Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2020 Volume 20 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2020 Volume 20 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway

  • Authors:
    • Hailing Wen
    • Liang Xing
    • Kui Sun
    • Changshuan Xiao
    • Xiangxi Meng
    • Jingzhe Yang
  • View Affiliations / Copyright

    Affiliations: Department of Burns and Plastic Surgery, Affiliated Hospital of Chengde Medical University, Chengde, Hebei 067000, P.R. China, Department of Burns and Plastic Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
  • Pages: 591-598
    |
    Published online on: May 7, 2020
       https://doi.org/10.3892/etm.2020.8725
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Severe burns may lead to intestinal inflammation and oxidative stress, resulting in intestinal barrier damage and gut dysfunction. Loganin, an iridoid glycoside compound, has been isolated from Cornus officinalis Sieb. et Zucc; however, its role in the treatment of burn injury is yet to be fully elucidated. Therefore, the present study examined the effect of loganin administration on burn‑induced intestinal inflammation and oxidative stress after severe burns in male Sprague‑Dawley rats. Histological injury was assessed by hematoxylin and eosin staining. Furthermore, cytokine expression in intestinal tissues was measured by ELISA and reverse transcription‑quantitative PCR. Antioxidative activities were assessed by determining the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH‑Px) and malondialdehyde (MDA). Apoptosis was detected by flow cytometry. Apoptosis‑related proteins, toll‑like receptor 4 (TLR4) protein and NF‑κB translocation were examined by western blotting. Immunohistochemical staining was used to observe TLR4 and NF‑κB p65 expression in intestinal tissues. The present study suggested that loganin administration significantly reduced burn injury‑induced intestinal histological changes, tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑1β production and oxidative stress, evidenced by decreased ROS levels and MDA content (P<0.05). Furthermore, loganin increased SOD, CAT and GSH‑Px levels and intestinal epithelial cell apoptosis. Loganin treatment also significantly inhibited activation of the TLR4/NF‑κB signaling pathway in the intestine of severely burned rats (P<0.05). In conclusion, loganin reduced burns‑induced intestinal inflammation and oxidative stress, potentially by regulating the TLR4/NF‑κB signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Fay KT, Ford ML and Coopersmith CM: The intestinal microenvironment in sepsis. Biochim Biophys Acta Mol Basis Dis. 1863:2574–2583. 2017.PubMed/NCBI View Article : Google Scholar

2 

He W, Wang Y, Wang P and Wang F: Intestinal barrier dysfunction in severe burn injury. Burns Trauma. 7(24)2019.PubMed/NCBI View Article : Google Scholar

3 

Qin Y, Hamilton JL, Bird MD, Chen MM, Ramirez L, Zahs A, Kovacs EJ and Makowski L: Adipose inflammation and macrophage infiltration after binge ethanol and burn injury. Alcohol Clin Exp Res. 38:204–213. 2013.PubMed/NCBI View Article : Google Scholar

4 

Earley ZM, Akhtar S, Green SJ, Naqib A, Khan O, Cannon AR, Hammer AM, Morris NL, Li X, Eberhardt JM, et al: Burn injury alters the intestinal microbiome and increases gut permeability and bacterial translocation. PLoS One. 10(e0129996)2015.PubMed/NCBI View Article : Google Scholar

5 

Gong ZY, Yuan ZQ, Dong ZW and Peng YZ: Glutamine with probiotics attenuates intestinal inflammation and oxidative stress in a rat burn injury model through altered iNOS gene aberrant methylation. Am J Transl Res. 9:2535–2547. 2017.PubMed/NCBI

6 

Haines RJ, Wang CY, Yang CGY, Eitnier RA, Wang F and Wu MH: Targeting palmitoyl acyltransferase ZDHHC21 improves gut epithelial barrier dysfunction resulting from burn-induced systemic inflammation. Am J Physiol Gastrointest Liver Physiol. 313:G549–G557. 2017.PubMed/NCBI View Article : Google Scholar

7 

Li JY, Sheng ZY, Lu Y, Yu Y and Zhou BT: Severe trauma induced intestinal barrier function injury and protection. World Chin J Digestol. 8:1093–1096. 2000.

