Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL

  • Authors:
    • Ling Ding
    • Li Song
    • Weihong Zhao
    • Xiaoxue Li
    • Wen Gao
    • Zhuo Qi
    • Jintao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2457-2466
    |
    Published online on: February 7, 2020
       https://doi.org/10.3892/etm.2020.8496
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The current nested case‑control study was conducted to explore the prognostic value of cyclin‑dependent kinase inhibitor 2A (p16INK4a), marker of proliferation Ki‑67 (Ki‑67) and immunohistochemical cocktail containing antibodies directed against topoisomerase IIα (TOP2A) and minichromosome maintenance 2 (MCM2) proteins (ProExC) immuno‑qualitative features to predict low‑grade squamous intraepithelial lesion (LSIL) progression. A total of 92 LSIL patients were followed‑up for 2 years, where those with high‑grade squamous intraepithelial lesion (HSIL) or persistent LSIL were designated as the case group and those who spontaneously regressed were designated as the control group. The infection status of human papillomavirus (HPV) was evaluated using flow‑through hybridization and gene chip, whilst the expression of p16INK4a, Ki‑67 and ProExC were tested in LSIL patient biopsies by immunohistochemistry. All data were collected at the beginning of the follow‑up and patient outcomes were diagnosed by histopathological examination. To analyze the risk factors for LSIL progression, sensitivity, specificity, positive‑negative predictive value (PPV‑NPV), positive‑negative likelihood ratio (PLR‑NLR), Youden's index (YI) and multinomial logistic regression analysis was performed. The expression rates of p16INK4a, Ki‑67, and ProExC were found to be higher in the progression group compared with those in the persistence and regression groups. Only p16INK4a expression significantly associated with high‑risk HPV infection. With respect to predicting HSIL, p16INK4a staining was the most sensitive but Ki‑67 staining was found to be the most specific. YI was the highest (42.1%) for p16INK4a expression in the present study, followed by ProExC (39.5%) and Ki‑67 (28.3%). However, the expression of ProExC was found to be an independent risk factor for LSIL progression into HSIL. In conclusion, whilst immunohistochemical staining for p16INK4a, Ki‑67, and ProExC can be used to predict HSIL progression, only ProExC expression can be applied an independent risk factor for LSIL progression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016.PubMed/NCBI View Article : Google Scholar

2 

Wang Z, Wang J, Fan J, Zhao W, Yang X, Wu L, Li D, Ding L, Wang W, Xu J, et al: Risk factors for cervical intraepithelial neoplasia and cervical cancer in Chinese women: Large study in Jiexiu, Shanxi Province, China. J Cancer. 8:924–932. 2017.PubMed/NCBI View Article : Google Scholar

3 

Chen W, Zheng R, Zhang S, Zhao P, Zeng H, Zou X and He J: Annual report on status of cancer in China, 2010. Chin J Cancer Res. 26:48–58. 2014.PubMed/NCBI View Article : Google Scholar

4 

Schlecht NF, Platt RW, Duarte-Franco E, Costa MC, Sobrinho JP, Prado JC, Ferenczy A, Rohan TE, Villa LL and Franco EL: Human papillomavirus infection and time to progression and regression of cervical intraepithelial neoplasia. J Natl Cancer Inst. 95:1336–1343. 2003.PubMed/NCBI View Article : Google Scholar

5 

Pretet JL, Jacquard AC, Saunier M, Clavel C, Dachez R, Gondry J, Pradat P, Soubeyrand B, Leocmach Y, Mougin C, et al: Human papillomavirus genotype distribution in low-grade squamous intraepithelial lesions in France and comparison with CIN2-3 and invasive cervical cancer: The EDiTH III study. Gynecol Oncol. 110:179–184. 2008.PubMed/NCBI View Article : Google Scholar

6 

Koeneman MM, Kruitwagen RF, Nijman HW, Slangen BF, Van Gorp T and Kruse AJ: Natural history of high-grade cervical intraepithelial neoplasia: A review of prognostic biomarkers. Expert Rev Mol Diagn. 15:527–546. 2015.PubMed/NCBI View Article : Google Scholar

7 

Zhao WH, Hao M, Cheng XT, Yang X, Wang ZL, Cheng KY, Liu FL and Bai YX: c-myc gene copy number variation in cervical exfoliated cells detected on fluorescence in situ hybridization for cervical cancer screening. Gynecol Obstet Invest. 81:416–423. 2016.PubMed/NCBI View Article : Google Scholar

8 

Choi YJ and Park JS: Clinical significance of human papillomavirus genotyping. J Gynecol Oncol. 27(e21)2016.PubMed/NCBI View Article : Google Scholar

