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Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation

  • Authors:
    • Bei Yan
    • Xinzong Zhang
    • Juan Wang
    • Shaotong Jia
    • Yue Zhou
    • Jia Tian
    • Hongyan Wang
    • Yunge Tang
  • View Affiliations / Copyright

    Affiliations: Ningxia Human Sperm Bank, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750001, P.R. China, National Health Committee Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong Province, Guangzhou, Guangdong 510600, P.R. China, Department of Pathology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Reproductive Center, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750001, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3051-3063
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    Published online on: July 28, 2020
       https://doi.org/10.3892/etm.2020.9060
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Abstract

With the development of cryopreservation technology, marked progress has been made regarding sperm cryopreservation. However, as conventional cryopreservation agents are not effective at freezing weak sperm, improved cryopreservation agents are in demand. In the present study, the addition of Lycium barbarum polysaccharides to glycerol‑egg‑yolk‑citrate sperm cryopreservation agent was determined to improve sperm forward speed, reduce the DNA fragmentation index and improve the mitochondrial membrane potential. Furthermore, during the freezing and thawing of sperm, the improved cryopreservative increased the content of Bcl‑2 while reducing the content of Bax, cytochrome C and caspase‑3. These results indicated that polysaccharides added as a protective agent preserved the normal function of sperm mitochondria. Transmission electron microscopy also confirmed the protective effect of the polysaccharides on the structure of mitochondria. It was also indicated that improved cryopreservative lowered the levels of reactive oxygen species (ROS) during the freeze‑thawing process. Therefore, it is hypothesized that improved cryopreservative agents may be beneficial for maintaining the structure and function of the mitochondria of weak sperm when cryopreserved, which may be facilitated via reducing the production of ROS in the freezing‑thawing process, thus avoiding activation of the apoptotic pathway in sperm mitochondria and protecting mitochondrial structure and sperm function.
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Copy and paste a formatted citation
Spandidos Publications style
Yan B, Zhang X, Wang J, Jia S, Zhou Y, Tian J, Wang H and Tang Y: Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation. Exp Ther Med 20: 3051-3063, 2020.
APA
Yan, B., Zhang, X., Wang, J., Jia, S., Zhou, Y., Tian, J. ... Tang, Y. (2020). Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation. Experimental and Therapeutic Medicine, 20, 3051-3063. https://doi.org/10.3892/etm.2020.9060
MLA
Yan, B., Zhang, X., Wang, J., Jia, S., Zhou, Y., Tian, J., Wang, H., Tang, Y."Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation". Experimental and Therapeutic Medicine 20.4 (2020): 3051-3063.
Chicago
Yan, B., Zhang, X., Wang, J., Jia, S., Zhou, Y., Tian, J., Wang, H., Tang, Y."Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3051-3063. https://doi.org/10.3892/etm.2020.9060
Copy and paste a formatted citation
x
Spandidos Publications style
Yan B, Zhang X, Wang J, Jia S, Zhou Y, Tian J, Wang H and Tang Y: Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation. Exp Ther Med 20: 3051-3063, 2020.
APA
Yan, B., Zhang, X., Wang, J., Jia, S., Zhou, Y., Tian, J. ... Tang, Y. (2020). Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation. Experimental and Therapeutic Medicine, 20, 3051-3063. https://doi.org/10.3892/etm.2020.9060
MLA
Yan, B., Zhang, X., Wang, J., Jia, S., Zhou, Y., Tian, J., Wang, H., Tang, Y."Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation". Experimental and Therapeutic Medicine 20.4 (2020): 3051-3063.
Chicago
Yan, B., Zhang, X., Wang, J., Jia, S., Zhou, Y., Tian, J., Wang, H., Tang, Y."Inhibitory effect of Lycium barbarum polysaccharide on sperm damage during cryopreservation". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3051-3063. https://doi.org/10.3892/etm.2020.9060
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