Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2020 Volume 19 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis

  • Authors:
    • Chu Chen
    • Shengyu Cui
    • Weidong Li
    • Huricha Jin
    • Jianbo Fan
    • Yuyu Sun
    • Zhiming Cui
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2997-3008
    |
    Published online on: February 26, 2020
       https://doi.org/10.3892/etm.2020.8555
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Facet joint osteoarthritis (FJOA) is a common degenerative joint disorder with high prevalence in the elderly. FJOA causes lower back pain and lower extremity pain, and thus severely impacts the quality of life of affected patients. Emerging studies have focused on the histomorphological and histomorphometric changes in FJOA. However, the dynamic genetic changes in FJOA have remained to be clearly determined. In the present study, previously obtained RNA deep sequencing data were subjected to an ingenuity pathway analysis (IPA) and canonical signaling pathways of differentially expressed genes (DEGs) in FJOA were studied. The top 25 enriched canonical signaling pathways were identified and canonical signaling pathways with high absolute values of z‑scores, specifically leukocyte extravasation signaling, Tec kinase signaling and osteoarthritis pathway, were investigated in detail. DEGs were further categorized by disease, biological function and toxicity (tox) function. The genetic networks between DEGs as well as hub genes in these functional networks were also investigated. It was demonstrated that C‑X‑C motif chemokine ligand 8, elastase, neutrophil expressed, growth factor independent 1 transcriptional repressor, Spi‑1 proto‑oncogene, CCAAT enhancer binding protein epsilon, GATA binding protein 1, TAL bHLH transcription factor 1, erythroid differentiation factor, minichromosome maintenance complex component 4, BTG anti‑proliferation factor 2, BRCA1 DNA repair‑associated, cyclin D1, chromatin assembly factor 1 subunit A, triggering receptor expressed on myeloid cells 1 and tumor protein p63 were hub genes in the top 5 IPA networks (with a score >30). The present study provides insight into the pathological processes of FJOA from a genetic perspective and may thus benefit the clinical treatment of FJOA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Lewinnek GE and Warfield CA: Facet joint degeneration as a cause of low back pain. Clin Orthop Relat Res. 216–222. 1986.PubMed/NCBI

2 

Ko S, Vaccaro AR, Lee S, Lee J and Chang H: The prevalence of lumbar spine facet joint osteoarthritis and its association with low back pain in selected Korean populations. Clin Orthop Surg. 6:385–391. 2014.PubMed/NCBI View Article : Google Scholar

3 

Netzer C, Distel P, Wolfram U, Deyhle H, Jost GF, Schären S and Geurts J: Comparative analysis of bone structural parameters reveals subchondral cortical plate resorption and increased trabecular bone remodeling in human facet joint osteoarthritis. Int J Mol Sci. 19(pii: E845)2018.PubMed/NCBI View Article : Google Scholar

4 

Kalichman L, Li L, Kim DH, Guermazi A, Berkin V, O'Donnell CJ, Hoffmann U, Cole R and Hunter DJ: Facet joint osteoarthritis and low back pain in the community-based population. Spine (Phila Pa 1976). 33:2560–2565. 2008.PubMed/NCBI View Article : Google Scholar

5 

Kim JS, Ahmadinia K, Li X, Hamilton JL, Andrews S, Haralampus CA, Xiao G, Sohn HM, You JW, Seo YS, et al: Development of an experimental animal model for lower back pain by percutaneous injury-induced lumbar facet joint osteoarthritis. J Cell Physiol. 230:2837–2847. 2015.PubMed/NCBI View Article : Google Scholar

6 

Kim DS, Lee SJ, Park SY, Yoo HJ, Kim SH, Kim KJ and Cho HJ: Differentially expressed genes in rat dorsal root ganglia following peripheral nerve injury. Neuroreport. 12:3401–3405. 2001.PubMed/NCBI View Article : Google Scholar

7 

Netzer C, Urech K, Hügle T, Benz RM, Geurts J and Schären S: Characterization of subchondral bone histopathology of facet joint osteoarthritis in lumbar spinal stenosis. J Orthop Res. 34:1475–1480. 2016.PubMed/NCBI View Article : Google Scholar