9 

Rodriguez AC, Schiffman M, Herrero R, Wacholder S, Hildesheim A, Castle PE, Solomon D and Burk R: Proyecto Epidemiologico Guanacaste G. Rapid clearance of human papillomavirus and implications for clinical focus on persistent infections. J Natl Cancer Inst. 100:513–517. 2008.PubMed/NCBI View Article : Google Scholar

10 

Egawa N, Egawa K, Griffin H and Doorbar J: Human papillomaviruses; epithelial tropisms, and the development of neoplasia. Viruses. 7:3863–3890. 2015.PubMed/NCBI View Article : Google Scholar

11 

Zhao W, Hao M, Wang Y, Feng N, Wang Z, Wang W, Wang J and Ding L: Association between folate status and cervical intraepithelial neoplasia. Eur J Clin Nutr. 70:837–842. 2016.PubMed/NCBI View Article : Google Scholar

12 

Walts AE and Bose S: p16, Ki-67, and BD ProExC immunostaining: A practical approach for diagnosis of cervical intraepithelial neoplasia. Hum Pathol. 40:957–964. 2009.PubMed/NCBI View Article : Google Scholar

13 

Ortega S, Malumbres M and Barbacid M: Cyclin D-dependent kinases INK4 inhibitors and cancer. Biochim Biophys Acta. 1602:73–87. 2002.PubMed/NCBI View Article : Google Scholar

14 

Takagi M, Sueishi M, Saiwaki T, Kametaka A and Yoneda Y: A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis. J Biol Chem. 276:25386–25391. 2001.PubMed/NCBI View Article : Google Scholar

15 

Leone G, DeGregori J, Yan Z, Jakoi L, Ishida S, Williams RS and Nevins JR: E2F3 activity is regulated during the cell cycle and is required for the induction of S phase. Genes Dev. 12:2120–2130. 1998.PubMed/NCBI View Article : Google Scholar

16 

Goodwin EC and DiMaio D: Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proc Natl Acad Sci USA. 97:12513–125138. 2000.PubMed/NCBI View Article : Google Scholar

17 

Halloush RA, Akpolat I, Jim Zhai Q, Schwartz MR and Mody DR: Comparison of ProEx C with p16INK4a and Ki-67 immunohistochemical staining of cell blocks prepared from residual liquid-based cervicovaginal material: A pilot study. Cancer. 114:474–480. 2008.PubMed/NCBI View Article : Google Scholar

18 

Badr RE, Walts AE, Chung F and Bose S: BD ProEx C: A sensitive and specific marker of HPV-associated squamous lesions of the cervix. Am J Surg Pathol. 32:899–906. 2008.PubMed/NCBI View Article : Google Scholar

19 

Liao GD, Sellors JW, Sun HK, Zhang X, Bao YP, Jeronimo J, Chen W, Zhao FH, Song Y, Cao Z, et al: p16 INK4A immunohistochemical staining and predictive value for progression of cervical intraepithelial neoplasia grade 1: A prospective study in China. Int J Cancer. 134:1715–1724. 2014.PubMed/NCBI View Article : Google Scholar

20 

Sagasta A, Castillo P, Saco A, Torné A, Esteve R, Marimon L, Ordi J and Del PM: p16 staining has limited value in predicting the outcome of histological low-grade squamous intraepithelial lesions of the cervix. Mod Pathol. 29:51–59. 2016.PubMed/NCBI View Article : Google Scholar

21 

Silva DC, Goncalves AK, Cobucci RN, Mendonca RC, Lima PH and Júnior GC: Immunohistochemical expression of P16, KI67 and P53 in cervical lesions-A systematic review. Pathol Res Pract. 213:723–729. 2017.PubMed/NCBI View Article : Google Scholar

22 

Solomon D, Davey D, Kurman R, Moriarty A, O'connor D, Prey M, Raab S, Sherman M, Wilbur D, Wright T Jr, et al: The 2001 bethesda system: Terminology for reporting results of cervical cytology. JAMA. 287:2114–2119. 2002.PubMed/NCBI View Article : Google Scholar

23 

Tao P, Zheng W, Wang Y and Bian ML: Sensitive HPV genotyping based on the flow-through hybridization and gene chip. J Biomed Biotechnol. 2012(938780)2012.PubMed/NCBI View Article : Google Scholar

24 

Bornstein J, Bentley J, Bösze P, Girardi F, Haefner H, Menton M, Perrotta M, Prendiville W, Russell P, Sideri M, et al: 2011 colposcopic terminology of the international federation for cervical pathology and colposcopy. Obstet Gynecol. 120:166–172. 2012.PubMed/NCBI View Article : Google Scholar

