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STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF

  • Authors:
    • Yanjiao Huang
    • Qiang Li
    • Zunyong Feng
    • Lanrong Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Pathological Anatomy, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Department of Anatomy, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Department of Forensic Medicine, Wannan Medical College, Wuhu, Anhui 241002, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 736-747
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    Published online on: May 19, 2020
       https://doi.org/10.3892/etm.2020.8774
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Abstract

Store-operated Ca2+ entry (SOCE) is the stable calcium channel influx in most cells. It consists of the cytoplasmic ion channel ORAI and endoplasmic reticulum receptor stromal interaction molecule 1 (STIM1). Abolition of SOCE function due to ORAI1 and STIM1 gene defects may cause non‑perspiration, ectoderm dysplasia and skeletal malformations with severe combined immunodeficiency (CID). Calcineurin/mammalian target of rapamycin (mTOR)/nuclear factor of activated T cells 2 (NFATC2) is an important signalling cascade for osteoclast development. Calcineurin is activated by Ca2+ via SOCE during osteoclastogenesis, which is induced by receptor activator of NF‑κB ligand (RANKL) and macrophage colony‑stimulating factor (M‑CSF). However, the underlying mechanism has remained to be fully elucidated, which was therefore the aim of the present study. In the current study, flow cytometry was used to examine the effect of a number of STIM1 mutations on proliferation, differentiation, and expression of osteolysis‑associated proteins in Bone marrow‑derived mononuclear macrophages (BMDM). The calcineurin/AKT/mTOR/NFATC2 signaling cascade activation were also assessed. BMDMs were obtained from three patients with STIM1 mutations (p.E136X, p.R429C and p.R304W). These mutations, which exhibited abolished (p.E136X, p.R429C) or constitutively activated (p.R304W) SOCE, failed to respond to RANKL/M‑CSF‑mediated induction of normal osteoclastogenesis. In addition, activation of the calcineurin/Akt/mTOR/NFATC2 signalling cascade induced by RANKL/M‑CSF was abnormal in the BMDMs with STIM1 mutants compared with that in BMDMs from healthy subjects. In addition, overexpression of wild‑type STIM1 restored SOCE in p.R429C‑ and p.E136X‑mutant BMDMs, but not in p.R304W‑mutant BMDMs. Of note, calcineurin, cyclosporin A, mTOR inhibitor rapamycin and NFATC2‑specific small interfering RNA restored the function of SOCE in p.R304W‑mutant BMDMs. The present study suggests a role for SOCE in calcineurin/Akt/mTOR/NFATC2‑mediated osteoclast proliferation, differentiation and function.
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View References

1 

Boyle WJ, Simonet WS and Lacey DL: Osteoclast differentiation and activation. Nature. 423:337–342. 2003.PubMed/NCBI View Article : Google Scholar

2 

Harada S and Rodan GA: Control of osteoblast function and regulation of bone mass. Nature. 423:349–355. 2003.PubMed/NCBI View Article : Google Scholar

3 

Zaidi M: Skeletal remodeling in health and disease. Nat Med. 13:791–801. 2007.PubMed/NCBI View Article : Google Scholar

4 

Kobayashi E and Setsu N: Osteosclerosis induced by denosumab. Lancet. 7(539)2015.PubMed/NCBI View Article : Google Scholar

5 

Luo J, Yang Z, Ma Y, Yue Z, Lin H, Qu G, Huang J, Dai W, Li C, Zheng C, et al: LGR4 is a receptor for RANKL and negatively regulates osteoclast differentiation and bone resorption. Nat Med. 22:539–546. 2016.PubMed/NCBI View Article : Google Scholar

6 

Fu J, Li S, Feng R, Ma H, Sabeh F, Roodman GD, Wang J, Robinson S, Guo XE, Lund T, et al: Multiple myeloma-derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease. J Clin Invest. 126:1759–1772. 2016.PubMed/NCBI View Article : Google Scholar

