Rhein‑8‑O‑β‑D‑glucopyranoside inhibited high glucose‑induced apoptosis of human mesangial cells by regulating the lincRNA ANRIL/let‑7a/TGF‑β1/Smad signaling pathway

Zhang,Lan‑Sheng; Li,Jing; Liu,Jia‑Ping

doi:10.3892/etm.2020.8544

Rhein‑8‑O‑β‑D‑glucopyranoside inhibited high glucose‑induced apoptosis of human mesangial cells by regulating the lincRNA ANRIL/let‑7a/TGF‑β1/Smad signaling pathway

Authors:
- Lan‑Sheng Zhang
- Jing Li
- Jia‑Ping Liu
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Affiliations: School of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, P.R. China
Published online on: February 24, 2020 https://doi.org/10.3892/etm.2020.8544
Pages: 2871-2878
Copyright : © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Diabetic nephropathy is one of most frequent complications of diabetes, and is the major cause of end‑stage disease in diabetic patients. The present study investigated the roles and mechanisms of Rhein‑8‑O‑β‑D‑glucopyranoside (Rg) protecting human mesangial cells (HMCs) from high glucose (HG)‑induced apoptosis. Using a Cell Counting Kit‑8 assay the proliferation of HMCs was analyzed, and flow cytometry was applied to detect apoptosis. The apoptosis‑associated protein Bcl‑2, caspase‑3 and members of the transforming growth factor‑β1 (TGF‑β1)/Smad signaling pathway were analyzed using a western blotting assay. HG significantly induced HMC apoptosis, and Rg markedly attenuated the HG‑induced apoptosis. HG decreased the Bcl‑2 expression and increased the caspase‑3 expression, and Rg treatment recovered the expressions of Bcl‑2 and caspase‑3 affected by HG. The underlying mechanisms were further analyzed, and it was demonstrated that HG significantly upregulated the long intervening non‑coding RNA (lincRNA) ANRIL expression level, downregulated let‑7a expression and activated the TGF‑β1/Smad signaling pathway; Rg treatment recovered the expressions of lincRNA ANRIL and let‑7a, and inhibited the TGF‑β1/Smad signaling pathway in the condition of HG. In conclusion, the present results suggested that Rg attenuated HG‑induced apoptosis of HMCs by regulating the lincRNA ANRIL/let‑7a/TGF‑β1/Smad signaling pathway.

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