Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2020 Volume 20 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2020 Volume 20 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway

  • Authors:
    • Qiuju Mou
    • Yan Jiang
    • Lili Zhu
    • Zixin Zhu
    • Tingting Ren
  • View Affiliations / Copyright

    Affiliations: Department of Blood Transfusion, The Affiliated Baiyun Hospital of Guizhou Medical University, Guiyang, Guizhou 550014, P.R. China, Department of Microbiology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, Department of Blood Transfusion, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China, School of Basic Medicine Science, Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China, Department of Physiology Chemistry, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Mou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3017-3024
    |
    Published online on: July 24, 2020
       https://doi.org/10.3892/etm.2020.9047
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Epigallocatechin gallate (EGCG) is the main component of green tea, which has been proven to inhibit a variety of viruses, including influenza A virus. However, the mechanism of EGCG against influenza virus remains to be further explored. The mechanism of EGCG against influenza virus was studied. The results showed that EGCG significantly increased the levels of HBD3 mRNA and protein, while the levels of phosphorylation of (p)-p38 MAPK, ERK and JNK after EGCG treatment were significantly up-regulated. p38 MAPK, ERK and JNK inhibitors significantly inhibited the expression of HBD3 induced by EGCG. On the other hand, EGCG significantly inhibited the expression of HA and NP proteins in influenza A virus H1N1, but attenuated the anti‑influenza A virus effect of EGCG after silencing HBD3. Thus, the anti-influenza virus effect of EGCG is related to the induction of HBD3 expression. In addition, the expression of EGCG-induced HBD3 is related to the p38 MAPK, ERK and JNK signaling pathways. The research data show that EGCG can induce HBD3 expression through p38 MAPK, ERK and JNK signaling pathway to inhibit the replication of influenza A virus H1N1, providing a new and effective candidate drug for influenza virus.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Horimoto T and Kawaoka Y: Influenza: Lessons from past pandemics, warnings from current incidents. Nat Rev Microbiol. 3:591–600. 2005.PubMed/NCBI View Article : Google Scholar

2 

Hause BM, Collin EA, Liu R, Huang B, Sheng Z, Lu W, Wang D, Nelson EA and Li F: Characterization of a novel influenza virus in cattle and Swine: Proposal for a new genus in the Orthomyxoviridae family. MBio. 5:e00031–e14. 2014.PubMed/NCBI View Article : Google Scholar

3 

Lee VJ, Ho ZJM, Goh EH, Campbell H, Cohen C, Cozza V, Fitzner J, Jara J, Krishnan A and Bresee J: WHO Working Group on Influenza Burden of Disease. Advances in measuring influenza burden of disease. Influenza Other Respir Viruses. 12:3–9. 2018.PubMed/NCBI View Article : Google Scholar

4 

Mungall BA, Xu X and Klimov A: Surveillance of influenza isolates for susceptibility to neuraminidase inhibitors during the 2000-2002 influenza seasons. Virus Res. 103:195–197. 2004.PubMed/NCBI View Article : Google Scholar

5 

Nguyen JT, Smee DF, Barnard DL, Julander JG, Gross M, de Jong MD and Went GT: Efficacy of combined therapy with amantadine, oseltamivir, and ribavirin in vivo against susceptible and amantadine-resistant influenza A viruses. PLoS One. 7(e31006)2012.PubMed/NCBI View Article : Google Scholar

6 

Pazgier M, Li X, Lu W and Lubkowski J: Human defensins: Synthe- sis and structural properties. Curr Pharm Des. 13:3096–3118. 2007.PubMed/NCBI View Article : Google Scholar

7 

Shah R and Chang TL: Defensins in viral infection. In: Small Wonders: Peptides for Disease Control. Rajasekaran K, Cary JW, Jaynes JM, Montesinos E (eds). Vol 1095. ACS Symposium Series, pp137-171, 2012. https://doi.org/10.1021/bk-2012-1095.ch007.