7 

Okamoto K, Nakashima T, Shinohara M, Negishi-Koga T, Komatsu N, Terashima A, Sawa S, Nitta T and Takayanagi H: Osteoimmunology: The conceptual framework unifying the immune and skeletal systems. Physiol Rev. 97:1295–1349. 2017.PubMed/NCBI View Article : Google Scholar

8 

Chen X, Zhi X, Cao L, Weng W, Pan P, Hu H, Liu C, Zhao Q, Zhou Q, Cui J and Su J: Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis. Cell Death Dis. 8(e3037)2017.PubMed/NCBI View Article : Google Scholar

9 

Suematsu A, Nakashima T, Takemoto-Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, et al: Regulation of osteoclast differentiation and function by the CaMK-CREB pathway. Nat Med. 17:1410–1416. 2006.PubMed/NCBI View Article : Google Scholar

10 

Ortuño MJ, Robinson ST, Subramanyam P, Paone R, Huang YY, Guo XE, Colecraft HM, Mann JJ and Ducy P: Serotonin-reuptake inhibitors act centrally to cause bone loss in mice by counteracting a local anti-resorptive effect. Nat Med. 22:1170–1179. 2016.PubMed/NCBI View Article : Google Scholar

11 

Xu S, Zhang Y, Wang J, Li K, Tan K, Liang K, Shen J, Cai D, Jin D, Li M, et al: TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42. Cell Death Differ. 25:1549–1566. 2018.PubMed/NCBI View Article : Google Scholar

12 

Hoefle G, Holzmueller H and Drexel H: Alendronate versus calcitriol for prevention of bone loss after cardiac transplantation. N Engl J Med. 350:2306–2308. 2004.PubMed/NCBI

13 

Janssen NM and Genta MS: The effects of immunosuppressive and anti-inflammatory medications on fertility, pregnancy, and lactation. Arch Intern Med. 160:610–619. 2000.PubMed/NCBI View Article : Google Scholar

14 

Huynh H, Wei W and Wan Y: mTOR inhibition subdues milk disorder caused by maternal VLDLR loss. Cell Rep. 19:2014–2025. 2017.PubMed/NCBI View Article : Google Scholar

15 

Zhou C, You Y, Shen W, Zhu YZ, Peng J, Feng HT, Wang Y, Li D, Shao WW, Li CX, et al: Deficiency of sorting nexin 10 prevents bone erosion in collagen-induced mouse arthritis through promoting NFATc1 degradation. Ann Rheum Dis. 75:1211–1218. 2016.PubMed/NCBI View Article : Google Scholar

16 

Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, et al: Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 3:889–901. 2002.PubMed/NCBI View Article : Google Scholar

17 

Kim H, Kim T, Jeong BC, Cho IT, Han D, Takegahara N, Negishi-Koga T, Takayanagi H, Lee JH, Sul JY, et al: Tmem64 modulates calcium signaling during RANKL-mediated osteoclast differentiation. Cell Metab. 17:249–260. 2013.PubMed/NCBI View Article : Google Scholar

18 

Masuyama R, Vriens J, Voets T, Karashima Y, Owsianik G, Vennekens R, Lieben L, Torrekens S, Moermans K, Vanden Bosch A, et al: TRPV4-mediated calcium influx regulates terminal differentiation of osteoclasts. Cell Metab. 8:257–265. 2008.PubMed/NCBI View Article : Google Scholar

19 

Hwang SY and Putney JW: Orai1-mediated calcium entry plays a critical role in osteoclast differentiation and function by regulating activation of the transcription factor NFATc1. FASEB J. 26:1484–1492. 2012.PubMed/NCBI View Article : Google Scholar

20 

Crabtree NJ, Shaw NJ, Bishop NJ, Adams JE, Mughal MZ, Arundel P, Fewtrell MS, Ahmed SF, Treadgold LA, Högler W, et al: Amalgamated reference data for size-adjusted bone densitometry measurements in 3598 children and young adults-the ALPHABET study. J Bone Miner Res. 32:172–180. 2017.PubMed/NCBI View Article : Google Scholar

