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Article

Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion

  • Authors:
    • Zhaorong Guo
    • Yanting Xu
    • Qiaoling Zheng
    • Yunyun Liu
    • Xiaoyan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Weihai Central Hospital Affiliated to Qingdao University, Weihai, Shandong 264400, P.R. China
  • Pages: 3159-3166
    |
    Published online on: February 21, 2020
       https://doi.org/10.3892/etm.2020.8537
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Abstract

The present study investigated the genetic etiology and possible immunological pathogenesis of recurrent spontaneous abortion by analyzing chromosome abnormalities, and the balance between T helper 17 (Th17) and regulatory T (Treg) cells. A total of 54 patients with recurrent spontaneous abortion were selected. The villus and decidual tissues, and peripheral venous blood were collected from each patient. Villus chromosome analysis was performed by high‑throughput gene sequencing. Flow cytometry was used to detect Th17 and Treg cells in patients without chromosome abnormalities (n=30) and the control group (normal pregnancy; n=32). Immunoglobulin (IG) combined with human chorionic gonadotropin hormone (HCG) treatment was given to patients without chromosome abnormalities (n=30). Changes in the expression levels of Th17 and Treg cells before and after treatment were compared with patients with successful pregnancy (n=18). Before treatment, compared with the control group, the proportion of Th17 cells in peripheral blood and decidual tissue was increased and the proportion of Treg cells decreased. After treatment, compared with patients before treatment, the proportion of Th17 cells decreased and Treg cells increased, and the Th17 and Treg cells balance was reversed with a biased towards Treg cells. The present results suggested that the Th17 and Treg cell immune imbalance may be an important immune factor in recurrent spontaneous abortion. IG combined with HCG therapy may improve pregnancy outcomes by reversing the imbalance between Th17 and Treg cells.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Z, Xu Y, Zheng Q, Liu Y and Liu X: Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion. Exp Ther Med 19: 3159-3166, 2020.
APA
Guo, Z., Xu, Y., Zheng, Q., Liu, Y., & Liu, X. (2020). Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion. Experimental and Therapeutic Medicine, 19, 3159-3166. https://doi.org/10.3892/etm.2020.8537
MLA
Guo, Z., Xu, Y., Zheng, Q., Liu, Y., Liu, X."Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion". Experimental and Therapeutic Medicine 19.4 (2020): 3159-3166.
Chicago
Guo, Z., Xu, Y., Zheng, Q., Liu, Y., Liu, X."Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3159-3166. https://doi.org/10.3892/etm.2020.8537
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Z, Xu Y, Zheng Q, Liu Y and Liu X: Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion. Exp Ther Med 19: 3159-3166, 2020.
APA
Guo, Z., Xu, Y., Zheng, Q., Liu, Y., & Liu, X. (2020). Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion. Experimental and Therapeutic Medicine, 19, 3159-3166. https://doi.org/10.3892/etm.2020.8537
MLA
Guo, Z., Xu, Y., Zheng, Q., Liu, Y., Liu, X."Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion". Experimental and Therapeutic Medicine 19.4 (2020): 3159-3166.
Chicago
Guo, Z., Xu, Y., Zheng, Q., Liu, Y., Liu, X."Analysis of chromosomes and the T helper 17 and regulatory T cell balance in patients with recurrent spontaneous abortion". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3159-3166. https://doi.org/10.3892/etm.2020.8537
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