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Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS

  • Authors:
    • Su‑Hui Wu
    • Han‑Bing Li
    • Gen‑Lin Li
    • Ning Lv
    • Yue‑Juan Qi
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, Henan 450046, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3305-3315
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    Published online on: March 9, 2020
       https://doi.org/10.3892/etm.2020.8585
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Abstract

Cassia seed is the dried ripe seed of Cassia obtusifolia L. or Cassia tora L., which is widely used as a food or traditional Chinese medicine. The aim of the present study was to detect the components and metabolites in the culture of human or rat intestinal microflora suspension with the water decoction of cassia seed in vitro, using an ultra‑high‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry system equipped with a negative ion scan mode. Initially, ellagic acid was identified in the cassia seed decoction. Subsequently, six different metabolites, including urolithin (uro)‑A, uro‑B, uro‑D, uro‑M6, uro‑M7 and uro‑B‑glucuronide (glur), were detected after co‑culture of the cassia seed decoction with intestinal microflora, but not in the cassia seed decoction alone. Uro‑M6, uro‑M7, uro‑A and uro‑B were common metabolites in the culture of human or rat intestinal microflora suspension with the water decoction of cassia seed. However, uro‑D was only detected in the culture of rat intestinal microflora suspension with the water decoction of cassia seed, and uro‑B‑glur was only detected in the culture of human intestinal microflora with the water decoction of cassia seed. The uro and intermediate metabolites were produced by ellagic acid in the cassia seed decoction under the action of the intestinal microflora. The production of metabolites might be related to the abundance and diversity of the intestinal microflora in humans and rats. The present study provided rationale for further pharmacological and clinical studies on the mechanisms of action of cassia seeds.
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1 

Yang C, Yin X, Dong X Zhang X, You L, Wang W, Wang J, Chen Q and Ni J: Determination of the phytochemical composition of Jingning fang and the in vivo pharmacokinetics of its metabolites in rat plasma by UPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 1067:71–88. 2017.PubMed/NCBI View Article : Google Scholar

2 

Wu S, Xu W, Wang FR and Yang XW: Study of the biotransformation of tongmai formula by human intestinal flora and its intestinal permeability across the caco-2 cell monolayer. Molecules. 20:18704–18716. 2015.PubMed/NCBI View Article : Google Scholar

3 

Feng W, Ao H, Peng C and Yan D: Gut microbiota, a new frontier to understand traditional Chinese medicines. Pharmacol Res. 142:176–191. 2019.PubMed/NCBI View Article : Google Scholar

4 

Goodman AL and Gordon JI: Our unindicted coconspirators: Human metabolism from a microbial perspective. Cell Metab. 12:111–116. 2010.PubMed/NCBI View Article : Google Scholar

5 

Wang RF, Yuan M, Yang XB, Xu W and Yang XW: Intestinal bacterial transformation-a nonnegligible part of Chinese medicine research. J Asian Nat Prod Res. 15:532–549. 2013.PubMed/NCBI View Article : Google Scholar

6 

Koeth RA, Wang Z, Levison BS, Buffa JA, Org E, Sheehy BT, Britt EB, Fu X, Wu Y, Li L, et al: Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis. Nat Med. 19:576–585. 2013.PubMed/NCBI View Article : Google Scholar

7 

Cohen LJ, Esterhazy D, Kim SH, Lemetre C, Aguilar RR, Gordon EA, Pickard AJ, Cross JR, Emiliano AB, Han SM, et al: Commensal bacteria make GPCR ligands that mimic human signalling molecules. Nature. 549:48–53. 2017.PubMed/NCBI View Article : Google Scholar

8 

State Pharmacopoeia Commission of the PRC: Pharmacopoeia of the People's Republic of China. Vol. Ⅰ. Beijing. People's Medical Publishing House, 2015.