8 

Bleil J, Maier R, Hempfing A, Schlichting U, Appel H, Sieper J and Syrbe U: Histomorphologic and histomorphometric characteristics of zygapophyseal joint remodeling in ankylosing spondylitis. Arthritis Rheumatol. 66:1745–1754. 2014.PubMed/NCBI View Article : Google Scholar

9 

Appel H, Maier R, Loddenkemper C, Kayser R, Meier O, Hempfing A and Sieper J: Immunohistochemical analysis of osteoblasts in zygapophyseal joints of patients with ankylosing spondylitis reveal repair mechanisms similar to osteoarthritis. J Rheumatol. 37:823–828. 2010.PubMed/NCBI View Article : Google Scholar

10 

Eisenstein SM and Parry CR: The lumbar facet arthrosis syndrome. Clinical presentation and articular surface changes. J Bone Joint Surg Br. 69:3–7. 1987.PubMed/NCBI

11 

Kim JS, Ali MH, Wydra F, Li X, Hamilton JL, An HS, Cs-Szabo G, Andrews S, Moric M, Xiao G, et al: Characterization of degenerative human facet joints and facet joint capsular tissues. Osteoarthritis Cartilage. 23:2242–2251. 2015.PubMed/NCBI View Article : Google Scholar

12 

Chen C, Bao GF, Xu G, Sun Y and Cui ZM: Altered Wnt and NF-κ signaling in facet joint osteoarthritis: Insights from RNA deep sequencing. Tohoku J Exp Med. 245:69–77. 2018.PubMed/NCBI View Article : Google Scholar

13 

Yu J, Gu X and Yi S: Ingenuity pathway analysis of gene expression profiles in distal nerve stump following nerve injury: Insights into wallerian degeneration. Front Cell Neurosci. 10(274)2016.PubMed/NCBI View Article : Google Scholar

14 

Yi S, Zhang H, Gong L, Wu J, Zha G, Zhou S, Gu X and Yu B: Deep sequencing and bioinformatic analysis of lesioned sciatic nerves after crush injury. PLoS One. 10(e0143491)2015.PubMed/NCBI View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

16 

Ghosh S, Dutta S, Thorne G, Boston A, Barfield A, Banerjee N, Walker R and Banerjee HN: Core canonical pathways involved in developing human glioblastoma multiforme (GBM). Int J Sci Res Sci Eng Technol. 3:458–465. 2017.PubMed/NCBI

17 

Raman S, FitzGerald U and Murphy JM: Interplay of inflammatory mediators with epigenetics and cartilage modifications in osteoarthritis. Front Bioeng Biotechnol. 6(22)2018.PubMed/NCBI View Article : Google Scholar

18 

Goldring MB and Goldring SR: Osteoarthritis. J Cell Physiol. 213:626–634. 2007.PubMed/NCBI

19 

Ray A and Ray BK: An inflammation-responsive transcription factor in the pathophysiology of osteoarthritis. Biorheology. 45:399–409. 2008.PubMed/NCBI

20 

Shen J, Abu-Amer Y, O'Keefe RJ and McAlinden A: Inflammation and epigenetic regulation in osteoarthritis. Connect Tissue Res. 58:49–63. 2017.PubMed/NCBI View Article : Google Scholar

21 

Fahy N, Farrell E, Ritter T, Ryan AE and Murphy JM: Immune modulation to improve tissue engineering outcomes for cartilage repair in the osteoarthritic joint. Tissue Eng Part B Rev. 21:55–66. 2015.PubMed/NCBI View Article : Google Scholar

22 

Wu D and Meydani SN: Age-associated changes in immune and inflammatory responses: Impact of vitamin E intervention. J Leukoc Biol. 84:900–914. 2008.PubMed/NCBI View Article : Google Scholar

23 

Meydani SN and Wu D: Nutrition and age-associated inflammation: Implications for disease prevention. JPEN J Parenter Enteral Nutr. 32:626–629. 2008.PubMed/NCBI View Article : Google Scholar

24 

Han SN and Meydani SN: Antioxidants, cytokines, and influenza infection in aged mice and elderly humans. J Infect Dis. 182 (Suppl 1)(S74-S80)2000.PubMed/NCBI View Article : Google Scholar

25 

Guo Y, Song Y, Zhang Y and Yang L: Identification of Btg2 As A mechanosensitive gene by functional screening integrative analyses. Mol Cell Biomech. 16 (Suppl 2)(S119)2019. View Article : Google Scholar

