Open Access

Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis

  • Authors:
    • Juan Kang
    • Dongyun Feng
    • Feng Yang
    • Xiaojia Tian
    • Wenjuan Han
    • Hongge Jia
  • View Affiliations

  • Published online on: May 5, 2020     https://doi.org/10.3892/etm.2020.8716
  • Pages: 219-226
  • Copyright: © Kang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Idiopathic inflammatory myopathies (IIMs) are a group of autoimmune inflammatory muscle diseases. Rapamycin has been shown to ameliorate inflammation and improve muscle function in a mouse model of experimental autoimmune myositis (EAM). In the present study, the therapeutic effect of rapamycin was compared with methylprednisolone (MP) on EAM. Mice were injected with myosin for 10 days to induce EAM and were subsequently treated with rapamycin (1.5 mg/kg), MP (40 mg/kg) or placebo (DMSO) for 14 days. The rapamycin‑treated group exhibited significantly decreased severe inflammation and improved muscle strength compared with the MP‑treated group. The plasma transforming growth factor‑β (TGF‑β) concentration in the rapamycin‑treated group was significantly higher compared with the placebo group. However, both treatment groups exhibited significantly lower plasma interleukin‑10 levels compared with the placebo group. Moreover, splenic regulatory T cell frequency in both the rapamycin‑ and MP‑treated animals was significantly lower than that in the animals of the placebo group. Rapamycin showed better immune suppressive effects than MP in this model of EAM, and these effects were likely to be mediated by the TGF‑β signaling pathway.
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July-2020
Volume 20 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kang J, Feng D, Yang F, Tian X, Han W and Jia H: Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis. Exp Ther Med 20: 219-226, 2020
APA
Kang, J., Feng, D., Yang, F., Tian, X., Han, W., & Jia, H. (2020). Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis. Experimental and Therapeutic Medicine, 20, 219-226. https://doi.org/10.3892/etm.2020.8716
MLA
Kang, J., Feng, D., Yang, F., Tian, X., Han, W., Jia, H."Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis". Experimental and Therapeutic Medicine 20.1 (2020): 219-226.
Chicago
Kang, J., Feng, D., Yang, F., Tian, X., Han, W., Jia, H."Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis". Experimental and Therapeutic Medicine 20, no. 1 (2020): 219-226. https://doi.org/10.3892/etm.2020.8716