Open Access

Role of long non‑coding RNA MALAT1 in chronic obstructive pulmonary disease

  • Authors:
    • Tian‑Jun Hu
    • Hong‑Bo Huang
    • Hai‑Bo Shen
    • Wei Chen
    • Zhen‑Hua Yang
  • View Affiliations

  • Published online on: July 13, 2020     https://doi.org/10.3892/etm.2020.8996
  • Pages: 2691-2697
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic obstructive pulmonary disease (COPD) is a pathological inflammatory condition of the lungs that is associated with high rates of mortality. Although long non‑coding RNAs (lncRNAs) serve a role in lung diseases, their functions in COPD pathogenesis are relatively unknown. The present study aimed to assess the role of differentially expressed lncRNAs in COPD. Expression profile analysis of six lncRNAs in age‑matched COPD and non‑COPD tissues were conducted. Among the six tested lncRNAs, metastasis‑associated in lung adenocarcinoma transcript 1 (MALAT1) was the most consistently overexpressed in COPD lung tissue specimens. To model COPD in vitro, human lung fibroblasts were treated with transforming growth factor‑β (TGF‑β) and MALAT1 was knocked down by small interfering RNA. This promoted cell viability and concurrently inhibited the expression of mesenchymal proteins, fibronectin and α‑smooth muscle actin. In COPD, cell senescence is linked to the activation of mammalian target of rapamycin complex 1 (mTORC1). Upon gene silencing of MALAT1 in non‑TGF‑β‑treated cells, cells demonstrated constitutive activation of mTORC1, which was assessed by the protein expression levels of mTORC1 substrate S6 kinase (S6K1). By contrast, upon MALAT1 silencing in the TGF‑β‑treated cells, mTORC1 activation was not suppressed, despite the mesenchymal cell markers protein expression levels being downregulated. Thus, lncRNA MALAT1 may represent a potent biomarker in COPD patients and may act as a target for both diagnostic and therapeutic purposes.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hu TJ, Huang HB, Shen HB, Chen W and Yang ZH: Role of long non‑coding RNA MALAT1 in chronic obstructive pulmonary disease. Exp Ther Med 20: 2691-2697, 2020
APA
Hu, T., Huang, H., Shen, H., Chen, W., & Yang, Z. (2020). Role of long non‑coding RNA MALAT1 in chronic obstructive pulmonary disease. Experimental and Therapeutic Medicine, 20, 2691-2697. https://doi.org/10.3892/etm.2020.8996
MLA
Hu, T., Huang, H., Shen, H., Chen, W., Yang, Z."Role of long non‑coding RNA MALAT1 in chronic obstructive pulmonary disease". Experimental and Therapeutic Medicine 20.3 (2020): 2691-2697.
Chicago
Hu, T., Huang, H., Shen, H., Chen, W., Yang, Z."Role of long non‑coding RNA MALAT1 in chronic obstructive pulmonary disease". Experimental and Therapeutic Medicine 20, no. 3 (2020): 2691-2697. https://doi.org/10.3892/etm.2020.8996