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Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine

  • Authors:
    • Juan Yang
    • Guowei Wang
    • Haining Li
    • Wenli Zheng
    • Burui Guo
    • Zhenhai Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 926-932
    |
    Published online on: May 13, 2020
       https://doi.org/10.3892/etm.2020.8745
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Abstract

The ability to inhibit host macrophage apoptosis is one of the survival strategies of intracellular bacteria, including Brucella. In the present study the role of Mg2+/Mn2+ dependent protein phosphatase 1A (PPM1A) in the apoptosis of Brucella suis (B. suis) strain 2 vaccine‑infected BV2 cells was investigated. Compared with control cells, the protein expression levels of cleaved caspase‑3 were markedly increased in PPM1A short hairpin (sh)RNA‑transfected BV2 cells. Flow cytometry analysis showed that treatment with JNK activator anisomycin significantly increased the rate of apoptosis in BV2 cells in comparison with the control cells. Furthermore, PPM1A shRNA significantly increased the levels of JNK phosphorylation and the levels of cleaved caspase‑3 in BV2 cells infected with B. suis strain 2 in comparison with the control cells. DAPI staining showed nuclear condensation in B. suis infected BV2 cells transfected with PPM1A shRNA in comparison with the control shRNA cells. Flow cytometry analysis showed that PPM1A shRNA significantly increased the percentage of apoptotic BV2 cells infected with B. suis strain 2 compared with those transfected with control shRNA. Taken together, these data suggested that knockdown of PPM1A promotes apoptosis in B. suis strain 2‑infected BV2 cells and that PPM1A may be a potential target in the development of treatments to inhibit intracellular growth of B. suis.
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1 

Vollmar P, Zange S, Zöller L, Erkel J and Robert Thoma B: Brucellosis, an overview and current aspects. Dtsch Med Wochenschr. 141:1014–1018. 2016.PubMed/NCBI View Article : Google Scholar : (In German).

2 

Zheng R, Xie S, Lu X, Sun L, Zhou Y, Zhang Y and Wang K: A systematic review and meta-analysis of epidemiology and clinical manifestations of human brucellosis in China. Biomed Res Int. 2018(5712920)2018.PubMed/NCBI View Article : Google Scholar

3 

Galinska EM and Zagorski J: Brucellosis in humans-etiology, diagnostics, clinical forms. Ann Agric Environ Med. 20:233–238. 2013.PubMed/NCBI

4 

Pelerito A, Cordeiro R, Matos R, Santos MA, Soeiro S, Santos J, Manita C, Rio C, Santo M, Paixão E, et al: Human brucellosis in portugal-retrospective analysis of suspected clinical cases of infection from 2009 to 2016. PLoS One. 12(e0179667)2017.PubMed/NCBI View Article : Google Scholar

5 

Elfaki MG, Alaidan AA and Al-Hokail AA: Host response to brucella infection: Review and future perspective. J Infect Dev Ctries. 9:697–701. 2015.PubMed/NCBI View Article : Google Scholar

6 

Miller CN, Smith EP, Cundiff JA, Knodler LA, Bailey Blackburn J, Lupashin V and Celli J: A brucella type iv effector targets the cog tethering complex to remodel host secretory traffic and promote intracellular replication. Cell Host Microbe. 22:317–329. 2017.PubMed/NCBI View Article : Google Scholar

7 

Carvalho TF, Haddad JP, Paixão TA and Santos RL: Meta-Analysis and advancement of brucellosis vaccinology. PLoS One. 11(e0166582)2016.PubMed/NCBI View Article : Google Scholar

8 

Schaaf K, Smith SR, Duverger A, Wagner F, Wolschendorf F, Westfall AO, Kutsch O and Sun J: Mycobacterium tuberculosis exploits the PPM1A signaling pathway to block host macrophage apoptosis. Sci Rep. 7(42101)2017.PubMed/NCBI View Article : Google Scholar

9 

Sun J, Schaaf K, Duverger A, Wolschendorf F, Speer A, Wagner F, Niederweis M and Kutsch O: Protein phosphatase, Mg2+/Mn2+-dependent 1A controls the innate antiviral and antibacterial response of macrophages during HIV-1 and mycobacterium tuberculosis infection. Oncotarget. 7:15394–15409. 2016.PubMed/NCBI View Article : Google Scholar

10 

Xiang W, Zhang Q, Lin X, Wu S, Zhou Y, Meng F, Fan Y, Shen T, Xiao M, Xia Z, et al: PPM1A silences cytosolic RNA sensing and antiviral defense through direct dephosphorylation of MAVS and TBK1. Sci Adv. 2(e1501889)2016.PubMed/NCBI View Article : Google Scholar

11 

Siqueira MDS, Ribeiro RM and Travassos LH: Autophagy and its interaction with intracellular bacterial pathogens. Front Immunol. 9(935)2018.PubMed/NCBI View Article : Google Scholar

12 

Li Y, Wei C, Xu H, Jia J, Wei Z, Guo R, Jia Y, Wu Y, Li Y, Qi X, et al: The immunoregulation of Th17 in host against intracellular bacterial infection. Mediators Inflamm. 19(6587296)2018.PubMed/NCBI View Article : Google Scholar

13 

Heppner FL, Ransohoff RM and Becher B: Immune attack: The role of inflammation in alzheimer disease. Nat Rev Neurosci. 16:358–372. 2015.PubMed/NCBI View Article : Google Scholar

14 

Yang J, Zhang J, Xu T, Wang Y and Wang Z: Establishment of BV2 cell line with steady knockdown of Mg2+/Mn2+-dependent protein phosphatase 1A(PPM1A). Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 34:818–823. 2018.PubMed/NCBI(In Chinese).

