Association between microRNA machinery gene polymorphisms and recurrent implantation failure

Lee,Yubin; Ahn,Eun   Hee; Ryu,Chang   Soo; Kim,Jung  Oh; An,Hui  Jeong; Cho,Sung  Hwan; Kim,Ji  Hyang; Kim,Young  Ran; Lee,Woo  Sik; Kim,Nam  Keun

doi:10.3892/etm.2020.8556

Association between microRNA machinery gene polymorphisms and recurrent implantation failure

Authors:
- Yubin Lee
- Eun Hee Ahn
- Chang Soo Ryu
- Jung Oh Kim
- Hui Jeong An
- Sung Hwan Cho
- Ji Hyang Kim
- Young Ran Kim
- Woo Sik Lee
- Nam Keun Kim
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Affiliations: Department of Obstetrics and Gynecology, Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul 06135, Republic of Korea, Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, Gyeonggi 13497, Republic of Korea, Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi 13488, Republic of Korea
Published online on: February 26, 2020 https://doi.org/10.3892/etm.2020.8556
Pages: 3113-3123
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the potential association of five miRNA machinery gene polymorphisms (DICER1 rs3742330A>G, DROSHA rs10719T>C, RAN rs14035C>T, XPO5 rs11077A>C and DGCR8 rs417309G>A) with recurrent implantation failure (RIF), a clinical condition in which good‑quality embryos repeatedly fail to implant following two or more in vitro fertilization cycles, and its associated risk factors in Korean women. Therefore, the present study performed genotype analysis and assessed the frequency of these miRNA gene polymorphisms in patients diagnosed with RIF (n=119) and randomly selected controls (n=210) with at least one live birth and no history of pregnancy loss. The DROSHA rs10719T>C and RAN rs14035C>T polymorphisms were identified to be significantly associated with decreased prevalence of RIF. Additionally, the DROSHA rs10719 TC and the RAN rs14035 CT genotypes were present at significantly lower frequencies in the RIF group than in the control group (adjusted odds ratio=0.550; 95% CI, 0.339‑0.893; P=0.016; and adjusted odds ratio=0.590; 95% CI, 0.363‑0.958; P=0.033, respectively). Furthermore, the combined RAN rs14035 CT+TT genotype was observed to be associated with decreased RIF prevalence (adjusted odds ratio=0.616; 95% CI, 0.386‑0.982; P=0.042). Genotype combination analysis for the various miRNA polymorphisms revealed that the DROSHA TC genotype exhibited a highly significant negative association with RIF prevalence when combined with the RAN CT genotype (adjusted odds ratio=0.314; 95% CI, 0.147‑0.673; P=0.003; false discovery rate‑adjusted P=0.023). The present study revealed an association between the DROSHA rs10719 and RAN rs14035 3'UTR polymorphisms and decreased risk of RIF in Korean women, which suggests that these gene polymorphisms could represent potential markers for predicting RIF risk.

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