25 

Darragh TM, Colgan TJ, Thomas Cox J, Heller DS, Henry MR, Luff RD, McCalmont T, Nayar R, Palefsky JM, Stoler MH, et al: The Lower Anogenital Squamous Terminology Standardization project for HPV-associated lesions: Background and consensus recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology. Int J Gynecol Pathol. 32:76–115. 2013.PubMed/NCBI View Article : Google Scholar

26 

Wang WC, Wu TT, Chandan VS, Lohse CM and Zhang L: Ki-67 and ProExC are useful immnohistochemical markers in esophageal squamous intraepithelial neoplasia. Hum Pathol. 42:1430–1437. 2011.PubMed/NCBI View Article : Google Scholar

27 

Shi J, Liu H, Wilkerson M, Huang Y, Meschter S, Dupree W, Schuerch C and Lin F: Evaluation of p16INK4a, minichromosome maintenance protein 2, DNA topoisomerase IIalpha, ProEX C, and p16INK4a-ProEX C in cervical squamous intraepithelial lesions. Hum Pathol. 38:1335–1344. 2007.PubMed/NCBI View Article : Google Scholar

28 

Pinto AP, Schlecht NF, Woo TY, Crum CP and Cibas ES: Biomarker (ProEx C, p16(INK4A), and MiB-1) distinction of high-grade squamous intraepithelial lesion from its mimics. Mod Pathol. 21:1067–1074. 2008.PubMed/NCBI View Article : Google Scholar

29 

Liu C, Ding L, Bai L, Chen X, Kang H, Hou L and Wang J: Folate receptor alpha is associated with cervical carcinogenesis and regulates cervical cancer cells growth by activating ERK1-2-c-Fos-c-Jun. Biochem Biophys Res Commun. 491:1083–1089. 2017.PubMed/NCBI View Article : Google Scholar

30 

Galen RS and Gambino SR: Beyond Normality: The Predictive Value and Efficiency of Medical Diagnoses. John Wiley, Sons, New York, NY, pp1-237, 1975.

31 

Youden WJ: Index for rating diagnostic tests. Cancer. 3:32–35. 1950.PubMed/NCBI View Article : Google Scholar

32 

Khleif SN, DeGregori J, Yee CL, Otterson GA, Kaye FJ, Nevins JR and Howley PM: Inhibition of cyclin D-CDK4-CDK6 activity is associated with an E2F-mediated induction of cyclin kinase inhibitor activity. Proc Natl Acad Sci USA. 93:4350–4354. 1996.PubMed/NCBI View Article : Google Scholar

33 

Liu HQ, Wang YH, Wang LL and Hao M: P16INK4A and survivin: Diagnostic and prognostic markers in cervical intraepithelial neoplasia and cervical squamous cell carcinoma. Exp Mol Pathol. 99:44–49. 2015.PubMed/NCBI View Article : Google Scholar

34 

Branca M, Ciotti M, Santini D, Di Bonito L, Giorgi C, Benedetto A, Paba P, Favalli C, Costa S, Agarossi A, et al: p16(INK4A) Expression is related to grade of cin and high-risk human papillomavirus but does not predict virus clearance after conization or disease outcome. Int J Gynecol Pathol. 23:354–365. 2004.PubMed/NCBI View Article : Google Scholar

35 

Darragh TM, Colgan TJ, Cox JT, Heller DS, Henry MR, Luff RD, McCalmont T, Nayar R, Palefsky JM, Stoler MH, et al: The lower anogenital squamous terminology standardization project for HPV associated lesions: Background and consensus recommendations from the College of American Pathologists and the American society for colposcopy and cervical pathology. J Low Genit Tract Dis. 16:205–242. 2012.PubMed/NCBI View Article : Google Scholar

36 

Cortecchia S, Galanti G, Sgadari C, Costa S, De Lillo M, Caprara L, Barillari G, Monini P, Nannini R, Ensoli B and Bucchi L: Follow-up study of patients with cervical intraepithelial neoplasia grade 1 overexpressing p16Ink4a. Int J Gynecol Cancer. 23:1663–1669. 2013.PubMed/NCBI View Article : Google Scholar

37 

del Pino M, Garcia S, Fuste V, Alonso I, Fuste P, Torne A and Ordi J: Value of p16(INK4a) as a marker of progression-regression in cervical intraepithelial neoplasia grade 1. Am J Obstet Gynecol. 201:488.e1–7. 2009.PubMed/NCBI View Article : Google Scholar

38 

Hariri J and Oster A: The negative predictive value of p16INK4a to assess the outcome of cervical intraepithelial neoplasia 1 in the uterine cervix. Int J Gynecol Pathol. 26:223–228. 2007.PubMed/NCBI View Article : Google Scholar

39 

Ozaki S, Zen Y and Inoue M: Biomarker expression in cervical intraepithelial neoplasia: Potential progression predictive factors for low-grade lesions. Hum Pathol. 42:1007–1012. 2011.PubMed/NCBI View Article : Google Scholar

