species (ROS). The effects of luteolin on the mitochondrial membrane potential were also evaluated. Bax and Bcl‑2 mRNA expression were determined using reverse transcription‑quantitative PCR. Additionally, western blot analysis was performed to assess changes in cytochrome c and caspase‑3 protein expression. Localization of nuclear factor erythroid 2‑related factor 2 (Nrf2) in the nucleus was also assessed using immunofluorescence. Luteolin exhibited cytotoxicity on HT29 cells in a time‑ and concentration‑dependent manner. Additionally, ROS production was indicated to be increased and ROS scavenging was decreased, which resulted in a significant increase in the levels of ROS in the cells. The mitochondrial membrane potential was indicated to decrease following luteolin treatment. At the molecular level, luteolin significantly increased the mRNA expression of Bax and the protein expression of cytochrome c, caspase‑3, p47phox and p22phox. The results revealed that luteolin decreased Bcl‑2 protein expression and inhibited the nuclear localization of Nrf2. In conclusion, the current study indicated that luteolin inhibited HT29 cell proliferation and induced apoptosis via the mitochondrial pathway.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Yang, H., Liu, B., Xie, F., Yang, W., & Cao, N. (2020). Luteolin induces mitochondrial apoptosis in HT29 cells by inhibiting the Nrf2/ARE signaling pathway. Experimental and Therapeutic Medicine, 19, 2179-2187. https://doi.org/10.3892/etm.2020.8464
MLA
Yang, H., Liu, B., Xie, F., Yang, W., Cao, N."Luteolin induces mitochondrial apoptosis in HT29 cells by inhibiting the Nrf2/ARE signaling pathway". Experimental and Therapeutic Medicine 19.3 (2020): 2179-2187.
Chicago
Yang, H., Liu, B., Xie, F., Yang, W., Cao, N."Luteolin induces mitochondrial apoptosis in HT29 cells by inhibiting the Nrf2/ARE signaling pathway". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2179-2187. https://doi.org/10.3892/etm.2020.8464