9 

Xin T, Zhang Y, Pu X, Gao R, Xu Z and Song J: Trends in herbgenomics. Sci China Life Sci. 62:288–308. 2019.PubMed/NCBI View Article : Google Scholar

10 

Huang YL, Chow CJ and Tsai YH: Composition, characteristics, and in-vitro physiological effects of the water-soluble polysaccharides from Cassia seed. Food Chem. 134:1967–1972. 2012.PubMed/NCBI View Article : Google Scholar

11 

Sahu J, Koley KM and Sahu BD: Attribution of antibacterial and antioxidant activity of Cassia tora extract toward its growth promoting effect in broiler birds. Vet World. 10:221–226. 2017.PubMed/NCBI View Article : Google Scholar

12 

Dong X, Fu J, Yin X, Yang C, Zhang X, Wang W, Du X, Wang Q and Ni J: Cassiae semen: A review of its phytochemistry and pharmacology (Review). Mol Med Rep. 16:2331–2346. 2017.PubMed/NCBI View Article : Google Scholar

13 

Xie Q, Guo FF and Zhou W: Protective effects of cassia seed ethanol extract against carbon tetrachloride-induced liver injury in mice. Acta Biochim Pol. 59:265–270. 2012.PubMed/NCBI

14 

Kim M, Lim SJ, Lee HJ and Nho CW: Cassia tora seed extract and its active compound aurantio-obtusin inhibit allergic responses in IgE-mediated mast cells and anaphylactic models. J Agric Food Chem. 63:9037–9046. 2015.PubMed/NCBI View Article : Google Scholar

15 

Yi JH, Park HJ, Lee S, Jung JW, Kim BC, Lee YC, Ryu JH and Kim DH: Cassia obtusifolia seed ameliorates amyloid β-induced synaptic dysfunction through anti-inflammatory and Akt/GSK-3β pathways. J Ethnopharmacol. 178:50–57. 2016.PubMed/NCBI View Article : Google Scholar

16 

Ju MS, Kim HG, Choi JG, Ryu JH, Hur J, Kim YJ and Oh MS: Cassiae semen, a seed of Cassia obtusifolia, has neuroprotective effects in Parkinson's disease models. Food Chem Toxicol. 48:2037–2044. 2010.PubMed/NCBI View Article : Google Scholar

17 

Drever BD, Anderson WG, Riedel G, Kim DH, Ryu JH, Choi DY and Platt B: The seed extract of Cassia obtusifolia offers neuroprotection to mouse hippocampal cultures. J Pharmacol Sci. 107:380–392. 2008.PubMed/NCBI View Article : Google Scholar

18 

Shi BJ, Zhang WD, Jiang HF, Zhu YY, Chen L, Zha XM, Lu YY and Zhang WM: A new anthraquinone from seed of Cassia obtusifolia. Nat Prod Res. 30:35–41. 2016.PubMed/NCBI View Article : Google Scholar

19 

Xu YL, Tang LY, Zhou XD, Zhou GH and Wang ZJ: Five new anthraquinones from the seed of Cassia obtusifolia. Arch Pharm Res. 38:1054–1058. 2015.PubMed/NCBI View Article : Google Scholar

20 

García-Villalba R, Beltrán D, Espín JC, Selma MV and Tomás-Barberán FA: Time course production of urolithins from ellagic acid by human gut microbiota. J Agric Food Chem. 61:8797–8806. 2013.PubMed/NCBI View Article : Google Scholar

21 

Koppel N, Maini Rekdal V and Balskus EP: Chemical transformation of xenobiotics by the human gut microbiota. Science. 356:2017.PubMed/NCBI View Article : Google Scholar

22 

Mullen W, Yokota T, Lean ME and Crozier A: Analysis of ellagitannins and conjugates of ellagic acid and quercetin in raspberry fruits by LC-MSn. Phytochemistry. 64:617–624. 2003.PubMed/NCBI View Article : Google Scholar

23 

Lee JH, Johnson JV and Talcott ST: Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS. J Agric Food Chem. 53:6003–6010. 2005.PubMed/NCBI View Article : Google Scholar

24 

Cerdá B, Periago P, Espín JC and Tomás-Barberán FA: Identification of urolithin a as a metabolite produced by human colon microflora from ellagic acid and related compounds. J Agric Food Chem. 53:5571–5576. 2005.PubMed/NCBI View Article : Google Scholar

25 

Lucas R, Alcantara D and Morales JC: A concise synthesis of glucuronide metabolites of urolithin-B, resveratrol, and hydroxytyrosol. Carbohydr Res. 344:1340–1346. 2009.PubMed/NCBI View Article : Google Scholar