21 

Guo B, Xu Y, Gong J, Tang Y and Xu H: Age trends of bone mineral density and percentile curves in healthy Chinese children and adolescents. J Bone Miner Metab. 31:304–314. 2013.PubMed/NCBI View Article : Google Scholar

22 

Notredame C, Higgins DG and Heringa J: T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol. 302:205–217. 2000.PubMed/NCBI View Article : Google Scholar

23 

Huang Z, Ruan HB, Xian L, Chen W, Jiang S, Song A, Wang Q, Shi P, Gu X and Gao X: The stem cell factor/Kit signalling pathway regulates mitochondrial function and energy expenditure. Nat Commun. 5(4282)2014.PubMed/NCBI View Article : Google Scholar

24 

Misceo D, Holmgren A, Louch WE, Holme PA, Mizobuchi M, Morales RJ, De Paula AM, Stray-Pedersen A, Lyle R, Dalhus B, et al: A dominant STIM1 mutation causes Stormorken syndrome. Hum Mutat. 35:556–564. 2014.PubMed/NCBI View Article : Google Scholar

25 

Picard C, Mccarl CA, Papolos A, Khalil S, Lüthy K, Hivroz C, LeDeist F, Rieux-Laucat F, Rechavi G, Rao A, et al: STIM1 mutation associated with a syndrome of immunodeficiency and autoimmunity. N Engl J Med. 360:1971–1980. 2009.PubMed/NCBI View Article : Google Scholar

26 

Maus M, Jairaman A, Stathopulos PB, Muik M, Fahrner M, Weidinger C, Benson M, Fuchs S, Ehl S, Romanin C, et al: Missense mutation in immunodeficient patients shows the multifunctional roles of coiled-coil domain 3 (CC3) in STIM1 activation. Proc Natl Acad Sci USA. 112:6206–6211. 2015.PubMed/NCBI View Article : Google Scholar

27 

Morin G, Bruechle NO, Singh AR, Knopp C, Jedraszak G, Elbracht M, Brémond-Gignac D, Hartmann K, Sevestre H, Deutz P, et al: Gain-of-function mutation in STIM1 (P.R304W) is associated with Stormorken syndrome. Hum Mutat. 35:1221–1232. 2015.PubMed/NCBI View Article : Google Scholar

28 

Vaeth M, Maus M, Klein-Hessling S, Freinkman E, Yang J, Eckstein M, Cameron S, Turvey SE, Serfling E, Berberich-Siebelt F, et al: Store-operated Ca2+ entry controls clonal expansion of T cells through metabolic reprogramming. Immunity. 47:664–679.e6. 2017.PubMed/NCBI View Article : Google Scholar

29 

Li CH, Yang ZF, Li ZX, Ma Y, Zhang L, Zheng C, Qiu W, Wu X, Wang X, Li H, et al: Maslinic acid suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss by regulating RANKL-mediated NF-κB and MAPK signaling pathways. J Bone Miner Res. 26:644–656. 2011.PubMed/NCBI View Article : Google Scholar

30 

Ohta K, Naruse T, Ishida Y, Shigeishi H, Nakagawa T, Fukui A, Nishi H, Sasaki K, Ogawa I and Takechi M: TNF-α-induced IL-6 and MMP-9 expression in immortalized ameloblastoma cell line established by hTERT. Oral Dis. 23:199–209. 2017.PubMed/NCBI View Article : Google Scholar

31 

Huynh H and Wan Y: mTORC1 impedes osteoclast differentiation via calcineurin and NFATc1. Commun Biol. 1(29)2018.PubMed/NCBI View Article : Google Scholar

32 

Hogan PG, Chen L, Nardone J and Rao A: Transcriptional regulation by calcium, calcineurin, and NFAT. Genes Dev. 15:2205–2232. 2003.PubMed/NCBI View Article : Google Scholar

33 

Cahalan MD: STIMulating store-operated Ca(2+) entry. Nat Cell Biol. 11:669–677. 2009.PubMed/NCBI View Article : Google Scholar

34 

Lewis RS: The molecular choreography of a store-operated calcium channel. Nature. 446:284–287. 2007.PubMed/NCBI View Article : Google Scholar

