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Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice

  • Authors:
    • Gang Liu
    • Fengwei Yin
    • Congli Zhang
    • Yang Zhang
    • Xiaohong Li
    • Yunzhi Ling
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233003, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1191-1197
    |
    Published online on: May 19, 2020
       https://doi.org/10.3892/etm.2020.8768
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Abstract

The aimƒ of this study was to explore effects of miR-132 and glycogen synthase kinase-3β (GSK-3β) on learning and memory in mice. miR-132 inhibitor GSK-3β overexpression agent (sh-GSK-3β) and normal saline (negative control group) were injected into the hippocampus of adult mice, and healthy adult mice were taken as the unrelated control group. The expression of miR-132 and GSK-3β in the hippocampus of adult and elderly mice was detected using reverse transcription-quantitative PCR (RT-qPCR) and western blot analysis. Morris water maze test was employed to detect learning and memory function in mice. The dual luciferase reporter was adopted to determine the relationship between miR-132 and GSK-3β. Compared with the adult group, the expression of miR-132 was significantly downregulated in the hippocampus in the elderly group, while the expression of GSK-3β was upregulated. Injecting miR-132 inhibitor into the hippocampus of adult mice led to a significant increase in escape latency and a significant decrease in the number of times of crossing platforms. The injection of GSK-3β overexpression agent into the hippocampus of adult mice resulted in a marked increase in escape latency and a significant decrease in the number of times of crossing platforms in the water maze test. It was also found that downregulation of GSK-3β reversed the decline in learning and memory in mice caused by downregulation of miR-132 expression. The dual luciferase report identified a targeted regulatory relationship between miR-132 and GSK-3β. Overexpression of miR-132 can inhibit the expression of GSK-3β in mouse learning and memory ability, which provides some inspiration for understanding the occurrence of learning and memory disorders and future treatment methods.
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1 

Lin S and Gregory RI: MicroRNA biogenesis pathways in cancer. Nat Rev Cancer. 15:321–333. 2015.PubMed/NCBI View Article : Google Scholar

2 

Agarwal V, Bell GW, Nam JW and Bartel DP: Predicting effective microRNA target sites in mammalian mRNAs. eLife. 4(e05005)2015.PubMed/NCBI View Article : Google Scholar

3 

Qian Y, Song J, Ouyang Y, Han Q, Chen W, Zhao X, Xie Y, Chen Y, Yuan W and Fan C: Advances in roles of miR-132 in the nervous system. Front Pharmacol. 8(770)2017.PubMed/NCBI View Article : Google Scholar

4 

Wei CW, Luo T, Zou SS and Wu AS: Research progress on the roles of microRNAs in governing synaptic plasticity, learning and memory. Life Sci. 188:118–122. 2017.PubMed/NCBI View Article : Google Scholar

5 

Wang Y, Veremeyko T, Wong AHK, El Fatimy R, Wei Z, Cai W and Krichevsky AM: Downregulation of miR-132/212 impairs S-nitrosylation balance and induces tau phosphorylation in Alzheimer's disease. Neurobiol Aging. 51:156–166. 2017.PubMed/NCBI View Article : Google Scholar

6 

Liu Y, Yang X, Zhao L, Zhang J, Li T and Ma X: Increased miR-132 level is associated with visual memory dysfunction in patients with depression. Neuropsychiatr Dis Treat. 12:2905–2911. 2016.PubMed/NCBI View Article : Google Scholar

7 

Guo Y, Gupte M, Umbarkar P, Singh AP, Sui JY, Force T and Lal H: Entanglement of GSK-3β, β-catenin and TGF-β1 signaling network to regulate myocardial fibrosis. J Mol Cell Cardiol. 110:109–120. 2017.PubMed/NCBI View Article : Google Scholar

8 

Zhang H, Yang X, Qin X and Niu Q: Caspase-3 is involved in aluminum-induced impairment of long-term potentiation in rats through the Akt/GSK-3β pathway. Neurotox Res. 29:484–494. 2016.PubMed/NCBI View Article : Google Scholar

9 

Lu F, Li X, Li W, Wei K, Yao Y, Zhang Q, Liang X and Zhang J: Tetramethylpyrazine reverses intracerebroventricular streptozotocin-induced memory deficits by inhibiting GSK-3β. Acta Biochim Biophys Sin (Shanghai). 49:722–728. 2017.PubMed/NCBI View Article : Google Scholar

10 

Suh JH, Choi E, Cha MJ, Song BW, Ham O, Lee SY, Yoon C, Lee CY, Park JH, Lee SH, et al: Up-regulation of miR-26a promotes apoptosis of hypoxic rat neonatal cardiomyocytes by repressing GSK-3β protein expression. Biochem Biophys Res Commun. 423:404–410. 2012.PubMed/NCBI View Article : Google Scholar

11 

Tsukigi M, Bilim V, Yuuki K, Ugolkov A, Naito S, Nagaoka A, Kato T, Motoyama T and Tomita Y: Re-expression of miR-199a suppresses renal cancer cell proliferation and survival by targeting GSK-3β. Cancer Lett. 315:189–197. 2012.PubMed/NCBI View Article : Google Scholar

