Open Access

Effects of hypoxic‑ischemic pre‑treatment on microvesicles derived from endothelial progenitor cells

  • Authors:
    • Wen Zeng
    • Qiaoling Lei
    • Jiao Ma
    • Rong Ju
  • View Affiliations

  • Published online on: January 23, 2020     https://doi.org/10.3892/etm.2020.8468
  • Pages: 2171-2178
  • Copyright: © Zeng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Endothelial progenitor cells (EPCs) have protective roles in ischemic injury due to their ability to improve endothelial function and modulate angiogenesis. Microvesicles (MVs) are small membrane particles released by various cell types, including EPCs, which affect various target cells by transferring carried genetic information, including microRNAs (miRNAs/miRs). Depending on the stimuli and cell types, MVs exert different functions. In the present study, oxygen‑glucose deprivation (OGD) was used to mimic ischemic‑hypoxic (HI) insult, where the effects of HI insult on EPC‑derived MVs (EPC‑MVs) were subsequently investigated. OGD induced Ca2+ influx in EPCs and increased the release of EPC‑MVs compared with normoxic conditions. In addition, MVs prepared from EPCs cultured under normoxic conditions or OGD conditions (OGD‑EMVs) had the ability to stimulate the proliferation of EPCs. Furthermore, OGD‑EMVs induced stronger effects on proliferation, which may be associated with the upregulation of miR‑210 in EPC‑MVs. In conclusion, the present results indicated that HI insult promoted the release of MVs from EPCs and upregulated miR‑210 in MVs, leading to positive modulation of the proliferation of EPCs cultured under normoxic conditions.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Zeng, W., Lei, Q., Ma, J., & Ju, R. (2020). Effects of hypoxic‑ischemic pre‑treatment on microvesicles derived from endothelial progenitor cells. Experimental and Therapeutic Medicine, 19, 2171-2178. https://doi.org/10.3892/etm.2020.8468
MLA
Zeng, W., Lei, Q., Ma, J., Ju, R."Effects of hypoxic‑ischemic pre‑treatment on microvesicles derived from endothelial progenitor cells". Experimental and Therapeutic Medicine 19.3 (2020): 2171-2178.
Chicago
Zeng, W., Lei, Q., Ma, J., Ju, R."Effects of hypoxic‑ischemic pre‑treatment on microvesicles derived from endothelial progenitor cells". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2171-2178. https://doi.org/10.3892/etm.2020.8468