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MicroRNA‑24‑3p inhibition prevents cell growth of vascular smooth muscle cells by targeting Bcl‑2‑like protein 11

  • Authors:
    • Huanxin Zhang
    • Shizhen Xue
    • Yi Feng
    • Jun Shen
    • Jixian Zhao

  • View Affiliations

  • Published online on: February 11, 2020     https://doi.org/10.3892/etm.2020.8517
  • Pages: 2467-2474

  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Numerous reports have shown that dysfunction of vascular smooth muscle cells (VSMCs) serves a critical function in the development of cardiovascular disease, including coronary heart disease (CHD). microRNAs (miRNAs/miRs) have been reported to play important roles in regulating the function of VSMCs. The present study aimed to determine the role of miR‑24‑3p in VSMCs and to uncover the underlying mechanism. The expression of miR‑24‑3p in the peripheral blood samples of CHD patients was measured by reverse transcription‑quantitative (RT‑q)PCR. It was found that the level of miR‑24‑3p in the peripheral blood of patients with CHD was significantly upregulated compared with that in healthy controls. A dual luciferase reporter assay was performed to determine whether Bcl‑2‑like protein 11 (Bcl‑2L11) was a target gene of miR‑24‑3p, and it was identified that Bcl‑2L11 was a direct target of miR‑24‑3p. The mRNA level and protein expression of Bcl‑2L11 in the peripheral blood of patients with CHD were measured by RT‑qPCR and western blotting, respectively. The findings suggested that Bcl‑2L11 was downregulated in the peripheral blood of patients with CHD. In addition, it was found that downregulation of miR‑24‑3p suppressed VSMC proliferation and promoted VSMC apoptosis, while the effects of the miR‑24‑3p inhibitor on cell viability and apoptosis were reversed by Bcl‑2L11‑small interfering (si)RNA. Additionally, downregulation of miR‑24‑3p increased the levels of Bcl‑2L11, caspase‑3 and Bax, and decreased Bcl‑2 expression in VSMCs; these changes were abolished by Bcl‑2L11‑siRNA. In conclusion, the aforementioned results indicated that miR‑24‑3p was an important regulator in VSMC proliferation and apoptosis by targeting Bcl‑2L11, which suggested that miR‑24‑3p might be a potential therapeutic target for the treatment of CHD.
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April-2020
Volume 19 Issue 4

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Spandidos Publications style
Zhang H, Xue S, Feng Y, Shen J and Zhao J: MicroRNA‑24‑3p inhibition prevents cell growth of vascular smooth muscle cells by targeting Bcl‑2‑like protein 11. Exp Ther Med 19: 2467-2474, 2020
APA
Zhang, H., Xue, S., Feng, Y., Shen, J., & Zhao, J. (2020). MicroRNA‑24‑3p inhibition prevents cell growth of vascular smooth muscle cells by targeting Bcl‑2‑like protein 11. Experimental and Therapeutic Medicine, 19, 2467-2474. https://doi.org/10.3892/etm.2020.8517
MLA
Zhang, H., Xue, S., Feng, Y., Shen, J., Zhao, J."MicroRNA‑24‑3p inhibition prevents cell growth of vascular smooth muscle cells by targeting Bcl‑2‑like protein 11". Experimental and Therapeutic Medicine 19.4 (2020): 2467-2474.
Chicago
Zhang, H., Xue, S., Feng, Y., Shen, J., Zhao, J."MicroRNA‑24‑3p inhibition prevents cell growth of vascular smooth muscle cells by targeting Bcl‑2‑like protein 11". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2467-2474. https://doi.org/10.3892/etm.2020.8517