12 

Wolfensohn S and Lloyd M: Handbook of laboratory animal management and welfare. John Wiley & Sons. 2008. View Article : Google Scholar

13 

Rupaimoole R and Slack FJ: MicroRNA therapeutics: Towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 16:203–222. 2017.PubMed/NCBI View Article : Google Scholar

14 

Busto GU, Guven-Ozkan T, Chakraborty M and Davis RL: Developmental inhibition of miR-iab8-3p disrupts mushroom body neuron structure and adult learning ability. Dev Biol. 419:237–249. 2016.PubMed/NCBI View Article : Google Scholar

15 

Xu XF, Jing X, Ma HX, Yuan RR, Dong Q, Dong JL, Han XF, Chen ZY, Li XZ and Wang Y: miR-181a participates in contextual fear memory formation via activating mTOR signaling pathway. Cereb Cortex. 28:3309–3321. 2018.PubMed/NCBI View Article : Google Scholar

16 

Tian F, Yuan C and Yue H: MiR-138/SIRT1 axis is implicated in impaired learning and memory abilities of cerebral ischemia/reperfusion injured rats. Exp Cell Res. 367:232–240. 2018.PubMed/NCBI View Article : Google Scholar

17 

Wang RY, Phang RZ, Hsu PH, Wang WH, Huang HT and Liu IY: In vivo knockdown of hippocampal miR-132 expression impairs memory acquisition of trace fear conditioning. Hippocampus. 23:625–633. 2013. View Article : Google Scholar

18 

Huang S, Zhao J, Huang D, Zhuo L, Liao S and Jiang Z: Serum miR-132 is a risk marker of post-stroke cognitive impairment. Neurosci Lett. 615:102–106. 2016.PubMed/NCBI View Article : Google Scholar

19 

Numakawa T, Yamamoto N, Chiba S, Richards M, Ooshima Y, Kishi S, Hashido K, Adachi N and Kunugi H: Growth factors stimulate expression of neuronal and glial miR-132. Neurosci Lett. 505:242–247. 2011.PubMed/NCBI View Article : Google Scholar

20 

Magill ST, Cambronne XA, Luikart BW, Lioy DT, Leighton BH, Westbrook GL, Mandel G and Goodman RH: microRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus. Proc Natl Acad Sci USA. 107:20382–20387. 2010.PubMed/NCBI View Article : Google Scholar

21 

Zhang S, Liang Z, Sun W and Pei L: Repeated propofol anesthesia induced downregulation of hippocampal miR-132 and learning and memory impairment of rats. Brain Res. 1670:156–164. 2017.PubMed/NCBI View Article : Google Scholar

22 

Hernandez-Rapp J, Smith PY, Filali M, Goupil C, Planel E, Magill ST, Goodman RH and Hébert SS: Memory formation and retention are affected in adult miR-132/212 knockout mice. Behav Brain Res. 287:15–26. 2015.PubMed/NCBI View Article : Google Scholar

23 

Sun A, Li C, Chen R, Huang Y, Chen Q, Cui X, Liu H, Thrasher JB and Li B: GSK-3β controls autophagy by modulating LKB1-AMPK pathway in prostate cancer cells. Prostate. 76:172–183. 2016.PubMed/NCBI View Article : Google Scholar

24 

Hong JG, Kim DH, Lee CH, Park SJ, Kim JM, Cai M, Jang DS and Ryu JH: GSK-3β activity in the hippocampus is required for memory retrieval. Neurobiol Learn Mem. 98:122–129. 2012.PubMed/NCBI View Article : Google Scholar

25 

Hui J, Zhang J, Pu M, Zhou X, Dong L, Mao X, Shi G, Zou J, Wu J, Jiang D, et al: Modulation of GSK-3β/β-catenin signaling contributes to learning and memory impairment in a rat model of depression. Int J Neuropsychopharmacol. 21:858–870. 2018.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Liu G, Yin F, Zhang C, Zhang Y, Li X and Ling Y: Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice. Exp Ther Med 20: 1191-1197, 2020.
APA
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., & Ling, Y. (2020). Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice. Experimental and Therapeutic Medicine, 20, 1191-1197. https://doi.org/10.3892/etm.2020.8768
MLA
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., Ling, Y."Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice". Experimental and Therapeutic Medicine 20.2 (2020): 1191-1197.
Chicago
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., Ling, Y."Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1191-1197. https://doi.org/10.3892/etm.2020.8768
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, Yin F, Zhang C, Zhang Y, Li X and Ling Y: Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice. Exp Ther Med 20: 1191-1197, 2020.
APA
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., & Ling, Y. (2020). Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice. Experimental and Therapeutic Medicine, 20, 1191-1197. https://doi.org/10.3892/etm.2020.8768
MLA
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., Ling, Y."Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice". Experimental and Therapeutic Medicine 20.2 (2020): 1191-1197.
Chicago
Liu, G., Yin, F., Zhang, C., Zhang, Y., Li, X., Ling, Y."Effects of regulating miR-132 mediated GSK-3β on learning and memory function in mice". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1191-1197. https://doi.org/10.3892/etm.2020.8768
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