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Article

MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5

  • Authors:
    • Na Yue
    • Ming Ye
    • Ran Zhang
    • Miao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Third Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China, Department of Pathology, Occupational Disease Hospital, Urumqi, Xinjiang Uygur Autonomous Region 830091, P.R. China
  • Pages: 1746-1751
    |
    Published online on: May 28, 2020
       https://doi.org/10.3892/etm.2020.8814
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Abstract

The aim of the present study was to explore the roles of microRNA‑1307‑3p (miR‑1307‑3p) in colorectal cancer (CRC). Firstly, the expression level of miR‑1307‑3p in CRC cells was measured using reverse transcription‑quantitative PCR. Subsequently, Cell Counting Kit‑8 and Transwell invasion assays were performed to evaluate the effects of miR‑1307‑3p on CRC cell proliferation and invasion, respectively. Bioinformatics tools and dual luciferase reporter assays were used to validate the targets of miR‑1307‑3p. Rescue experiments were performed to confirm tumor suppressor candidate 5 (TUSC5) as a functional target of miR‑1307‑3p. miR‑1307‑3p levels were revealed to be upregulated in CRC cells when compared with the normal human epithelial cell line. Knockdown of miR‑1307‑3p inhibited CRC cell growth and invasiveness. Bioinformatics analysis and dual‑luciferase activity reporter assays demonstrated that miR‑1307‑3p binds the 3'‑untranslated region of TUSC5. Finally, rescue experiments validated that miR‑1307‑3p was able to regulate CRC cell behaviors via regulating TUSC5 expression. Together, the current results indicate that miR‑1307‑3p functions as an oncogenic miRNA via targeting TUSC5 in CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Yue N, Ye M, Zhang R and Wang M: MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5. Exp Ther Med 20: 1746-1751, 2020.
APA
Yue, N., Ye, M., Zhang, R., & Wang, M. (2020). MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5. Experimental and Therapeutic Medicine, 20, 1746-1751. https://doi.org/10.3892/etm.2020.8814
MLA
Yue, N., Ye, M., Zhang, R., Wang, M."MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5". Experimental and Therapeutic Medicine 20.2 (2020): 1746-1751.
Chicago
Yue, N., Ye, M., Zhang, R., Wang, M."MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1746-1751. https://doi.org/10.3892/etm.2020.8814
Copy and paste a formatted citation
x
Spandidos Publications style
Yue N, Ye M, Zhang R and Wang M: MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5. Exp Ther Med 20: 1746-1751, 2020.
APA
Yue, N., Ye, M., Zhang, R., & Wang, M. (2020). MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5. Experimental and Therapeutic Medicine, 20, 1746-1751. https://doi.org/10.3892/etm.2020.8814
MLA
Yue, N., Ye, M., Zhang, R., Wang, M."MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5". Experimental and Therapeutic Medicine 20.2 (2020): 1746-1751.
Chicago
Yue, N., Ye, M., Zhang, R., Wang, M."MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1746-1751. https://doi.org/10.3892/etm.2020.8814
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