EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway

Mou,Qiuju; Jiang,Yan; Zhu,Lili; Zhu,Zixin; Ren,Tingting

doi:10.3892/etm.2020.9047

EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway

Authors:
- Qiuju Mou
- Yan Jiang
- Lili Zhu
- Zixin Zhu
- Tingting Ren
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Affiliations: Department of Blood Transfusion, The Affiliated Baiyun Hospital of Guizhou Medical University, Guiyang, Guizhou 550014, P.R. China, Department of Microbiology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Blood Transfusion, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, School of Basic Medicine Science, Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China, Department of Physiology Chemistry, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
Published online on: July 24, 2020 https://doi.org/10.3892/etm.2020.9047
Pages: 3017-3024
Copyright: © Mou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epigallocatechin gallate (EGCG) is the main component of green tea, which has been proven to inhibit a variety of viruses, including influenza A virus. However, the mechanism of EGCG against influenza virus remains to be further explored. The mechanism of EGCG against influenza virus was studied. The results showed that EGCG significantly increased the levels of HBD3 mRNA and protein, while the levels of phosphorylation of (p)-p38 MAPK, ERK and JNK after EGCG treatment were significantly up-regulated. p38 MAPK, ERK and JNK inhibitors significantly inhibited the expression of HBD3 induced by EGCG. On the other hand, EGCG significantly inhibited the expression of HA and NP proteins in influenza A virus H1N1, but attenuated the anti‑influenza A virus effect of EGCG after silencing HBD3. Thus, the anti-influenza virus effect of EGCG is related to the induction of HBD3 expression. In addition, the expression of EGCG-induced HBD3 is related to the p38 MAPK, ERK and JNK signaling pathways. The research data show that EGCG can induce HBD3 expression through p38 MAPK, ERK and JNK signaling pathway to inhibit the replication of influenza A virus H1N1, providing a new and effective candidate drug for influenza virus.

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