Open Access

cAMP regulates 11β‑hydroxysteroid dehydrogenase‑2 and Sp1 expression in MLO‑Y4/MC3T3‑E1 cells

  • Authors:
    • Di Liu
    • Yaoqing Wang
    • Zhenyu Pan
    • Zhen Huang
    • Fan Chen
  • View Affiliations

  • Published online on: June 25, 2020     https://doi.org/10.3892/etm.2020.8942
  • Pages: 2166-2172
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

11β-hydroxysteroid dehydrogenase-2 (11β-HSD2) is one of the key enzymes in glucocorticoid metabolism, which can inactivate local corticosterone and regulate the level of active glucocorticoid in tissues. The expression of 11β-HSD2 and its regulatory pathway serve an important role in the apoptosis of steroid induced osteonecrosis of the femoral head (SANFH). The present study aimed to identify the regulatory effects of cAMP on the expression of Sp1 transcription factor (Sp1) and 11β-HSD2 in osteocytes at the cellular level. Murine long bone osteocyte Y4 (MLO‑Y4) clone cells and mouse embryo osteoblast‑like (MC3T3‑E1) cells were cultured in vitro with adenylate cyclase activator or inhibitor (forskolin and SQ22536, respectively) to investigate the effects of alterations to intracellular cAMP levels. mRNA and protein expression levels of Sp1 and 11β-HSD2 were detected by reverse transcription‑quantitative PCR and western blotting, respectively. Compared with the negative control group, the mRNA and protein expression levels of Sp1 were significantly increased in the activation group, whereas Sp1 expression levels were significantly decreased in the inhibition group. Similarly, compared with the negative control group, the mRNA and protein expression levels of 11β-HSD2 were significantly increased in the activator group, but significantly decreased in the inhibitor group. The aforementioned results indicated that intracellular cAMP levels significantly regulated the expression of Sp1 and 11β-HSD2 in mouse osteocytes and osteoblasts. Therefore, the present study suggested a potential therapeutic strategy for the prevention of osteonecrosis of the femoral head.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu D, Wang Y, Pan Z, Huang Z and Chen F: cAMP regulates 11β‑hydroxysteroid dehydrogenase‑2 and Sp1 expression in MLO‑Y4/MC3T3‑E1 cells. Exp Ther Med 20: 2166-2172, 2020
APA
Liu, D., Wang, Y., Pan, Z., Huang, Z., & Chen, F. (2020). cAMP regulates 11β‑hydroxysteroid dehydrogenase‑2 and Sp1 expression in MLO‑Y4/MC3T3‑E1 cells. Experimental and Therapeutic Medicine, 20, 2166-2172. https://doi.org/10.3892/etm.2020.8942
MLA
Liu, D., Wang, Y., Pan, Z., Huang, Z., Chen, F."cAMP regulates 11β‑hydroxysteroid dehydrogenase‑2 and Sp1 expression in MLO‑Y4/MC3T3‑E1 cells". Experimental and Therapeutic Medicine 20.3 (2020): 2166-2172.
Chicago
Liu, D., Wang, Y., Pan, Z., Huang, Z., Chen, F."cAMP regulates 11β‑hydroxysteroid dehydrogenase‑2 and Sp1 expression in MLO‑Y4/MC3T3‑E1 cells". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2166-2172. https://doi.org/10.3892/etm.2020.8942