26 

Rupiani S, Guidotti L, Manerba M, Di Ianni L, Giacomini E, Falchi F, Di Stefano G, Roberti M and Recanatini M: Synthesis of natural urolithin M6, a galloflavin mimetic, as a potential inhibitor of lactate dehydrogenase A. Org Biomol Chem. 14:10981–10987. 2016.PubMed/NCBI View Article : Google Scholar

27 

Nuñez-Sánchez MA, García-Villalba R, Monedero-Saiz T, García-Talavera NV, Gómez-Sánchez MB, Sánchez-Álvarez C, García-Albert AM, Rodríguez-Gil FJ, Ruiz-Marín M, Pastor-Quirante FA, et al: Targeted metabolic profiling of pomegranate polyphenols and urolithins in plasma, urine and colon tissues from colorectal cancer patients. Mol Nutr Food Res. 58:1199–1211. 2014.PubMed/NCBI View Article : Google Scholar

28 

González-Barrio R, Truchado P, Ito H, Espin JC and Tomás-Barberán FA: UV and MS identification of Urolithins and Nasutins, the bioavailable metabolites of ellagitannins and ellagic acid in different mammals. J Agric Food Chem. 59:1152–1162. 2011.PubMed/NCBI View Article : Google Scholar

29 

Seeram NP, Henning SM, Zhang Y, Suchard M, Li Z and Heber D: Pomegranate juice ellagitannin metabolites are present in human plasma and some persist in urine for up to 48 hours. J Nutr. 136:2481–2485. 2006.PubMed/NCBI View Article : Google Scholar

30 

García-Villalba R, Espín JC and Tomás-Barberán FA: Chromatographic and spectroscopic characterization of urolithins for their determination in biological samples after the intake of foods containing ellagitannins and ellagic acid. J Chromatogr A. 1428:162–175. 2016.PubMed/NCBI View Article : Google Scholar

31 

Giorgio C, Mena P, Del Rio D, Brighenti F, Barocelli E, Hassan-Mohamed I, Callegari D, Lodola A and Tognolini M: The ellagitannin colonic metabolite urolithin D selectively inhibits EphA2 phosphorylation in prostate cancer cells. Mol Nutr Food Res. 59:2155–2167. 2015.PubMed/NCBI View Article : Google Scholar

32 

Fan M, Qin K, Ding F, Huang Y, Wang X and Cai B: Identification and differentiation of major components in three different ‘Sheng-ma’ crude drug species by UPLC/Q-TOF-MS. Acta Pharm Sin B. 7:185–192. 2017.PubMed/NCBI View Article : Google Scholar

33 

Vattem DA and Shetty K: Biological functionality of ellagic acid: A review. J Food Biochem. 29:234–266. 2005.

34 

Jadhav PD and Laddha KS: Synthesis of new ellagic acid derivatives. Indian J Chem B. 45:1551–1553. 2006.

35 

Huang ZH, Xu Y, Wang Q and Gao XY: Metabolism and mutual biotransformations of anthraquinones and anthrones in rhubarb by human intestinal flora using UPLC-Q-TOF/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 1104:59–66. 2019.PubMed/NCBI View Article : Google Scholar

36 

Piwowarski JP, Granica S, Zwierzyńska M, Stefańska J, Schopohl P, Melzig MF and Kiss AK: Role of human gut microbiota metabolism in the anti-inflammatory effect of traditionally used ellagitannin-rich plant materials. J Ethnopharmacol. 155:801–809. 2014.PubMed/NCBI View Article : Google Scholar

37 

Stolarczyk M, Piwowarski JP, Granica S, Stefanska J, Naruszewicz M and Kiss AK: Extracts from Epilobium sp. herbs, their components and gut microbiota metabolites of Epilobium ellagitannins, urolithins, inhibit hormone-dependent prostate cancer cells-(LNCaP) proliferation and PSA secretion. Phytother Res. 27:1842–1848. 2013.PubMed/NCBI View Article : Google Scholar

38 

Haddad EH, Gaban-Chong N, Oda K and Sabaté J: Effect of a walnut meal on postprandial oxidative stress and antioxidants in healthy individuals. Nutr J. 13(4)2014.PubMed/NCBI View Article : Google Scholar

39 

Han QA, Yan C, Wang L, Li G, Xu Y and Xia X: Urolithin A attenuates ox-LDL-induced endothelial dysfunction partly by modulating microRNA-27 and ERK/PPAR-γ pathway. Mol Nutr Food Res. 60:1933–1943. 2016.PubMed/NCBI View Article : Google Scholar

