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Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro

  • Authors:
    • Ziqiang Xu
    • Jihao Chen
    • Jian Cai
    • Yunbei Xiao
    • Qinquan Wang
    • Shuowei Chen
    • Lei Zhang
    • Zhigang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Transplantation, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Andrology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, The Second Department of Medicine, Renji School, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Urology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3497-3504
    |
    Published online on: April 2, 2020
       https://doi.org/10.3892/etm.2020.8637
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Abstract

Adenosine triphosphate (ATP) levels are closely associated with diabetes‑related erectile dysfunction (DMED). Mitochondrial ATP synthase serves a key role in ATP production. The present study aimed to investigate the relationship between F1‑ATP synthase and DMED in vivo and in vitro. The F1‑ATP synthase expression levels in corpus cavernosum tissues from rats with DMED were examined. F1‑ATP synthase expression was found to be lower in corpus cavernosum tissues from rats with DMED compared with healthy controls, suggesting a role for ATP synthase under high glucose conditions. In addition, the present study also demonstrated that hyperglycemia could downregulate F1‑ATP synthase expression in rat corpus cavernosum smooth muscle cells (CCSMCs) in vitro. The overexpression of F1‑ATP synthase in CCSMCs influenced the phenotypic CCSMC transformation, upregulated eNOS expression, increased cGMP levels and reduced CCSMC apoptosis under high glucose in vitro. In conclusion, the present study indicates that the upregulation of mitochondrial ATP synthase expression may improve CCSMC function, suggesting that mitochondrial ATP synthase could serve as a potential therapeutic target for the treatment of DMED.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Z, Chen J, Cai J, Xiao Y, Wang Q, Chen S, Zhang L and Wu Z: Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro. Exp Ther Med 19: 3497-3504, 2020.
APA
Xu, Z., Chen, J., Cai, J., Xiao, Y., Wang, Q., Chen, S. ... Wu, Z. (2020). Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro. Experimental and Therapeutic Medicine, 19, 3497-3504. https://doi.org/10.3892/etm.2020.8637
MLA
Xu, Z., Chen, J., Cai, J., Xiao, Y., Wang, Q., Chen, S., Zhang, L., Wu, Z."Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro". Experimental and Therapeutic Medicine 19.6 (2020): 3497-3504.
Chicago
Xu, Z., Chen, J., Cai, J., Xiao, Y., Wang, Q., Chen, S., Zhang, L., Wu, Z."Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro". Experimental and Therapeutic Medicine 19, no. 6 (2020): 3497-3504. https://doi.org/10.3892/etm.2020.8637
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Z, Chen J, Cai J, Xiao Y, Wang Q, Chen S, Zhang L and Wu Z: Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro. Exp Ther Med 19: 3497-3504, 2020.
APA
Xu, Z., Chen, J., Cai, J., Xiao, Y., Wang, Q., Chen, S. ... Wu, Z. (2020). Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro. Experimental and Therapeutic Medicine, 19, 3497-3504. https://doi.org/10.3892/etm.2020.8637
MLA
Xu, Z., Chen, J., Cai, J., Xiao, Y., Wang, Q., Chen, S., Zhang, L., Wu, Z."Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro". Experimental and Therapeutic Medicine 19.6 (2020): 3497-3504.
Chicago
Xu, Z., Chen, J., Cai, J., Xiao, Y., Wang, Q., Chen, S., Zhang, L., Wu, Z."Mitochondrial ATP synthase regulates corpus cavernosum smooth muscle cell function in vivo and in vitro". Experimental and Therapeutic Medicine 19, no. 6 (2020): 3497-3504. https://doi.org/10.3892/etm.2020.8637
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