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Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death

  • Authors:
    • Yang Gao
    • Xinran You
    • Yubo Liu
    • Fei Gao
    • Yuan Zhang
    • Jianrong Yang
    • Chen Yang
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Oral Disease, The Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Nuclear Medicine, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215002, P.R. China, Department of Orthopedics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu 215002, P.R. China, Department of Head and Neck Oncology, The Affiliated Jiangsu Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2202-2210
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    Published online on: January 28, 2020
       https://doi.org/10.3892/etm.2020.8475
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Abstract

The NOD‑like receptor protein 3/caspase‑1 inflammasome can be activated in human dental pulp tissue and fibroblasts; however, the underlying mechanisms are poorly understood. In the present study, lipopolysaccharide (LPS) was used to treat dental pulp cells to establish an inflammation model. Cell viability was examined by sulforhodamine B assay. Interleukin (IL)‑1β, caspase‑1, microtubule‑associated protein‑1 light chain 3‑II/I and p62 were determined by western blotting and ELISA. The phosphorylation (p‑) levels of NF‑κB and NF‑κB inhibitor (IκB)α protein were observed by western blotting. The results demonstrated that LPS induced pyroptotic cell death in cultured dental pulp cells, which was supported by the increased levels of IL‑1β, IL‑18 and caspase‑1. Rapamycin and 3‑methyladenine (3‑MA) were used to activate and inhibit autophagy, and it was observed that LPS increased autophagy and rapamycin reduced LPS‑induced dental pulp cell pyroptosis. However, 3‑MA aggravated LPS‑induced dental pulp cell pyroptosis. In addition, LPS inhibited the expression of IκBα, but increased the expression of p‑NF‑κB. Compared with the LPS group, 3‑MA further inhibited the expression of IκBα but promoted the expression of p‑NF‑κB. However, rapamycin produced the opposite results to LPS. Under LPS treatment, the NF‑κB pathway inhibitor BAY11‑7082 further enhanced the inhibitory effects of rapamycin, but inhibited the promoting effects of 3‑MA on the protein expression levels of IL‑1β and caspase‑1. The results of the present study demonstrated that there is an important crosstalk between autophagy, pyroptosis and the NF‑κB pathway, and that the modulation of pyroptosis in dental pulp cells may be a promising strategy to pulpitis therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, You X, Liu Y, Gao F, Zhang Y, Yang J and Yang C: Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death. Exp Ther Med 19: 2202-2210, 2020.
APA
Gao, Y., You, X., Liu, Y., Gao, F., Zhang, Y., Yang, J., & Yang, C. (2020). Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death. Experimental and Therapeutic Medicine, 19, 2202-2210. https://doi.org/10.3892/etm.2020.8475
MLA
Gao, Y., You, X., Liu, Y., Gao, F., Zhang, Y., Yang, J., Yang, C."Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death". Experimental and Therapeutic Medicine 19.3 (2020): 2202-2210.
Chicago
Gao, Y., You, X., Liu, Y., Gao, F., Zhang, Y., Yang, J., Yang, C."Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2202-2210. https://doi.org/10.3892/etm.2020.8475
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, You X, Liu Y, Gao F, Zhang Y, Yang J and Yang C: Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death. Exp Ther Med 19: 2202-2210, 2020.
APA
Gao, Y., You, X., Liu, Y., Gao, F., Zhang, Y., Yang, J., & Yang, C. (2020). Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death. Experimental and Therapeutic Medicine, 19, 2202-2210. https://doi.org/10.3892/etm.2020.8475
MLA
Gao, Y., You, X., Liu, Y., Gao, F., Zhang, Y., Yang, J., Yang, C."Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death". Experimental and Therapeutic Medicine 19.3 (2020): 2202-2210.
Chicago
Gao, Y., You, X., Liu, Y., Gao, F., Zhang, Y., Yang, J., Yang, C."Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell death". Experimental and Therapeutic Medicine 19, no. 3 (2020): 2202-2210. https://doi.org/10.3892/etm.2020.8475
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