40 

Ryu D, Mouchiroud L, Andreux PA, Katsyuba E, Moullan N, Nicolet-Dit-Félix AA, Williams EG, Jha P, Lo Sasso G, Huzard D, et al: Urolithin A induces mitophagy and prolongs lifespan in C. elegans and increases muscle function in rodents. Nat Med. 22:879–893. 2016.PubMed/NCBI View Article : Google Scholar

41 

Chen P, Chen F, Lei J, Li Q and Zhou B: Activation of the miR-34a-mediated SIRT1/mTOR signaling pathway by urolithin A attenuates D-galactose-induced brain aging in mice. Neurotherapeutics. 16:1269–1282. 2019.PubMed/NCBI View Article : Google Scholar

42 

Kumar RS, Narasingappa RB, Joshi CG, Girish TK, Prasada Rao UJ and Danagoudar A: Evaluation of Cassia tora Linn. against oxidative stress-induced DNA and cell membrane damage. J Pharm Bioallied Sci. 9:33–43. 2017.PubMed/NCBI View Article : Google Scholar

43 

David LA, Maurice CF, Carmody RN, Gootenberg DB, Button JE, Wolfe BE, Ling AV, Devlin AS, Varma Y, Fischbach MA, et al: Diet rapidly and reproducibly alters the human gut microbiome. Nature. 505:559–563. 2014.PubMed/NCBI View Article : Google Scholar

44 

Ezenwa VO, Gerardo NM, Inouye DW, Medina M and Xavier JB: Microbiology. Animal behavior and the microbiome. Science. 338:198–199. 2012.PubMed/NCBI View Article : Google Scholar

45 

Espín JC, Larrosa M, García-Conesa MT and Tomás-Barberán F: Biological significance of urolithins, the gut microbial ellagic Acid-derived metabolites: The evidence so far. Evid Based Complement Alternat Med. 2013(270418)2013.PubMed/NCBI View Article : Google Scholar

46 

Selma MV, Beltrán D, García-Villalba R, Espín JC and Tomás-Barberán FA: Description of urolithin production capacity from ellagic acid of two human intestinal Gordonibacter species. Food Funct. 5:1779–1784. 2014.PubMed/NCBI View Article : Google Scholar

47 

Clarke G, Sandhu KV, Griffin BT, Dinan TG, Cryan JF and Hyland NP: Gut reactions: Breaking down xenobiotic-microbiome interactions. Pharmacol Rev. 71:198–224. 2019.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wu SH, Li HB, Li GL, Lv N and Qi YJ: Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS. Exp Ther Med 19: 3305-3315, 2020.
APA
Wu, S., Li, H., Li, G., Lv, N., & Qi, Y. (2020). Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS. Experimental and Therapeutic Medicine, 19, 3305-3315. https://doi.org/10.3892/etm.2020.8585
MLA
Wu, S., Li, H., Li, G., Lv, N., Qi, Y."Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS". Experimental and Therapeutic Medicine 19.5 (2020): 3305-3315.
Chicago
Wu, S., Li, H., Li, G., Lv, N., Qi, Y."Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS". Experimental and Therapeutic Medicine 19, no. 5 (2020): 3305-3315. https://doi.org/10.3892/etm.2020.8585
Copy and paste a formatted citation
x
Spandidos Publications style
Wu SH, Li HB, Li GL, Lv N and Qi YJ: Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS. Exp Ther Med 19: 3305-3315, 2020.
APA
Wu, S., Li, H., Li, G., Lv, N., & Qi, Y. (2020). Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS. Experimental and Therapeutic Medicine, 19, 3305-3315. https://doi.org/10.3892/etm.2020.8585
MLA
Wu, S., Li, H., Li, G., Lv, N., Qi, Y."Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS". Experimental and Therapeutic Medicine 19.5 (2020): 3305-3315.
Chicago
Wu, S., Li, H., Li, G., Lv, N., Qi, Y."Metabolite identification of gut microflora‑cassia seed interactions using UPLC‑QTOF/MS". Experimental and Therapeutic Medicine 19, no. 5 (2020): 3305-3315. https://doi.org/10.3892/etm.2020.8585
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