35 

Roos J, DiGregorio PJ, Yeromin AV, Ohlsen K, Lioudyno M, Zhang S, Safrina O, Kozak JA, Wagner SL, Cahalan MD, et al: STIM1, an essential and conserved component of store-operated Ca2+ channel function. J Cell Biol. 169:435–445. 2005.PubMed/NCBI View Article : Google Scholar

36 

Feske S, Gwack Y, Prakriya M, Srikanth S, Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M and Rao A: A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 441:179–185. 2006.PubMed/NCBI View Article : Google Scholar

37 

Vig M, Peinelt C, Beck A, Koomoa DL, Rabah D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R and Kinet JP: CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science. 312:1220–1223. 2006.PubMed/NCBI View Article : Google Scholar

38 

Zhang SL, Yeromin AV, Zhang XH, Yu Y, Safrina O, Penna A, Roos J, Stauderman KA and Cahalan MD: Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. Proc Natl Acad Sci USA. 103:9357–9362. 2006.PubMed/NCBI View Article : Google Scholar

39 

Feske S, Skolnik EY and Prakriya M: Ion channels and transporters in lymphocyte function and immunity. Nat Rev Immunol. 12:532–547. 2012.PubMed/NCBI View Article : Google Scholar

40 

Parekh AB: Store-operated CRAC channels: Function in health and disease. Nat Rev Drug Discov. 9:399–410. 2010.PubMed/NCBI View Article : Google Scholar

41 

Wang L, Iorio C, Yan K, Yang H, Takeshita S, Kang S, Neel BG and Yang W: A ERK/RSK-mediated negative feedback loop regulates M-CSF-evoked PI3K/AKT activation in macrophages. FASEB J. 32:875–887. 2018.PubMed/NCBI View Article : Google Scholar

42 

Pacitto R, Gaeta I, Swanson JA and Yoshida S: CXCL12-induced macropinocytosis modulates two distinct pathways to activate mTORC1 in macrophages. J Leukoc Biol. 101:683–692. 2017.PubMed/NCBI View Article : Google Scholar

43 

Walsh MC and Choi Y: Biology of the RANKL-RANK-OPG system in immunity, bone, and beyond. Front Immunol. 5(511)2014.PubMed/NCBI View Article : Google Scholar

44 

Dowling RJ, Topisirovic I, Fonseca BD and Sonenberg N: Dissecting the role of mTOR: Lessons from mTOR inhibitors. Biochim Biophys Acta. 1804:433–439. 2010.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Huang Y, Li Q, Feng Z and Zheng L: STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF. Exp Ther Med 20: 736-747, 2020.
APA
Huang, Y., Li, Q., Feng, Z., & Zheng, L. (2020). STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF. Experimental and Therapeutic Medicine, 20, 736-747. https://doi.org/10.3892/etm.2020.8774
MLA
Huang, Y., Li, Q., Feng, Z., Zheng, L."STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF". Experimental and Therapeutic Medicine 20.2 (2020): 736-747.
Chicago
Huang, Y., Li, Q., Feng, Z., Zheng, L."STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF". Experimental and Therapeutic Medicine 20, no. 2 (2020): 736-747. https://doi.org/10.3892/etm.2020.8774
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Li Q, Feng Z and Zheng L: STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF. Exp Ther Med 20: 736-747, 2020.
APA
Huang, Y., Li, Q., Feng, Z., & Zheng, L. (2020). STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF. Experimental and Therapeutic Medicine, 20, 736-747. https://doi.org/10.3892/etm.2020.8774
MLA
Huang, Y., Li, Q., Feng, Z., Zheng, L."STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF". Experimental and Therapeutic Medicine 20.2 (2020): 736-747.
Chicago
Huang, Y., Li, Q., Feng, Z., Zheng, L."STIM1 controls calcineurin/Akt/mTOR/NFATC2‑mediated osteoclastogenesis induced by RANKL/M‑CSF". Experimental and Therapeutic Medicine 20, no. 2 (2020): 736-747. https://doi.org/10.3892/etm.2020.8774
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