Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
June-2020 Volume 19 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2020 Volume 19 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats

  • Authors:
    • Sunmin Park
    • Ting Zhang
    • Xuangao Wu
    • Jing Yi Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Food and Nutrition, Obesity/Diabetes Center, Hoseo University, Asan, Chungcheong 336‑795, Republic of Korea
    Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3611-3619
    |
    Published online on: April 2, 2020
       https://doi.org/10.3892/etm.2020.8636
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The liver is an important organ for the removal of toxins and utilization of nutrients. The present study then investigated whether a mixture of mulberry water extracts and silk amino acids protected against acute liver damage in rats induced by intraperitoneal injection of D‑galactosamine and the action mechanism. D‑galactosamine injection is widely used to develop experimental animal models of acute hepatic disease. In the present study, male Sprague‑Dawley rats received intraperitoneal injection of D‑galactosamine followed by 200 and 600 mg/kg body weight (BW) of mulberry extracts and silk amino acids (1:3, w/w; MS1:3‑L and MS1:3‑H), the same amounts of MS with different ratios (1:5, w/w; MS1:5‑L and MS1:5‑H), and 600 mg/kg bw cellulose (control) for 1 week. The normal‑control group received an injection of saline instead of D‑galactosamine with the same diet as the control group. D‑galactosamine injection (control rats) increased serum ALT, AST and γ‑GPT levels, indicating the induction of acute liver damage. The control rats also exhibited reduced glycogen depositions, which contributed to increasing fat synthesis from glucose and elevated serum triglyceride levels. Oxidative stress and inflammation in the liver of the control increased in response to the decreasing antioxidant activity and mRNA expression and increasing TNF‑α expression, respectively. Both MS1:3 and MS1:5 reduced serum ALT, AST and γ‑GPT levels to ameliorate liver damage. MS1:3 reduced oxidative stress by increasing the activity and expression of antioxidant enzymes, whereas MS1:5 decreased the expression TNF‑α in the liver. MS1:3 and MS1:5 improved the necrosis of hepatocytes in H&E staining, which was associated with increased glycogen deposition in PAS staining. MS1:5 had better effects on glycogen accumulation. In conclusion, MS1:3 and MS1:5 can be used as therapeutic agents for acute liver damage.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Lim DW, Kim H, Park JY, Kim JE, Moon JY, Park SD and Park WH: Amomum cardamomum L. ethyl acetate fraction protects against carbon tetrachloride-induced liver injury via an antioxidant mechanism in rats. BMC Complement Altern Med. 16(155)2016.PubMed/NCBI View Article : Google Scholar

2 

Hsu LS, Ho HH, Lin MC, Chyau CC, Peng JS and Wang CJ: Mulberry water extracts (MWEs) ameliorated carbon tetrachloride-induced liver damages in rat. Food Chem Toxicol. 50:3086–3093. 2012.PubMed/NCBI View Article : Google Scholar

3 

Radhiga T, Sundaresan A, Viswanathan P and Pugalendi KV: Effect of protocatechuic acid on lipid profile and DNA damage in D-galactosamine-induced hepatotoxic rats. J Basic Clin Physiol Pharmacol. 27:505–514. 2016.PubMed/NCBI View Article : Google Scholar

4 

Park S, Kim DS, Wu X and Q JY: Mulberry and dandelion water extracts prevent alcohol-induced steatosis with alleviating gut microbiome dysbiosis. Exp Biol Med (Maywood). 243:882–894. 2018.PubMed/NCBI View Article : Google Scholar

5 

Decker K and Keppler D: Galactosamine hepatitis: Key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death. Rev Physiol Biochem Pharmacol. 77–106. 1974.PubMed/NCBI View Article : Google Scholar

6 

Ou TT, Kuo CY, Chyau CC, Lee HJ, Peng JS and Wang CJ: Improvement of lipopolysaccharide-induced hepatic injuries and inflammation with mulberry extracts. J Sci Food Agric. 93:1880–1886. 2013.PubMed/NCBI View Article : Google Scholar

7 

Yan F, Chen Y, Azat R and Zheng X: Mulberry anthocyanin extract ameliorates oxidative damage in HepG2 cells and prolongs the lifespan of Caenorhabditis elegans through MAPK and Nrf2 pathways. Oxid Med Cell Longev. 2017(7956158)2017.PubMed/NCBI View Article : Google Scholar

8 

Yang HJ, Kim MJ, Kang ES, Kim DS and Park S: Red mulberry fruit aqueous extract and silk proteins accelerate acute ethanol metabolism and promote the antioxidant enzyme systems in rats. Mol Med Rep. 18:1197–1205. 2018.PubMed/NCBI View Article : Google Scholar

9 

Lopez-Terrada D, Cheung SW, Finegold MJ and Knowles BB: Hep G2 is a hepatoblastoma-derived cell line. Hum Pathol. 40:1512–1515. 2009.PubMed/NCBI View Article : Google Scholar

10 

Jeong SY, Kang S, Hua CS, Ting Z and Park S: Synbiotic effects of beta-glucans from cauliflower mushroom and Lactobacillus fermentum on metabolic changes and gut microbiome in estrogen-deficient rats. Genes Nutr. 12(31)2017.PubMed/NCBI View Article : Google Scholar

11 

Moon NR, Kang S and Park S: Consumption of ellagic acid and dihydromyricetin synergistically protects against UV-B induced photoaging, possibly by activating both TGF-β1 and wnt signaling pathways. J Photochem Photobiol B. 178:92–100. 2018.PubMed/NCBI View Article : Google Scholar

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

13 

Lim HH, Lee SO, Kim SY, Yang SJ and Lim Y: Anti-inflammatory and antiobesity effects of mulberry leaf and fruit extract on high fat diet-induced obesity. Exp Biol Med (Maywood). 238:1160–1169. 2013.PubMed/NCBI View Article : Google Scholar

