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Article

Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model

  • Authors:
    • Ting Xue
    • Chun‑Li An
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology and Parasitology, College of Basic Medical Science, China Medical University, Shenyang, Liaoning 110122, P.R. China
  • Pages: 3133-3142
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    Published online on: February 24, 2020
       https://doi.org/10.3892/etm.2020.8545
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Abstract

Pneumocystis jirovecii (P. jirovecii), an opportunistic fungal pathogen, is the primary cause of Pneumocystis pneumonia (PCP), which affects immunocompromised individuals and leads to high morbidity and mortality. P. jirovecii colonization is associated with development of chronic obstructive pulmonary disease (COPD) in patients with HIV infection, and also non‑sufferers, and in primate models of HIV infection. However, the mechanisms underlying P. jirovecii infection in the pathogenesis of COPD have yet to be fully elucidated. To investigate the pathogenicity of P. jirovecii infection and its role in COPD development, the present study established a PCP rat model induced by dexamethasone sodium phosphate injection. Expression of COPD‑related biomarkers, including matrix metalloproteinases (MMPs) MMP‑2, MMP‑8, MMP‑9, and MMP‑12, and heat shock protein‑27 (HSP‑27), were quantified in the rat PCP model using reverse transcription‑quantitative polymerase chain reaction, ELISA, western blot analysis, immunohistochemistry and gelatin zymography. Body weight, COPD symptoms, and pulmonary histopathology were assessed. Inflammatory cell counts in splenic tissues were measured using flow cytometry. It was identified that MMP and HSP‑27 expression increased in the PCP rats, which was in agreement with previous literature. Therefore, it was hypothesized that P. jirovecii infection may have an important role in COPD development.
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Copy and paste a formatted citation
Spandidos Publications style
Xue T and An CL: Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model. Exp Ther Med 19: 3133-3142, 2020.
APA
Xue, T., & An, C. (2020). Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model. Experimental and Therapeutic Medicine, 19, 3133-3142. https://doi.org/10.3892/etm.2020.8545
MLA
Xue, T., An, C."Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model". Experimental and Therapeutic Medicine 19.4 (2020): 3133-3142.
Chicago
Xue, T., An, C."Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3133-3142. https://doi.org/10.3892/etm.2020.8545
Copy and paste a formatted citation
x
Spandidos Publications style
Xue T and An CL: Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model. Exp Ther Med 19: 3133-3142, 2020.
APA
Xue, T., & An, C. (2020). Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model. Experimental and Therapeutic Medicine, 19, 3133-3142. https://doi.org/10.3892/etm.2020.8545
MLA
Xue, T., An, C."Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model". Experimental and Therapeutic Medicine 19.4 (2020): 3133-3142.
Chicago
Xue, T., An, C."Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model". Experimental and Therapeutic Medicine 19, no. 4 (2020): 3133-3142. https://doi.org/10.3892/etm.2020.8545
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