15 

Ma QL, Liu AC, Ma XJ, Wang YB, Hou YT and Wang ZH: Brucella outer membrane protein Omp25 induces microglial cells in vitro to secrete inflammatory cytokines and inhibit apoptosis. Int J Clin Exp Med. 8:17530–17535. 2015.PubMed/NCBI

16 

Smith SR, Schaaf K, Rajabalee N, Wagner F, Duverger A, Kutsch O and Sun J: The phosphatase PPM1A controls monocyte-to-macrophage differentiation. Sci Rep. 8(902)2018.PubMed/NCBI View Article : Google Scholar

17 

Wang Y, Dow EC, Liang YY, Ramakrishnan R, Liu H, Sung TL, Lin X and Rice AP: Phosphatase PPM1A regulates phosphorylation of thr-186 in the Cdk9 T-loop. J Biol Chem. 283:33578–22584. 2008.PubMed/NCBI View Article : Google Scholar

18 

Sun W, Yu Y, Dotti G, Shen T, Tan X, Savoldo B, Pass AK, Chu M, Zhang D, Lu X, et al: PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation. Cell Signal. 21:95–102. 2009.PubMed/NCBI View Article : Google Scholar

19 

Levy J, Shneck M, Marcus M and Lifshitz T: Brucella meningitis and papilledema in a child. Eur J Ophthalmol. 15:818–820. 2005.PubMed/NCBI View Article : Google Scholar

20 

Haji-Abdolbagi M, Rasooli-Nejad M, Jafari S, Hasibi M and Soudbakhsh A: Clinical and laboratory findings in neurobrucellosis: Review of 31 cases. Arch Iran Med. 11:21–25. 2008.PubMed/NCBI

21 

Li YJ, Li XL, Liang S, Fang LQ and Cao WC: Epidemiological features and risk factors associated with the spatial and temporal distribution of human brucellosis in China. BMC Infect Dis. 13(547)2013.PubMed/NCBI View Article : Google Scholar

22 

Lai S, Zhou H, Xiong W, Gilbert M, Huang Z, Yu J, Yin W, Wang L, Chen Q, Li Y, et al: Changing epidemiology of human brucellosis, China, 1955-2014. Emerg Infect Dis. 23:184–194. 2017.PubMed/NCBI View Article : Google Scholar

23 

Deng Y, Liu X, Duan K and Peng Q: Research progress on brucellosis. Curr Med Chem. 26:5598–5608. 2018.

24 

Tu L, Liu X, Gu W, Wang Z, Zhang E, Kahar A, Chu G and Zhao J: Imaging-assisted diagnosis and characteristics of suspected spinal brucellosis: A retrospective study of 72 cases. Med Sci Monit. 24:2647–2654. 2018.PubMed/NCBI View Article : Google Scholar

25 

Sabzi F and Faraji R: Brucella pericarditis: A forgotten cause of chest pain. Caspian J Intern Med. 8:116–118. 2017.PubMed/NCBI View Article : Google Scholar

26 

Olsen SC and Palmer MV: Advancement of knowledge of brucella over the past 50 years. Vet Pathol. 51:1076–1089. 2014.PubMed/NCBI View Article : Google Scholar

27 

Moreno E and Moriyon I: Brucella melitensis: A nasty bug with hidden credentials for virulence. Proc Natl Acad Sci USA. 99:1–3. 2002.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yang J, Wang G, Li H, Zheng W, Guo B and Wang Z: Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. Exp Ther Med 20: 926-932, 2020.
APA
Yang, J., Wang, G., Li, H., Zheng, W., Guo, B., & Wang, Z. (2020). Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. Experimental and Therapeutic Medicine, 20, 926-932. https://doi.org/10.3892/etm.2020.8745
MLA
Yang, J., Wang, G., Li, H., Zheng, W., Guo, B., Wang, Z."Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine". Experimental and Therapeutic Medicine 20.2 (2020): 926-932.
Chicago
Yang, J., Wang, G., Li, H., Zheng, W., Guo, B., Wang, Z."Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine". Experimental and Therapeutic Medicine 20, no. 2 (2020): 926-932. https://doi.org/10.3892/etm.2020.8745
Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Wang G, Li H, Zheng W, Guo B and Wang Z: Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. Exp Ther Med 20: 926-932, 2020.
APA
Yang, J., Wang, G., Li, H., Zheng, W., Guo, B., & Wang, Z. (2020). Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. Experimental and Therapeutic Medicine, 20, 926-932. https://doi.org/10.3892/etm.2020.8745
MLA
Yang, J., Wang, G., Li, H., Zheng, W., Guo, B., Wang, Z."Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine". Experimental and Therapeutic Medicine 20.2 (2020): 926-932.
Chicago
Yang, J., Wang, G., Li, H., Zheng, W., Guo, B., Wang, Z."Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine". Experimental and Therapeutic Medicine 20, no. 2 (2020): 926-932. https://doi.org/10.3892/etm.2020.8745
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