14 

Ganai AA, Khan AA, Malik ZA and Farooqi H: Genistein modulates the expression of NF-κB and MAPK (P-38 and ERK1/2), thereby attenuating d-Galactosamine induced fulminant hepatic failure in Wistar rats. Toxicol Appl Pharmacol. 283:139–146. 2015.PubMed/NCBI View Article : Google Scholar

15 

Sakaguchi S and Yokota K: Role of Ca2+ on endotoxin-sensitivity by galactosamine challenge: Lipid peroxide formation and hepatotoxicity in zymosan-primed mice. Pharmacol Toxicol. 77:81–86. 1995.PubMed/NCBI View Article : Google Scholar

16 

Li YG, Ji DF, Chen S and Hu GY: Protective effects of sericin protein on alcohol-mediated liver damage in mice. Alcohol Alcohol. 43:246–253. 2008.PubMed/NCBI View Article : Google Scholar

17 

Tang CC, Huang HP, Lee YJ, Tang YH and Wang CJ: Hepatoprotective effect of mulberry water extracts on ethanol-induced liver injury via anti-inflammation and inhibition of lipogenesis in C57BL/6J mice. Food Chem Toxicol. 62:786–796. 2013.PubMed/NCBI View Article : Google Scholar

18 

Saracyn M, Zdanowski R, Brytan M, Kade G, Nowak Z, Patera J, Dyrla P, Gil J and Wankowicz Z: D-galactosamine intoxication in experimental animals: Is it only an experimental model of acute liver failure? Med Sci Monit. 21:1469–1477. 2015.PubMed/NCBI View Article : Google Scholar

19 

Ganai AA and Husain M: Genistein attenuates D-GalN induced liver fibrosis/chronic liver damage in rats by blocking the TGF-β/Smad signaling pathways. Chem Biol Interact. 261:80–85. 2017.PubMed/NCBI View Article : Google Scholar

20 

Banu S, Bhaskar B and Balasekar P: Hepatoprotective and antioxidant activity of Leucas aspera against D-galactosamine induced liver damage in rats. Pharm Biol. 50:1592–1595. 2012.PubMed/NCBI View Article : Google Scholar

21 

Do SG, Park JH, Nam H, Kim JB, Lee JY, Oh YS and Suh JG: Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic β cell mass in C57BL/KsJ-db/db mice. J Vet Sci. 13:339–344. 2012.PubMed/NCBI View Article : Google Scholar

22 

Song Z, Zhang M, Xue R, Cao G and Gong C: Reducing blood glucose levels in TIDM mice with an orally administered extract of sericin from hIGF-I-transgenic silkworm cocoons. Food Chem Toxicol. 67:249–254. 2014.PubMed/NCBI View Article : Google Scholar

23 

Farah BL, Sinha RA, Wu Y, Singh BK, Lim A, Hirayama M, Landau DJ, Bay BH, Koeberl DD and Yen PM: Hepatic mitochondrial dysfunction is a feature of Glycogen Storage Disease Type Ia (GSDIa). Sci Rep. 7(44408)2017.PubMed/NCBI View Article : Google Scholar

24 

Henrique da Silva G, Barros PP, Silva Goncalves GM and Landi MA: Hepatoprotective effect of Lycopodium clavatum 30CH on experimental model of paracetamol-induced liver damage in rats. Homeopathy. 104:29–35. 2015.PubMed/NCBI View Article : Google Scholar

25 

Irimia JM, Meyer CM, Segvich DM, Surendran S, DePaoli-Roach AA, Morral N and Roach PJ: Lack of liver glycogen causes hepatic insulin resistance and steatosis in mice. J Biol Chem. 292:10455–10464. 2017.PubMed/NCBI View Article : Google Scholar

26 

Crescenzo R, Bianco F, Mazzoli A, Giacco A, Liverini G and Iossa S: A possible link between hepatic mitochondrial dysfunction and diet-induced insulin resistance. Eur J Nutr. 55:1–6. 2016.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Park S, Zhang T, Wu X and Qiu J: A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats. Exp Ther Med 19: 3611-3619, 2020.
APA
Park, S., Zhang, T., Wu, X., & Qiu, J. (2020). A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats. Experimental and Therapeutic Medicine, 19, 3611-3619. https://doi.org/10.3892/etm.2020.8636
MLA
Park, S., Zhang, T., Wu, X., Qiu, J."A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats". Experimental and Therapeutic Medicine 19.6 (2020): 3611-3619.
Chicago
Park, S., Zhang, T., Wu, X., Qiu, J."A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats". Experimental and Therapeutic Medicine 19, no. 6 (2020): 3611-3619. https://doi.org/10.3892/etm.2020.8636
Copy and paste a formatted citation
x
Spandidos Publications style
Park S, Zhang T, Wu X and Qiu J: A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats. Exp Ther Med 19: 3611-3619, 2020.
APA
Park, S., Zhang, T., Wu, X., & Qiu, J. (2020). A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats. Experimental and Therapeutic Medicine, 19, 3611-3619. https://doi.org/10.3892/etm.2020.8636
MLA
Park, S., Zhang, T., Wu, X., Qiu, J."A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats". Experimental and Therapeutic Medicine 19.6 (2020): 3611-3619.
Chicago
Park, S., Zhang, T., Wu, X., Qiu, J."A mixture of mulberry and silk amino acids protected against D‑galactosamine induced acute liver damage by attenuating oxidative stress and inflammation in HepG2 cells and rats". Experimental and Therapeutic Medicine 19, no. 6 (2020): 3611-3619. https://doi.org/10.3892/etm.2020.8636
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team