8 

Jiang Y, Yang D, Li W, Wang B, Jiang Z and Li M: Antiviral activity of recombinant mouse β-defensin 3 against influenza A virus in vitro and in vivo. Antivir Chem Chemother. 22:255–262. 2012.PubMed/NCBI View Article : Google Scholar

9 

Jiang Y, Wang Y, Kuang Y, Wang B, Li W, Gong T, Jiang Z, Yang D and Li M: Expression of mouse β-defensin-3 in MDCK cells and its anti-influenza-virus activity. Arch Virol. 154:639–647. 2009.PubMed/NCBI View Article : Google Scholar

10 

Yang SH, Sharrocks AD and Whitmarsh AJ: MAP kinase signalling cascades and transcriptional regulation. Gene. 513:1–13. 2013.PubMed/NCBI View Article : Google Scholar

11 

Haarmann H, Steiner T, Schreiber F, Heinrich A, Zweigner J, N’Guessan PD and Slevogt H: The role and regulation of Moraxella catarrhalis-induced human beta-defensin 3 expression in human pulmonary epithelial cells. Biochem Biophys Res Commun. 467:46–52. 2015.PubMed/NCBI View Article : Google Scholar

12 

Chacko SM, Thambi PT, Kuttan R and Nishigaki I: Beneficial effects of green tea: A literature review. Chin Med. 5(13)2010.PubMed/NCBI View Article : Google Scholar

13 

Kim M, Kim SY, Lee HW, Shin JS, Kim P, Jung YS, Jeong HS, Hyun JK and Lee CK: Inhibition of influenza virus internalization by (-)-epigallocatechin-3-gallate. Antiviral Res. 100:460–472. 2013.PubMed/NCBI View Article : Google Scholar

14 

Gan Y, Cui X, Ma T, Liu Y, Li A and Huang M: Paeoniflorin upregulates β-defensin-2 expression in human bronchial epithelial cell through the p38 MAPK, ERK, and NF-κB signaling pathways. Inflammation. 37:1468–1475. 2014.PubMed/NCBI View Article : Google Scholar

15 

Fusco A, Savio V, Cammarota M, Alfano A, Schiraldi C and Donnarumma G: Schiraldi C and Donnarumma G: Beta- defensin-2 and beta-defensin-3 reduce intestinal damage caused by Salmonella typhimurium modulating the expression of cytokines and enhancing the probiotic activity of Enterococcus faecium. J Immunol Res. 2017(6976935)2017.PubMed/NCBI View Article : Google Scholar

16 

Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB Jr and Ganz T: Human beta-defensin-1: An antimicrobial peptide of urogenital tissues. J Clin Invest. 101:1633–1642. 1998.PubMed/NCBI View Article : Google Scholar

17 

Reichel M, Heisig A, Heisig P and Kampf G: Skin bacteria after chlorhexidine exposure-is there a difference in response to human beta-Defensin-3? Eur J Clin Microbiol Infect Dis. 29:623–632. 2010.PubMed/NCBI View Article : Google Scholar

18 

Bezniakow N, Gos M and Obersztyn E: The RASopathies as an example of RAS/MAPK pathway disturbances - clinical presentation and molecular pathogenesis of selected syndromes. Dev Period Med. 18:285–296. 2014.PubMed/NCBI

19 

Kono M, Tatsumi K, Imai AM, Saito K, Kuriyama T and Shirasawa H: Kuriyama T and Shirasawa H: Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: Involvement of p38 MAPK and ERK. Antiviral Res. 77:150–152. 2008.PubMed/NCBI View Article : Google Scholar

20 

Sui X, Kong N, Ye L, Han W, Zhou J, Zhang Q, He C and Pan H: p38 and JNK MAPK pathways control the balance of apoptosis and autophagy in response to chemotherapeutic agents. Cancer Lett. 344:174–179. 2014.PubMed/NCBI View Article : Google Scholar

