MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5

  • Authors:
    • Na Yue
    • Ming Ye
    • Ran Zhang
    • Miao Wang
  • View Affiliations

  • Published online on: May 28, 2020     https://doi.org/10.3892/etm.2020.8814
  • Pages: 1746-1751
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Abstract

The aim of the present study was to explore the roles of microRNA‑1307‑3p (miR‑1307‑3p) in colorectal cancer (CRC). Firstly, the expression level of miR‑1307‑3p in CRC cells was measured using reverse transcription‑quantitative PCR. Subsequently, Cell Counting Kit‑8 and Transwell invasion assays were performed to evaluate the effects of miR‑1307‑3p on CRC cell proliferation and invasion, respectively. Bioinformatics tools and dual luciferase reporter assays were used to validate the targets of miR‑1307‑3p. Rescue experiments were performed to confirm tumor suppressor candidate 5 (TUSC5) as a functional target of miR‑1307‑3p. miR‑1307‑3p levels were revealed to be upregulated in CRC cells when compared with the normal human epithelial cell line. Knockdown of miR‑1307‑3p inhibited CRC cell growth and invasiveness. Bioinformatics analysis and dual‑luciferase activity reporter assays demonstrated that miR‑1307‑3p binds the 3'‑untranslated region of TUSC5. Finally, rescue experiments validated that miR‑1307‑3p was able to regulate CRC cell behaviors via regulating TUSC5 expression. Together, the current results indicate that miR‑1307‑3p functions as an oncogenic miRNA via targeting TUSC5 in CRC.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Yue, N., Ye, M., Zhang, R., & Wang, M. (2020). MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5. Experimental and Therapeutic Medicine, 20, 1746-1751. https://doi.org/10.3892/etm.2020.8814
MLA
Yue, N., Ye, M., Zhang, R., Wang, M."MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5". Experimental and Therapeutic Medicine 20.2 (2020): 1746-1751.
Chicago
Yue, N., Ye, M., Zhang, R., Wang, M."MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1746-1751. https://doi.org/10.3892/etm.2020.8814