MicroRNA‑27a inhibits trophoblast cell migration and invasion by targeting SMAD2: Potential role in preeclampsia

Zheng,Wenfei; Chen,Aihua; Yang,Huaijie; Hong,Li

doi:10.3892/etm.2020.8924

MicroRNA‑27a inhibits trophoblast cell migration and invasion by targeting SMAD2: Potential role in preeclampsia

Authors:
- Wenfei Zheng
- Aihua Chen
- Huaijie Yang
- Li Hong
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Affiliations: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Gynecology and Obstetrics, The People's Hospital of China Three Gorges University, Yichang, Hubei 443000, P.R. China
Published online on: June 24, 2020 https://doi.org/10.3892/etm.2020.8924
Pages: 2262-2269

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Abstract

Preeclampsia (PE) is a severe idiopathic obstetric complication that occurs worldwide. Insufficient trophoblast invasion is a characteristic of the pathogenesis of PE. MicroRNA‑27a (miR‑27a) has been reported to be highly expressed in PE placentas. The aim of the present study was to investigate the role and underlying mechanisms of miR‑27a in the pathogenesis of PE. The expression level of miR‑27a was evaluated in the placenta and serum from patients with PE and healthy pregnant women. Cell Counting Kit‑8 and flow cytometry assays were performed to detect human HTR‑8/SVneo trophoblast proliferation and apoptosis after miR‑27a overexpression or inhibition. In addition, Transwell assays were used to measure cell migration and invasion. A luciferase reporter assay was performed to determine the interaction between miR‑27a and SMAD2. The present results suggested that miR‑27a expression level was significantly increased in PE placentas and serum. In addition, miR‑27a overexpression suppressed cell migratory and invasive abilities, impaired proliferation and promoted apoptosis in human trophoblasts. It was demonstrated that miR‑27a may target SMAD and contribute to trophoblast invasion. Collectively, the results of the present study suggested that miR‑27a inhibited trophoblast cell migration and invasion by targeting SMAD2, thus presenting a promising therapeutic target for PE.

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