Open Access

Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice

  • Authors:
    • Gang Liu
    • Fengwei Yin
    • Congli Zhang
    • Yang Zhang
    • Xiaohong Li
    • Yunzhi Ling
  • View Affiliations

  • Published online on: May 19, 2020     https://doi.org/10.3892/etm.2020.8768
  • Pages: 1191-1197
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aimƒ of this study was to explore effects of miR-132 and glycogen synthase kinase-3β (GSK-3β) on learning and memory in mice. miR-132 inhibitor GSK-3β overexpression agent (sh-GSK-3β) and normal saline (negative control group) were injected into the hippocampus of adult mice, and healthy adult mice were taken as the unrelated control group. The expression of miR-132 and GSK-3β in the hippocampus of adult and elderly mice was detected using reverse transcription-quantitative PCR (RT-qPCR) and western blot analysis. Morris water maze test was employed to detect learning and memory function in mice. The dual luciferase reporter was adopted to determine the relationship between miR-132 and GSK-3β. Compared with the adult group, the expression of miR-132 was significantly downregulated in the hippocampus in the elderly group, while the expression of GSK-3β was upregulated. Injecting miR-132 inhibitor into the hippocampus of adult mice led to a significant increase in escape latency and a significant decrease in the number of times of crossing platforms. The injection of GSK-3β overexpression agent into the hippocampus of adult mice resulted in a marked increase in escape latency and a significant decrease in the number of times of crossing platforms in the water maze test. It was also found that downregulation of GSK-3β reversed the decline in learning and memory in mice caused by downregulation of miR-132 expression. The dual luciferase report identified a targeted regulatory relationship between miR-132 and GSK-3β. Overexpression of miR-132 can inhibit the expression of GSK-3β in mouse learning and memory ability, which provides some inspiration for understanding the occurrence of learning and memory disorders and future treatment methods.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu G, Yin F, Zhang C, Zhang Y, Li X and Ling Y: Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice. Exp Ther Med 20: 1191-1197, 2020
APA
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., & Ling, Y. (2020). Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice. Experimental and Therapeutic Medicine, 20, 1191-1197. https://doi.org/10.3892/etm.2020.8768
MLA
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., Ling, Y."Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice". Experimental and Therapeutic Medicine 20.2 (2020): 1191-1197.
Chicago
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., Ling, Y."Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1191-1197. https://doi.org/10.3892/etm.2020.8768