8 

Wang Z, Chen R, Zhu Z, Zhang X and Wang S: Effects of insulin combined with ethyl pyruvate on inflammatory response and oxidative stress in multiple-organ dysfunction syndrome rats with severe burns. Am J Emerg Med. 34:2154–2158. 2016.PubMed/NCBI View Article : Google Scholar

9 

Wang L, Liu XH, Chen H, Chen ZY, Weng XD, Qiu T and Liu L: Picroside II protects rat kidney against ischemia/reperfusion-induced oxidative stress and inflammation by the TLR4/NF-κB pathway. Exp Ther Med. 9:1253–1258. 2015.PubMed/NCBI View Article : Google Scholar

10 

Zhang L, Sun S, Li W, Zhang W, Wang X and Yang SY: Effect of Scutellarin inhibits collagen-induced arthritis through TLR4/NF-κB-mediated inflammation. Mol Med Rep. 16:5555–5560. 2017.PubMed/NCBI View Article : Google Scholar

11 

Latorre E, Mendoza C, Layunta E, Alcalde AI and Mesonero JE: TLR2, TLR3, and TLR4 activation specifically alters the oxidative status of intestinal epithelial cells. Cell Stress Chaperones. 19:289–293. 2014.PubMed/NCBI View Article : Google Scholar

12 

Zhou M, Xu W, Wang J, Yan J, Shi Y, Zhang C, Ge W, Wu J, Du P and Chen Y: Boosting mTOR-dependent autophagy via upstream TLR4-MyD88-MAPK signalling and downstream NF-κB pathway quenches intestinal inflammation and oxidative stress injury. EBioMedicine. 35:345–360. 2018.PubMed/NCBI View Article : Google Scholar

13 

Xu H, Shen J, Liu H, Shi Y, Li L and Wei M: Morroniside and loganin extracted from Cornus officinalis have protective effects on rat mesangial cell proliferation exposed to advanced glycation end products by preventing oxidative stress. Can J Physiol Pharmacol. 84:1267–1273. 2006.PubMed/NCBI View Article : Google Scholar

14 

Kim MJ, Bae GS, Jo IJ, Choi SB, Kim DG, Shin JY, Lee SK, Kim MJ, Shin S, Song HJ and Park SJ: Loganin protects against pancreatitis by inhibiting NF-κB activation. Eur J Pharmacol. 765:541–550. 2015.PubMed/NCBI View Article : Google Scholar

15 

Tseng YT, Chen CS, Jong YJ, Chang FR and Lo YC: Loganin possesses neuroprotective properties, restores SMN protein and activates protein synthesis positive regulator Akt/mTOR in experimental models of spinal muscular atrophy. Pharmacol Res. 111:58–75. 2016.PubMed/NCBI View Article : Google Scholar

16 

Li Y, Li Z, Shi L, Zhao C, Shen B, Tian Y and Feng H: Loganin inhibits the inflammatory response in mouse 3T3L1 adipocytes and mouse model. Int Immunopharmacol. 36:173–179. 2016.PubMed/NCBI View Article : Google Scholar

17 

Rajabi M, Mohaddes G, Farajdokht F, Nayebi Rad S, Mesgari M and Babri S: Impact of loganin on pro-inflammatory cytokines and depression- and anxiety-like behaviors in male diabetic rats. Physiol Int. 105:199–209. 2018.PubMed/NCBI View Article : Google Scholar

18 

Zhou Q, Price DD, Caudle RM and Verne GN: Visceral and somatic hypersensitivity in a subset of rats following TNBS-induced colitis. Pain. 134:9–15. 2008.PubMed/NCBI View Article : Google Scholar

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

20 

Nakazawa H, Chang K, Shinozaki S, Yasukawa T, Ishimaru K, Yasuhara S, Yu YM, Martyn JA, Tompkins RG, Shimokado K and Kaneki M: iNOS as a driver of inflammation and apoptosis in mouse skeletal muscle after burn injury: Possible involvement of Sirt1 S-Nitrosylation-mediated acetylation of p65 NF-κB and p53. PLoS One. 12(e0170391)2017.PubMed/NCBI View Article : Google Scholar

21 

Möller H, Falster K, Ivers R, Clapham K, Harvey L and Jorm L: High rates of hospitalised burn injury in Indigenous children living in remote areas: A population data linkage study. Aust N Z J Public Health. 42:108–109. 2018.PubMed/NCBI View Article : Google Scholar

22 

Botchey IM Jr, Hung YW, Bachani AM, Paruk F, Mehmood A, Saidi H and Hyder AA: Epidemiology and outcomes of injuries in Kenya: A multisite surveillance study. Surgery. 162 (Suppl):S45–S53. 2017.PubMed/NCBI View Article : Google Scholar