40 

Hanprasertpong J, Tungsinmunkong K, Chichareon S, Wootipoom V, Geater A, Buhachat R and Boonyapipat S: Correlation of p53 and Ki-67 (MIB-1) expressions with clinicopathological features and prognosis of early stage cervical squamous cell carcinomas. J Obstet Gynaecol Res. 36:572–580. 2010.PubMed/NCBI View Article : Google Scholar

41 

Mitildzans A, Arechvo A, Rezeberga D and Isajevs S: Expression of p63, p53 and Ki-67 in patients with cervical intraepithelial neoplasia. Turk Patoloji Derg. 33:9–16. 2017.PubMed/NCBI View Article : Google Scholar

42 

Nayar R and Wilbur DC: The Bethesda System for Reporting Cervical Cytology Definitions, Criteria, and Explanatory Note, 3rd (ed). Switzerland: Springer International Publishing, 103-134, 2015.

43 

Šekoranja D and Repše Fokter A: Triaging atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion With p16-Ki67 dual stain. J Low Genit Tract Dis. 21:108–111. 2017.PubMed/NCBI View Article : Google Scholar

44 

Tornesello ML, Buonaguro L, Giorgi-Rossi P and Buonaguro FM: Viral and cellular biomarkers in the diagnosis of cervical intraepithelial neoplasia and cancer. Biomed Res Int. 2013:519–619. 2013.PubMed/NCBI View Article : Google Scholar

45 

Kanthiya K, Khunnarong J, Tangjitgamol S, Puripat N and Tanvanich S: Expression of the p16 and Ki67 in cervical squamous intraepithelial lesions and cancer. Asian Pac J Cancer Prev. 17:3201–3202. 2016.PubMed/NCBI

46 

Kruse AJ, Baak JP, Janssen EA, Kjellevold KH, Fiane B, Lovslett K, Bergh J and Robboy S: Ki67 predicts progression in early CIN: Validation of a multivariate progression-risk model. Cell Oncol. 26:13–20. 2004.PubMed/NCBI View Article : Google Scholar

47 

Osaki M, Osaki M, Yamashita H, Shomori K, Yoshida H and Ito H: Expression of minichromosome maintenance-2 in human malignant fibrous histiocytomas: Correlations with Ki-67 and P53 expression, and apoptosis. Int J Mol Med. 10:161–168. 2002.PubMed/NCBI View Article : Google Scholar

48 

Gibbons D, Fogt F, Kasznica J, Holden J and Nikulasson S: Comparison of topoisomerase II alpha and MIB-1 expression in uterine cervical squamous lesions. Mod Pathol. 10:409–413. 1997.PubMed/NCBI

49 

Ishimi Y, Okayasu I, Kato C, Kwon HJ, Kimura H, Yamada K and Song SY: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix. Eur J Biochem. 270:1089–1101. 2003.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ding L, Song L, Zhao W, Li X, Gao W, Qi Z and Wang J: Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL. Exp Ther Med 19: 2457-2466, 2020.
APA
Ding, L., Song, L., Zhao, W., Li, X., Gao, W., Qi, Z., & Wang, J. (2020). Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL. Experimental and Therapeutic Medicine, 19, 2457-2466. https://doi.org/10.3892/etm.2020.8496
MLA
Ding, L., Song, L., Zhao, W., Li, X., Gao, W., Qi, Z., Wang, J."Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL". Experimental and Therapeutic Medicine 19.4 (2020): 2457-2466.
Chicago
Ding, L., Song, L., Zhao, W., Li, X., Gao, W., Qi, Z., Wang, J."Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2457-2466. https://doi.org/10.3892/etm.2020.8496
Copy and paste a formatted citation
x
Spandidos Publications style
Ding L, Song L, Zhao W, Li X, Gao W, Qi Z and Wang J: Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL. Exp Ther Med 19: 2457-2466, 2020.
APA
Ding, L., Song, L., Zhao, W., Li, X., Gao, W., Qi, Z., & Wang, J. (2020). Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL. Experimental and Therapeutic Medicine, 19, 2457-2466. https://doi.org/10.3892/etm.2020.8496
MLA
Ding, L., Song, L., Zhao, W., Li, X., Gao, W., Qi, Z., Wang, J."Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL". Experimental and Therapeutic Medicine 19.4 (2020): 2457-2466.
Chicago
Ding, L., Song, L., Zhao, W., Li, X., Gao, W., Qi, Z., Wang, J."Predictive value of p16INK4a, Ki‑67 and ProExC immuno‑qualitative features in LSIL progression into HSIL". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2457-2466. https://doi.org/10.3892/etm.2020.8496
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team