Open Access

Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine

  • Authors:
    • Xiao‑Juan Ren
    • Qing‑Quan Wang
    • Xing‑Ping Zhang
    • Guan‑Ying Wang
    • Tao Liu
    • Ning Deng
    • De‑Qi Yan
  • View Affiliations

  • Published online on: July 29, 2020     https://doi.org/10.3892/etm.2020.9080
  • Pages: 3228-3236
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The current study aimed to establish a rat model of ageing insomnia induced by D‑galactose and/or para‑chlorophenylalanine. Following establishment of the model, body weights were measured, and Morris water maze and pentobarbital‑induced sleep tests were performed. The serum levels of inflammatory mediators and the neural levels of neurotransmitters were detected. The results demonstrated that the body weights of PCPA+D‑gal‑induced ageing insomnia rats decreased significantly. Ageing insomnia rats exhibited longer latencies to the platform in the Morris water maze tests and fewer target crossings. The sleep latency of the model rats was longer and sleep time was shorter by contrast. The relative expression of hippocampal IL‑6, TNF‑α, NF‑κB and mGluR2 mRNA of the PCPA+D‑gal‑induced ageing insomnia group was higher, while the relative expression of 5‑HT1AR and GABAARa1 mRNA were lower. The serum levels of IL‑1β, IL‑6, TNF‑α and brain level of glutamate increased in the PCPA+D‑gal‑induced ageing insomnia group, while the levels of 5‑HT and GABA decreased. In conclusion, memory function, sleep time, expression of inflammatory factors and neurotransmitters are altered in ageing insomnia rats induced by D‑galactose and para‑chlorophenylalanine, indicating the successful establishment of a murine model of ageing insomnia.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ren XJ, Wang QQ, Zhang XP, Wang GY, Liu T, Deng N and Yan DQ: Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine. Exp Ther Med 20: 3228-3236, 2020
APA
Ren, X., Wang, Q., Zhang, X., Wang, G., Liu, T., Deng, N., & Yan, D. (2020). Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine. Experimental and Therapeutic Medicine, 20, 3228-3236. https://doi.org/10.3892/etm.2020.9080
MLA
Ren, X., Wang, Q., Zhang, X., Wang, G., Liu, T., Deng, N., Yan, D."Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine". Experimental and Therapeutic Medicine 20.4 (2020): 3228-3236.
Chicago
Ren, X., Wang, Q., Zhang, X., Wang, G., Liu, T., Deng, N., Yan, D."Establishment of a rat model with ageing insomnia induced by D‑galactosef and para‑chlorophenylalanine". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3228-3236. https://doi.org/10.3892/etm.2020.9080