23 

Yuan ZQ, Peng YZ, Li XL, Huang YS and Yang ZC: Induction of heat shock protein 70 by sodium arsenite attenuates burn-induced intestinal injury in severe burned rats. Burns. 34:247–253. 2008.PubMed/NCBI View Article : Google Scholar

24 

Sun YX, Han LN, Gao Z, Wu XS, Zhou M, Wang F, Peszel A and Chen XL: 200 mM hypertonic saline resuscitation attenuates intestinal injury and inhibits p38 signaling in rats after severe burn trauma. Burns. 43:1693–1701. 2017.PubMed/NCBI View Article : Google Scholar

25 

O'Dea KP, Porter JR, Tirlapur N, Katbeh U, Singh S, Handy JM and Takata M: Circulating microvesicles are elevated acutely following major burns injury and associated with clinical severity. PLoS One. 11(e0167801)2016.PubMed/NCBI View Article : Google Scholar

26 

Minden-Birkenmaier BA, Meadows MB, Cherukuri K, Smeltzer MP, Smith RA, Radic MZ and Bowlin GL: The effect of manuka honey on dHL-60 cytokine, chemokine, and matrix-degrading enzyme release under inflammatory conditions. Med One. 4(e190005)2019.PubMed/NCBI View Article : Google Scholar

27 

Fei Y, Sun L, Yuan C, Jiang M, Lou Q and Xu Y: CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells. Int J Mol Med. 41:3501–3508. 2018.PubMed/NCBI View Article : Google Scholar

28 

Carter SR, Zahs A, Palmer JL, Wang L, Ramirez L, Gamelli RL and Kovacs EJ: Intestinal barrier disruption as a cause of mortality in combined radiation and burn injury. Shock. 40:281–289. 2013.PubMed/NCBI View Article : Google Scholar

29 

Li YM, Wang HB, Zheng JG, Bai XD, Zhao ZK, Li JY and Hu S: Dimethyl sulfoxide inhibits zymosan-induced intestinal inflammation and barrier dysfunction. World J Gastroenterol. 21:10853–10865. 2015.PubMed/NCBI View Article : Google Scholar

30 

Kwon SH, Kim JA, Hong SI, Jung YH, Kim HC, Lee SY and Jang CG: Loganin protects against hydrogen peroxide-induced apoptosis by inhibiting phosphorylation of JNK, p38, and ERK 1/2 MAPKs in SH-SY5Y cells. Neurochem Int. 58:533–541. 2011.PubMed/NCBI View Article : Google Scholar

31 

Park CH, Tanaka T, Kim JH, Cho EJ, Park JC, Shibahara N and Yokozawa T: Hepato-protective effects of loganin, iridoid glycoside from Corni Fructus, against hyperglycemia-activated signaling pathway in liver of type 2 diabetic db/db mice. Toxicology. 290:14–21. 2011.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wen H, Xing L, Sun K, Xiao C, Meng X and Yang J: Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway. Exp Ther Med 20: 591-598, 2020.
APA
Wen, H., Xing, L., Sun, K., Xiao, C., Meng, X., & Yang, J. (2020). Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 20, 591-598. https://doi.org/10.3892/etm.2020.8725
MLA
Wen, H., Xing, L., Sun, K., Xiao, C., Meng, X., Yang, J."Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 20.1 (2020): 591-598.
Chicago
Wen, H., Xing, L., Sun, K., Xiao, C., Meng, X., Yang, J."Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 20, no. 1 (2020): 591-598. https://doi.org/10.3892/etm.2020.8725
Copy and paste a formatted citation
x
Spandidos Publications style
Wen H, Xing L, Sun K, Xiao C, Meng X and Yang J: Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway. Exp Ther Med 20: 591-598, 2020.
APA
Wen, H., Xing, L., Sun, K., Xiao, C., Meng, X., & Yang, J. (2020). Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 20, 591-598. https://doi.org/10.3892/etm.2020.8725
MLA
Wen, H., Xing, L., Sun, K., Xiao, C., Meng, X., Yang, J."Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 20.1 (2020): 591-598.
Chicago
Wen, H., Xing, L., Sun, K., Xiao, C., Meng, X., Yang, J."Loganin attenuates intestinal injury in severely burned rats by regulating the toll‑like receptor 4/NF‑κB signaling pathway". Experimental and Therapeutic Medicine 20, no. 1 (2020): 591-598. https://doi.org/10.3892/etm.2020.8725
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team