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Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells

  • Authors:
    • Zhi Hui Li
    • Zhanli Shi
    • Shengjie Tang
    • Hang Ping Yao
    • Xihua Lin
    • Fang Wu
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    Affiliations: Department of Intensive Care Unit, Hangzhou Red Cross Hospital/Hospital of Integrated Traditional Chinese and Western Medicine in Zhejiang Province, Hangzhou, Zhejiang 310003, P.R. China, Department of Endocrinology, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1621-1629
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    Published online on: June 3, 2020
       https://doi.org/10.3892/etm.2020.8827
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Abstract

The inflammatory response has been implicated in various cardiac and systemic diseases. Epigallocatechin‑3‑gallate (EGCG), the major polyphenol extracted from green tea, has various biological and pharmacological properties, such as anti‑inflammation, anti‑oxidative and anti‑tumorigenesis. To some extent, the mechanism of EGCG in the inflammatory response that characterizes myocardial dysfunction is not fully understood. The present study aimed to investigate the inhibiting effect of EGCG on lipopolysaccharide (LPS)‑induced inflammation in vitro. Treatment with LPS affected rat H9c2 cardiomyocytes and induced an inflammatory response. However, the LPS‑induced effects were attenuated after treatment with EGCG. The present results demonstrated that EGCG treatment repressed several inflammatory mediators, such as vascular endothelial growth factor, chemokine ligand 5, chemokine ligand 2, intercellular adhesion molecule‑1, matrix metalloproteinase‑2, tumor necrosis factor‑α and nitric oxide (induced by LPS), and the repressing effect of EGCG on inflammatory response was dose‑dependent in the range of 6.25‑100 µM. EGCG inhibited these marked inflammatory key signaling molecules by reducing the expression of phospho‑nuclear factor‑κB p65, ‑Akt, ‑ERK and ‑MAPK p38 while the total protein level of these signal proteins were not affected. In conclusion, the present findings suggested that EGCG possesses cardiomyocyte‑protective action in reducing the LPS‑induced inflammatory response due to the inhibition of the phosphorylation of Akt and ERK signaling molecules.
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Copy and paste a formatted citation
Spandidos Publications style
Li Z, Shi Z, Tang S, Yao H, Lin X and Wu F: Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells. Exp Ther Med 20: 1621-1629, 2020.
APA
Li, Z., Shi, Z., Tang, S., Yao, H., Lin, X., & Wu, F. (2020). Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells. Experimental and Therapeutic Medicine, 20, 1621-1629. https://doi.org/10.3892/etm.2020.8827
MLA
Li, Z., Shi, Z., Tang, S., Yao, H., Lin, X., Wu, F."Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells". Experimental and Therapeutic Medicine 20.2 (2020): 1621-1629.
Chicago
Li, Z., Shi, Z., Tang, S., Yao, H., Lin, X., Wu, F."Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1621-1629. https://doi.org/10.3892/etm.2020.8827
Copy and paste a formatted citation
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Spandidos Publications style
Li Z, Shi Z, Tang S, Yao H, Lin X and Wu F: Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells. Exp Ther Med 20: 1621-1629, 2020.
APA
Li, Z., Shi, Z., Tang, S., Yao, H., Lin, X., & Wu, F. (2020). Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells. Experimental and Therapeutic Medicine, 20, 1621-1629. https://doi.org/10.3892/etm.2020.8827
MLA
Li, Z., Shi, Z., Tang, S., Yao, H., Lin, X., Wu, F."Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells". Experimental and Therapeutic Medicine 20.2 (2020): 1621-1629.
Chicago
Li, Z., Shi, Z., Tang, S., Yao, H., Lin, X., Wu, F."Epigallocatechin‑3‑gallate ameliorates LPS‑induced inflammation by inhibiting the phosphorylation of Akt and ERK signaling molecules in rat H9c2 cells". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1621-1629. https://doi.org/10.3892/etm.2020.8827
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