Open Access

CTRP3 induces an intermediate switch of CD14++CD16+ monocyte subset with anti‑inflammatory phenotype

  • Authors:
    • Hongtao Zhu
    • Yuan Ding
    • Youming Zhang
    • Xiaojun Ding
    • Jianfeng Zhao
    • Weili Ouyang
    • Junhui Gong
    • Yuqin Zou
    • Xueqing Liu
    • Weidong Wu
  • View Affiliations

  • Published online on: January 23, 2020     https://doi.org/10.3892/etm.2020.8467
  • Pages: 2243-2251
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Acute myocardial infarction (AMI) evokes a temporally coordinated immune response, in which monocytes are critically involved in the clearance of cell debris; however, excessive inflammation induced by the classical sub‑population of monocytes frequently limits the endogenous reparative process. In the present study, the potential of the anti‑inflammatory adipokine complement C1q tumor necrosis factor (TNF)‑related protein‑3 (CTRP3) to induce intermediate switch of monocytes to an anti‑inflammatory phenotype was explored. Circulating monocytes were isolated from patients with AMI at various time‑points (3‑5 h, 3 days and 7 days) and categorized by flow cytometry/immunostaining into three sub‑divisions based on the expression of CD14 and CD16 epitopes: Classical (CD14++/CD16‑), non‑classical (CD14+/CD16++) and intermediate populations (CD14++/CD16+). The phagocytic activity was evaluated by the ingestion of FITC‑Zymosan and 19F‑nanoemulsion and the migratory activity using Thin Cert™ Transwell assay. Monocytes were cultured using autologous serum in the presence of CTRP3 (1 µg/ml) for 24 h and the expression of interleukin 6 (IL‑6) and TNF‑α was quantified by reverse‑transcription quantitative PCR. In addition, SB203580, a p38 mitogen‑activated protein kinase (MAPK)/ERK inhibitor, was used to examine the downstream pathways of CTRP3. AMI evoked a transient increase in monocyte counts of the classical subset after onset of the ischemic insult, while the non‑classical and intermediate subsets persistently expanded (P<0.01). The monocytes from patients at 3 days after AMI displayed enhanced phagocytic and migratory activities in comparison with those from healthy volunteers (P<0.01). Of note, addition of CTRP3 induced an intermediate switch of monocyte subsets and antagonized the enhanced expression of cytokines, particularly IL‑6, in monocytes stressed by lipopolysaccharides, likely by blunting the ERK1/2 and P38 MAPK signaling pathway. In conclusion, the present study demonstrated a dynamic fluctuation of monocyte subsets and enhanced phagocytic and migratory activities in patients with AMI. Furthermore, the ‘proof‑of‑concept' evidence pinpoints CTRP3 as an alternative candidate to modulate the ‘uncontrolled' inflammatory response and thus to augment cardiac reparative processes in patients with AMI.
View Figures
View References

Related Articles

Journal Cover

March-2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu H, Ding Y, Zhang Y, Ding X, Zhao J, Ouyang W, Gong J, Zou Y, Liu X, Wu W, Wu W, et al: CTRP3 induces an intermediate switch of CD14++CD16+ monocyte subset with anti‑inflammatory phenotype. Exp Ther Med 19: 2243-2251, 2020
APA
Zhu, H., Ding, Y., Zhang, Y., Ding, X., Zhao, J., Ouyang, W. ... Wu, W. (2020). CTRP3 induces an intermediate switch of CD14++CD16+ monocyte subset with anti‑inflammatory phenotype. Experimental and Therapeutic Medicine, 19, 2243-2251. https://doi.org/10.3892/etm.2020.8467
MLA
Zhu, H., Ding, Y., Zhang, Y., Ding, X., Zhao, J., Ouyang, W., Gong, J., Zou, Y., Liu, X., Wu, W."CTRP3 induces an intermediate switch of CD14++CD16+ monocyte subset with anti‑inflammatory phenotype". Experimental and Therapeutic Medicine 19.3 (2020): 2243-2251.
Chicago
Zhu, H., Ding, Y., Zhang, Y., Ding, X., Zhao, J., Ouyang, W., Gong, J., Zou, Y., Liu, X., Wu, W."CTRP3 induces an intermediate switch of CD14++CD16+ monocyte subset with anti‑inflammatory phenotype". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2243-2251. https://doi.org/10.3892/etm.2020.8467