26 

Trzybulska D, Olewicz-Gawlik A, Graniczna K, Kisiel K, Moskal M, Cieślak D, Sikora J and Hrycaj P: Quantitative analysis of elastase and cathepsin G mRNA levels in peripheral blood CD14(+) cells from patients with rheumatoid arthritis. Cell Immunol. 292:40–44. 2014.PubMed/NCBI View Article : Google Scholar

27 

Dong S, Xia T, Wang L, Zhao Q and Tian J: Investigation of candidate genes for osteoarthritis based on gene expression profiles. Acta Orthop Traumatol Turc. 50:686–690. 2016.PubMed/NCBI View Article : Google Scholar

28 

Wang K, Zhao L, Liu X, Hao Z, Zhou Y, Yang C and Li H: Differential co-expression analysis of rheumatoid arthritis with microarray data. Mol Med Rep. 10:2421–2426. 2014.PubMed/NCBI View Article : Google Scholar

29 

Pellicelli M, Picard C, Wang D, Lavigne P and Moreau A: E2F1 and TFDP1 Regulate PITX1 expression in normal and osteoarthritic articular chondrocytes. PLoS One. 11(e0165951)2016.PubMed/NCBI View Article : Google Scholar

30 

Fei Q, Lin J, Meng H, Wang B, Yang Y, Wang Q, Su N, Li J and Li D: Identification of upstream regulators for synovial expression signature genes in osteoarthritis. Joint Bone Spine. 83:545–551. 2016.PubMed/NCBI View Article : Google Scholar

31 

Lambert C, Dubuc JE, Montell E, Vergés J, Munaut C, Noël A and Henrotin Y: Gene expression pattern of cells from inflamed and normal areas of osteoarthritis synovial membrane. Arthritis Rheumatol. 66:960–968. 2014.PubMed/NCBI View Article : Google Scholar

32 

Collins CE, La DT Yang HT, Massin F, Gibot S, Faure G and Stohl W: Elevated synovial expression of triggering receptor expressed on myeloid cells 1 in patients with septic arthritis or rheumatoid arthritis. Ann Rheum Dis. 68:1768–1774. 2009.PubMed/NCBI View Article : Google Scholar

33 

Tang J and Dong Q: Knockdown of TREM-1 suppresses IL-1β-induced chondrocyte injury via inhibiting the NF-κ pathway. Biochem Biophys Res Commun. 482:1240–1245. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen C, Cui S, Li W, Jin H, Fan J, Sun Y and Cui Z: Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis. Exp Ther Med 19: 2997-3008, 2020.
APA
Chen, C., Cui, S., Li, W., Jin, H., Fan, J., Sun, Y., & Cui, Z. (2020). Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis. Experimental and Therapeutic Medicine, 19, 2997-3008. https://doi.org/10.3892/etm.2020.8555
MLA
Chen, C., Cui, S., Li, W., Jin, H., Fan, J., Sun, Y., Cui, Z."Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis". Experimental and Therapeutic Medicine 19.4 (2020): 2997-3008.
Chicago
Chen, C., Cui, S., Li, W., Jin, H., Fan, J., Sun, Y., Cui, Z."Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2997-3008. https://doi.org/10.3892/etm.2020.8555
Copy and paste a formatted citation
x
Spandidos Publications style
Chen C, Cui S, Li W, Jin H, Fan J, Sun Y and Cui Z: Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis. Exp Ther Med 19: 2997-3008, 2020.
APA
Chen, C., Cui, S., Li, W., Jin, H., Fan, J., Sun, Y., & Cui, Z. (2020). Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis. Experimental and Therapeutic Medicine, 19, 2997-3008. https://doi.org/10.3892/etm.2020.8555
MLA
Chen, C., Cui, S., Li, W., Jin, H., Fan, J., Sun, Y., Cui, Z."Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis". Experimental and Therapeutic Medicine 19.4 (2020): 2997-3008.
Chicago
Chen, C., Cui, S., Li, W., Jin, H., Fan, J., Sun, Y., Cui, Z."Ingenuity pathway analysis of human facet joint tissues: Insight into facet joint osteoarthritis". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2997-3008. https://doi.org/10.3892/etm.2020.8555
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team