21 

Cerezo-Guisado MI, Zur R, Lorenzo MJ, Risco A, Martín-Serrano MA, Alvarez-Barrientos A, Cuenda A and Centeno F: Implication of Akt, ERK1/2 and alternative p38MAPK signalling pathways in human colon cancer cell apoptosis induced by green tea EGCG. Food Chem Toxicol. 84:125–132. 2015.PubMed/NCBI View Article : Google Scholar

22 

Boughan PK, Argent RH, Body-Malapel M, Park JH, Ewings KE, Bowie AG, Ong SJ, Cook SJ, Sorensen OE, Manzo BA, et al: Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: Critical regulators of beta-defensins during Helicobacter pylori infection. J Biol Chem. 281:11637–11648. 2006.PubMed/NCBI View Article : Google Scholar

23 

Liu J, Du X, Chen J, Hu L and Chen L: The induction expression of human β-defensins in gingival epithelial cells and fibroblasts. Arch Oral Biol. 58:1415–1421. 2013.PubMed/NCBI View Article : Google Scholar

24 

Paoletti I, Buommino E, Fusco A, Baudouin C, Msika P, Tufano MA, Baroni A and Donnarumma G: Patented natural avocado sugar modulates the HBD-2 and HBD-3 expression in human keratinocytes through toll-like receptor-2 and ERK/MAPK activation. Arch Dermatol Res. 304:619–625. 2012.PubMed/NCBI View Article : Google Scholar

25 

Colpitts CC and Schang LM: A small molecule inhibits virion attachment to heparan sulfate- or sialic acid-containing glycans. J Virol. 88:7806–7817. 2014.PubMed/NCBI View Article : Google Scholar

26 

Kesic MJ, Simmons SO, Bauer R and Jaspers I: Nrf2 expression modifies influenza A entry and replication in nasal epithelial cells. Free Radic Biol Med. 51:444–453. 2011.PubMed/NCBI View Article : Google Scholar

27 

Xu MJ, Liu BJ, Wang CL, Wang GH, Tian Y, Wang SH, Li J, Li PY, Zhang RH, Wei D, et al: Epigallocatechin-3-gallate inhibits TLR4 signaling through the 67-kDa laminin receptor and effectively alleviates acute lung injury induced by H9N2 swine influenza virus. Int Immunopharmacol. 52:24–33. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Mou Q, Jiang Y, Zhu L, Zhu Z and Ren T: EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway. Exp Ther Med 20: 3017-3024, 2020.
APA
Mou, Q., Jiang, Y., Zhu, L., Zhu, Z., & Ren, T. (2020). EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway. Experimental and Therapeutic Medicine, 20, 3017-3024. https://doi.org/10.3892/etm.2020.9047
MLA
Mou, Q., Jiang, Y., Zhu, L., Zhu, Z., Ren, T."EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway". Experimental and Therapeutic Medicine 20.4 (2020): 3017-3024.
Chicago
Mou, Q., Jiang, Y., Zhu, L., Zhu, Z., Ren, T."EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3017-3024. https://doi.org/10.3892/etm.2020.9047
Copy and paste a formatted citation
x
Spandidos Publications style
Mou Q, Jiang Y, Zhu L, Zhu Z and Ren T: EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway. Exp Ther Med 20: 3017-3024, 2020.
APA
Mou, Q., Jiang, Y., Zhu, L., Zhu, Z., & Ren, T. (2020). EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway. Experimental and Therapeutic Medicine, 20, 3017-3024. https://doi.org/10.3892/etm.2020.9047
MLA
Mou, Q., Jiang, Y., Zhu, L., Zhu, Z., Ren, T."EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway". Experimental and Therapeutic Medicine 20.4 (2020): 3017-3024.
Chicago
Mou, Q., Jiang, Y., Zhu, L., Zhu, Z., Ren, T."EGCG induces β-defensin 3 against influenza A virus H1N1 by the MAPK signaling pathway". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3017-3024. https://doi.org/10.3892/etm.2020.9047
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team